Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,912,885	A→G	I256T (ATC→ACC)	relA ←	(p)ppGpp synthetase I/GTP pyrophosphokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,912,885	0	A	G	100.0%	138.3 / NA	38	I256T (ATC→ACC)	relA	(p)ppGpp synthetase I/GTP pyrophosphokinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/20);  total (18/20)

GGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGT  >  NC_000913/2912748‑2913027                                                                                                                                          |                                                                                                                                               ggCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGgac                                                                                                                                    <  1:458377/150‑1 (MQ=255)     aTAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCa                                                                                                                               <  2:355579/151‑1 (MQ=255)       agtgagtgTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCAt                                                                                                                              <  2:177432/150‑1 (MQ=255)       agtgagtgTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATa                                                                                                                             <  2:225714/151‑1 (MQ=255)                           aGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCtt                                                                                                         >  2:161683/1‑151 (MQ=255)                                     aGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCAt                                                                                               >  1:262588/1‑151 (MQ=255)                                              gTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgc                                                                                       <  1:250246/150‑1 (MQ=255)                                              gTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgc                                                                                       <  1:28122/150‑1 (MQ=255)                                                aaaCGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAgcgcgc                                                                                     <  1:446409/150‑1 (MQ=255)                                                aaaCGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCTCa                                                                                    <  1:421017/151‑1 (MQ=255)                                                   cGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGAt                                                                                 <  2:216159/151‑1 (MQ=255)                                                    gCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATg                                                                                >  2:229426/1‑151 (MQ=255)                                                    gCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGGGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCACCGCGCAGATg                                                                                >  1:544236/1‑151 (MQ=255)                                                               aTACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAAct                                                                     >  1:470660/1‑151 (MQ=255)                                                                tACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAActc                                                                    >  2:98991/1‑151 (MQ=255)                                                                     aCCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCg                                                                >  1:340827/1‑150 (MQ=255)                                                                     aCCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCg                                                                >  2:384038/1‑150 (MQ=255)                                                                        gcacgcacATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGt                                                            <  2:351506/151‑1 (MQ=255)                                                                          acgcacATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAg                                                          <  2:38528/151‑1 (MQ=255)                                                                           cgcacATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAgt                                                         <  2:218320/151‑1 (MQ=255)                                                                                 tCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcgc                                                   >  1:164283/1‑151 (MQ=255)                                                                                 tCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcgc                                                   >  2:359723/1‑151 (MQ=255)                                                                                  cAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTcgc                                                   <  1:98989/150‑1 (MQ=255)                                                                                            atcaAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGc                                        <  2:417488/151‑1 (MQ=255)                                                                                                   gCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACGATACACTTCCGCTTTAACGCCTTCAGCTTTAATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCa                                  >  2:523232/1‑150 (MQ=255)                                                                                                      aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgca                              <  1:229426/151‑1 (MQ=255)                                                                                                      aGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgc                               >  2:78293/1‑150 (MQ=255)                                                                                                       ggTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgc                               >  1:274541/1‑149 (MQ=255)                                                                                                         ttCTTTTTCTGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATgcagc                            >  1:113971/1‑150 (MQ=255)                                                                                                                  tGCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCa                   >  2:466011/1‑150 (MQ=255)                                                                                                                   gCATTTTACGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCa                   <  2:323616/149‑1 (MQ=255)                                                                                                                          aCGCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGttt           <  2:365061/150‑1 (MQ=255)                                                                                                                            gCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGt         >  2:284622/1‑150 (MQ=255)                                                                                                                            gCCAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTa        <  2:475488/151‑1 (MQ=255)                                                                                                                              cAGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTAtt      <  1:424286/151‑1 (MQ=255)                                                                                                                               aGATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTc     >  2:151971/1‑151 (MQ=255)                                                                                                                                gATGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCg    <  1:276593/151‑1 (MQ=255)                                                                                                                                  tGCTGTAGGTGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGt  >  2:109562/1‑151 (MQ=255)                                                                                                                                          |                                                                                                                                               GGCGATAGTGAGTGTGCACTATCCCCAGTGCGGCATAGCAATCCTGTAAACGCTCGGCGACAATACGTACCGCACGCACATCAAACAGCTCATCAAAGGCGAGGTTCTTTTTCTGCATTTTACGCCAGATGCTGTAGATGTGTTTCGGACGACCATACACTTCCGCTTTAACGCCTTCAGCTTTCATCTCAGCGCGCAGATGACCAACGAACTCTTCGATGTAGTGTTCGCGGTCGAGACGCCGTTCATGCAGCAGTTTGGCAATTCGTTTGTATTCGGT  >  NC_000913/2912748‑2913027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑