Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,780,330	G→A	G799G (GGC→GGT)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,780,330	0	G	A	100.0%	143.0 / NA	37	G799G (GGC→GGT)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (24/13);  total (24/13)

TATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGC  >  NC_000913/2780189‑2780473                                                                                                                                              |                                                                                                                                                tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATAtt                                                                                                                                         >  1:607053/1‑150 (MQ=255) tATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTg                                                                                                                                        >  1:421864/1‑151 (MQ=255)      ccTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCtg                                                                                                                                   <  2:108597/151‑1 (MQ=255)             gTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGtttt                                                                                                                             <  2:206011/150‑1 (MQ=255)                   tGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCaga                                                                                                                       >  2:239366/1‑150 (MQ=255)                   tGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCaga                                                                                                                       >  1:339292/1‑150 (MQ=255)                          gTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGgtgt                                                                                                               >  1:282234/1‑151 (MQ=255)                          gTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGgtgt                                                                                                               >  1:343089/1‑151 (MQ=255)                            ttGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCt                                                                                                             >  1:138857/1‑151 (MQ=255)                             tGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCt                                                                                                             <  2:365043/150‑1 (MQ=255)                               tATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGa                                                                                                           <  1:429221/150‑1 (MQ=255)                                aTTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACt                                                                                                         >  2:277609/1‑151 (MQ=255)                                 ttCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACt                                                                                                         <  2:172022/150‑1 (MQ=255)                                          cgtTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTa                                                                                                >  2:164202/1‑150 (MQ=255)                                              aaTTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcg                                                                                           <  2:490675/151‑1 (MQ=255)                                                 tgttgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACAcgccg                                                                                        >  2:628844/1‑151 (MQ=255)                                                     gtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCa                                                                                     <  2:59670/150‑1 (MQ=255)                                                             cGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcag                                                                             <  2:666794/150‑1 (MQ=255)                                                             cGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCcagc                                                                            >  2:219240/1‑151 (MQ=255)                                                                   acCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGat                                                                       >  1:26768/1‑150 (MQ=255)                                                                     ccTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATAtt                                                                    >  1:428240/1‑151 (MQ=255)                                                                        cGACAATTTTTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGtt                                                                 <  1:249566/151‑1 (MQ=255)                                                                        cGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGtt                                                                 >  2:297791/1‑151 (MQ=255)                                                                           cAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgc                                                              >  2:570493/1‑151 (MQ=255)                                                                            aaTTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgcc                                                             >  1:215972/1‑151 (MQ=255)                                                                              tttGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgccg                                                            <  2:299541/150‑1 (MQ=255)                                                                               ttGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTcgccgc                                                           >  2:71684/1‑150 (MQ=255)                                                                                tGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAc                                                         >  1:551941/1‑151 (MQ=255)                                                                                tGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCAc                                                         >  1:137918/1‑151 (MQ=255)                                                                                    cACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTg                                                     <  2:350836/151‑1 (MQ=255)                                                                                                        ccTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGc                                 >  1:442064/1‑151 (MQ=255)                                                                                                           gTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGcc                                <  2:282233/149‑1 (MQ=255)                                                                                                           gTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCa                               <  2:83108/150‑1 (MQ=255)                                                                                                                  tGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCGGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcgcgcg                       >  2:383998/1‑151 (MQ=255)                                                                                                                    gCTTTTGTTTTGACGATCTCCCGTCCACCCGTATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCgt                      >  2:181147/1‑150 (MQ=255)                                                                                                                                aCGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGAccc         >  1:32762/1‑151 (MQ=255)                                                                                                                                       cccGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGc  >  1:16730/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                TATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCGTCGC  >  NC_000913/2780189‑2780473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑