Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	287,616	G→A	L276L (CTG→CTA)	yagH →	CP4‑6 prophage; putative xylosidase/arabinosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	287,616	0	G	A	100.0%	146.7 / NA	39	L276L (CTG→CTA)	yagH	CP4‑6 prophage; putative xylosidase/arabinosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/21);  total (18/21)

ACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGC  >  NC_000913/287474‑287758                                                                                                                                               |                                                                                                                                               aCGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTc                                                                                                                                        >  1:533341/1‑151 (MQ=255)      aCGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCcggcg                                                                                                                                   >  2:177610/1‑151 (MQ=255)       cGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCcggcgg                                                                                                                                  <  1:270507/151‑1 (MQ=255)              gaCCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGc                                                                                                                           >  2:639354/1‑151 (MQ=255)                 cAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTAc                                                                                                                        >  1:471300/1‑151 (MQ=255)                        cACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGc                                                                                                                 <  1:276398/151‑1 (MQ=255)                                    aaCCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCgaga                                                                                                     <  2:281501/151‑1 (MQ=255)                                     aCCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGAc                                                                                                    >  2:53526/1‑151 (MQ=255)                                     aCCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGAc                                                                                                    <  2:193133/151‑1 (MQ=255)                                      cccGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGAcc                                                                                                   <  1:221752/151‑1 (MQ=255)                                       ccGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCg                                                                                                  >  2:84972/1‑151 (MQ=255)                                         gCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGc                                                                                                <  2:104822/151‑1 (MQ=255)                                           tGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCAt                                                                                              >  2:476216/1‑151 (MQ=255)                                             cAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCAt                                                                                              <  1:258127/149‑1 (MQ=255)                                             cAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCg                                                                                            <  1:626208/151‑1 (MQ=255)                                               gaagaGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGc                                                                                           <  1:422611/150‑1 (MQ=255)                                                        ccACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGa                                                                                  >  2:10003/1‑150 (MQ=255)                                                                  ctgctgCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGAc                                                                        >  1:376632/1‑150 (MQ=255)                                                                   tgctgcAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACgg                                                                      >  1:318397/1‑151 (MQ=255)                                                                       gcAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTg                                                                   <  2:527973/150‑1 (MQ=255)                                                                         aGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGcc                                                                <  2:34957/151‑1 (MQ=255)                                                                          gACGCATACGGGTGAATGGTACATGGCCTACCTCACCACCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCg                                                               <  1:444713/151‑1 (MQ=255)                                                                            cGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGt                                                              >  2:522636/1‑150 (MQ=255)                                                                              cATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACg                                                           >  1:199980/1‑151 (MQ=255)                                                                              cATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACg                                                           <  1:36176/151‑1 (MQ=255)                                                                                   gggTGAATGGTACATGGCCTACCTCCCCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTTCGTGGaa                                                      <  1:197731/151‑1 (MQ=255)                                                                                   gggTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGa                                                       >  1:602970/1‑150 (MQ=255)                                                                                       gAACGGTACATGTCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAgg                                                    <  1:35388/149‑1 (MQ=255)                                                                                         aTGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAggcggc                                                <  2:471304/151‑1 (MQ=255)                                                                                           ggTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCa                                               >  1:236371/1‑150 (MQ=255)                                                                                                aaggCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCAcg                                         >  1:64367/3‑151 (MQ=255)                                                                                                       aCCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGc                                   <  2:297759/150‑1 (MQ=255)                                                                                                        ccTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTg                                 <  2:504176/151‑1 (MQ=255)                                                                                                               aGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTg                           >  2:592861/1‑150 (MQ=255)                                                                                                               aGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGa                          <  1:326268/151‑1 (MQ=255)                                                                                                                                 cccGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCg        <  1:361904/151‑1 (MQ=255)                                                                                                                                   cGGCGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCg        >  2:277090/1‑149 (MQ=255)                                                                                                                                      cGTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGagcag   <  1:359963/151‑1 (MQ=255)                                                                                                                                       gTGCCGCTACTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGagcagc  >  2:558946/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                               ACGTAACGATGATGACCAGCTGGCACCTGCCGGAGAACCCGCTGCAGAAGAGCGGCCACGGCTCGCTGCTGCAGACGCATACGGGTGAATGGTACATGGCCTACCTCACCAGCCGCCCGCTGCGCCTGCCCGGCGTGCCGCTGCTGGCCTCCGGCGGACGCGGCTACTGCCCGCTGGGGCGCGAGACCGGCATCGCCCGCATTGAATGGCGCGACGGCTGGCCGTACGTGGAAGGCGGCAAGCACGCGCAGCTGACCGTGAAAGGCCCGCAAGTAGCCGAGCAGC  >  NC_000913/287474‑287758

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑