Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,767,140	T→C	L53L (TTG→CTG)	rnlB →	CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,767,140	0	T	C	100.0%	131.5 / NA	35	L53L (TTG→CTG)	rnlB	CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/15);  total (20/15)

CCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCAT  >  NC_000913/2766997‑2767286                                                                                                                                                |                                                                                                                                                   ccGGAATTAACGTCTCAGGGGCTCTAAAAGCTGGCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTgcgc                                                                                                                                             >  1:484940/1‑151 (MQ=255)  cGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCt                                                                                                                                            <  2:570671/151‑1 (MQ=255)         aaCGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTc                                                                                                                                     <  1:551191/151‑1 (MQ=255)           cGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCa                                                                                                                                   <  1:321325/151‑1 (MQ=255)              ctcAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCag                                                                                                                                  >  1:11354/1‑149 (MQ=255)                cAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGt                                                                                                                               <  1:294795/150‑1 (MQ=255)                   gggCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGa                                                                                                                            >  1:625230/1‑150 (MQ=255)                   gggCGCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGCACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGa                                                                                                                            >  1:34498/1‑150 (MQ=255)                     gCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAAcc                                                                                                                         <  2:624036/151‑1 (MQ=255)                        ctAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTTATACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGCCCTCCTGTGCGCTAACGGTCCAGAGTGGAACCg                                                                                                                        <  1:463976/149‑1 (MQ=255)                                   cGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTg                                                                                                           <  2:506825/151‑1 (MQ=255)                                    gTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTgg                                                                                                          <  2:11353/151‑1 (MQ=255)                                      tATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGga                                                                                                         >  2:118417/1‑150 (MQ=255)                                      tATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGga                                                                                                         <  1:654732/150‑1 (MQ=255)                                                   ttCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGcc                                                                                           >  1:335621/1‑151 (MQ=255)                                                        gaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATc                                                                                      >  2:72930/1‑151 (MQ=255)                                                           cccTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATg                                                                                   >  2:561345/1‑151 (MQ=255)                                                            ccTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGc                                                                                  <  2:496488/151‑1 (MQ=255)                                                              tttGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTg                                                                                >  1:391277/1‑151 (MQ=255)                                                                   ccTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATAc                                                                           >  2:494850/1‑151 (MQ=255)                                                                       aGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGcc                                                                       >  2:344629/1‑151 (MQ=255)                                                                               aaaTTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAattat                                                               >  1:191193/1‑151 (MQ=255)                                                                                      gaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatg                                                          >  2:509594/1‑149 (MQ=255)                                                                                       aCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGc                                                       <  2:420333/151‑1 (MQ=255)                                                                                         gAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGc                                                       >  2:409980/1‑149 (MQ=255)                                                                                                tCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGtt                                               <  1:579170/150‑1 (MQ=255)                                                                                                tCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGtt                                               <  2:408898/150‑1 (MQ=255)                                                                                                   gCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTc                                              <  1:408934/148‑1 (MQ=255)                                                                                                       tACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGacac                                        >  2:487707/1‑150 (MQ=255)                                                                                                       tACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGacac                                        >  2:278932/1‑150 (MQ=255)                                                                                                         cTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACat                                      >  1:336265/1‑150 (MQ=255)                                                                                                                       cACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTg                       <  2:649946/151‑1 (MQ=255)                                                                                                                              gAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACtttt                 >  2:137234/1‑150 (MQ=255)                                                                                                                                 aTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttactt             >  1:250122/1‑151 (MQ=255)                                                                                                                                         tGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAat     >  2:443164/1‑151 (MQ=255)                                                                                                                                            cctcctGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAatcat  <  1:29603/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCAT  >  NC_000913/2766997‑2767286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑