Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,898,881	C→T	A180T (GCA→ACA)	ygcU ←	putative FAD‑linked oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,898,881	0	C	T	100.0%	128.8 / NA	34	A180T (GCA→ACA)	ygcU	putative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/10);  total (24/10)

GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCG  >  NC_000913/2898736‑2899026                                                                                                                                                  |                                                                                                                                                  ggggTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCa                                                                                                                                              <  1:342932/151‑1 (MQ=255)          ttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACg                                                                                                                                      >  2:285442/1‑150 (MQ=255)          ttAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACATTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACg                                                                                                                                      >  1:187671/1‑150 (MQ=255)           tAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGAc                                                                                                                                    >  1:143947/1‑151 (MQ=255)            aaaGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGAcc                                                                                                                                   >  1:36654/1‑151 (MQ=255)              aGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCat                                                                                                                                 <  1:591445/151‑1 (MQ=255)                aTTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCatat                                                                                                                               <  1:270326/151‑1 (MQ=255)                      aCTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCg                                                                                                                          >  2:186064/1‑150 (MQ=255)                              ttCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCg                                                                                                                 >  1:581711/1‑151 (MQ=255)                                  gTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGt                                                                                                                >  2:19107/1‑148 (MQ=255)                                    gATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAgt                                                                                                           >  2:130879/1‑151 (MQ=255)                                     atatAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAgtg                                                                                                          >  1:82165/1‑151 (MQ=255)                                       ataGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGtgtg                                                                                                        >  1:509422/1‑151 (MQ=255)                                              taTGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACt                                                                                                 >  1:127075/2‑151 (MQ=255)                                               aTGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTg                                                                                                >  2:442317/1‑151 (MQ=255)                                                                atgatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTg                                                                               <  2:512389/151‑1 (MQ=255)                                                                 tgatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTgcg                                                                                                                    <  2:79802/113‑1 (MQ=255)                                                                 tgatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTgcg                                                                                                                    >  1:79802/1‑113 (MQ=255)                                                                  gatgatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCt                                                                             >  1:242578/1‑151 (MQ=255)                                                                     gatgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTAc                                                                           <  2:360090/150‑1 (MQ=255)                                                                      atgTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTAcc                                                                          >  2:114501/1‑150 (MQ=255)                                                                        gtgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAgg                                                                       >  2:440564/1‑151 (MQ=255)                                                                             gAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGgccgcc                                                                  >  2:120394/1‑151 (MQ=255)                                                                                        ccAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTgcgc                                                         >  2:196283/1‑149 (MQ=255)                                                                                           gCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGGCGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCg                                                    >  2:513746/1‑151 (MQ=255)                                                                                                               tttAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATg                                 <  2:127078/150‑1 (MQ=255)                                                                                                               tttAATGCGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATg                                 <  2:5187/150‑1 (MQ=255)                                                                                                                      cGTGTGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCgtgg                         >  1:237209/1‑151 (MQ=255)                                                                                                                       gtgtGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCgtggt                        >  2:200346/1‑151 (MQ=255)                                                                                                                       gtgtGACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCgtggt                        >  1:275039/1‑151 (MQ=255)                                                                                                                         gtgACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGgtgt                      <  1:200345/151‑1 (MQ=255)                                                                                                                           gACGGTGCCATCAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTa                     >  1:8557/1‑150 (MQ=255)                                                                                                                                      cAGCCAATACTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACg         >  1:439232/1‑151 (MQ=255)                                                                                                                                              aCTGTTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAAcgcg  <  2:171928/150‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  GGGGTGAATTTAAAGATTTTCACTGTTACTTCAGTGATATAGCACAATGCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCG  >  NC_000913/2898736‑2899026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑