Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,764,190	A→G	F322L (TTC→CTC)	yfjL ←	CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,764,190	0	A	G	100.0%	93.0 / NA	25	F322L (TTC→CTC)	yfjL	CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/13);  total (12/13)

CTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGC  >  NC_000913/2764043‑2764333                                                                                                                                                    |                                                                                                                                                cTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAgtgt                                                                                                                                              >  1:223842/1‑151 (MQ=255)  tttttCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTc                                                                                                                                             >  2:632644/1‑151 (MQ=255)       ccACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGttt                                                                                                                                        <  1:128068/151‑1 (MQ=255)       ccACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGttt                                                                                                                                        <  2:400393/151‑1 (MQ=255)       ccACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGttt                                                                                                                                        <  1:343947/151‑1 (MQ=255)         aCGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTAt                                                                                                                                      <  2:76933/151‑1 (MQ=255)           gTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGa                                                                                                                                    <  1:235691/151‑1 (MQ=255)                    gCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGa                                                                                                                            <  1:667514/150‑1 (MQ=255)                                  ggctTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCg                                                                                                             >  1:8478/1‑151 (MQ=255)                                     tttCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCgg                                                                                                            >  2:201073/1‑149 (MQ=255)                                      ttCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTTTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCgta                                                                                                           <  1:235367/149‑3 (MQ=255)                                      ttCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGgag                                                                                                          <  1:225263/150‑1 (MQ=255)                                          tggtgCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGaaaa                                                                                                      >  2:193944/1‑150 (MQ=255)                                             tgctgcCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTATCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGaaaa                                                                                                      >  1:516830/1‑147 (MQ=255)                                                                 cAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACg                                                                              >  1:297690/1‑151 (MQ=255)                                                                     aGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTCTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTAt                                                                          <  2:394761/151‑1 (MQ=255)                                                                       cACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATcc                                                                        <  2:239129/151‑1 (MQ=255)                                                                                   aaaTTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTg                                                            >  1:593229/1‑151 (MQ=255)                                                                                         gCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCgg                                                      >  2:149093/1‑151 (MQ=255)                                                                                                        cGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAg                                       <  2:73611/151‑1 (MQ=255)                                                                                                               aaGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTActgct                                <  2:589677/151‑1 (MQ=255)                                                                                                                aGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTActgctg                               >  2:311853/1‑151 (MQ=255)                                                                                                                                 cGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCt               <  1:670716/150‑1 (MQ=255)                                                                                                                                 cGTTCTCGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCt               >  2:670727/1‑150 (MQ=255)                                                                                                                                    tctcGCTTTTCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTAc           >  1:285986/1‑151 (MQ=255)                                                                                                                                             tCAATGAGTGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAgcgc  >  2:636589/1‑151 (MQ=255)                                                                                                                                               aatgagtgTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAgcgc  >  2:312440/1‑149 (MQ=255)                                                                                                                                                    |                                                                                                                                                CTTTTTCCACGTATAGCATGCAAGGAATAGGCTGGCTTTCGTGGTGCTGCCGGATCGTTGCGTGCAACAGCACTCGACCCAAAAATTCGCTGACGTCATGGAGCGATTTGAAGACGGAATTCTTTAGTCGTTCTCGCTTTTCAATGAATGTCAGTTTATGAATATCAGACATTTCTTTTGGTCGGAGAAAAACCTCATCCAACCATTGACAGACGTTATCCGCGATGTGCTTGTAGCGGTATTTCTTAAGTGAGTACTGCTGATGTAGAACCTGGCCTTTACGCTTAGCGC  >  NC_000913/2764043‑2764333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑