Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,931,401	A→G	Q116R (CAG→CGG)	ygdG →	Ssb‑binding protein, misidentified as ExoIX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,931,401	0	A	G	100.0%	162.7 / NA	46	Q116R (CAG→CGG)	ygdG	Ssb‑binding protein, misidentified as ExoIX
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/27);  total (19/27)

AGCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACAGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTT  >  NC_000913/2931255‑2931548                                                                                                                                                   |                                                                                                                                                    aGCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGcc                                                                                                                                                 >  2:475480/1‑151 (MQ=255)   cGGGTCGACCGCCAAGGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCACCCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGc                                                                                                                                                  <  1:510503/148‑1 (MQ=255)            cGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGc                                                                                                                                      <  2:252012/151‑1 (MQ=255)              ccAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCaa                                                                                                                                    <  2:656527/151‑1 (MQ=255)                aaTGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACg                                                                                                                                  >  1:254512/1‑151 (MQ=255)                 aTGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACg                                                                                                                                  >  1:455553/1‑150 (MQ=255)                   gCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGAt                                                                                                                                <  2:167266/150‑1 (MQ=255)                   gCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCATGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGAtt                                                                                                                               <  2:245596/151‑1 (MQ=255)                     cGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGt                                                                                                                             >  1:206068/1‑151 (MQ=255)                      ggAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGtt                                                                                                                            <  1:460388/151‑1 (MQ=255)                         agagTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCg                                                                                                                         <  1:424504/151‑1 (MQ=255)                         agagTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCg                                                                                                                         <  1:485578/151‑1 (MQ=255)                                   gacgaGATGCCTGCATTACGCGCCGCCTTTGAGTAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATaa                                                                                                                 >  2:564088/1‑149 (MQ=255)                                    acgaGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAgg                                                                                                              <  2:244002/151‑1 (MQ=255)                                    acgaGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAgg                                                                                                              >  2:198449/1‑151 (MQ=255)                                     cgaGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGc                                                                                                             >  1:396433/1‑151 (MQ=255)                                       agaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCt                                                                                                            >  2:457670/1‑150 (MQ=255)                                       agaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTa                                                                                                           <  2:211794/151‑1 (MQ=255)                                       agaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTa                                                                                                           <  2:104891/151‑1 (MQ=255)                                        gaTGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTa                                                                                                           <  2:262889/150‑1 (MQ=255)                                            ccTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCACCGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGt                                                                                                       <  1:416946/150‑1 (MQ=255)                                                     cgcgCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACttt                                                                                             >  1:549145/1‑151 (MQ=255)                                                           gccTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCg                                                                                        >  1:571230/1‑150 (MQ=255)                                                               ttGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACa                                                                                     <  2:491468/149‑1 (MQ=255)                                                                  aGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACg                                                                                >  2:566328/1‑151 (MQ=255)                                                                    cAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGGCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACAtt                                                                                   >  1:658040/1‑146 (MQ=255)                                                                        gcggcgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCg                                                                          <  1:422334/151‑1 (MQ=255)                                                                          ggcgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACtt                                                                                              <  1:497519/129‑1 (MQ=255)                                                                          ggcgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACtt                                                                                              >  2:497521/1‑129 (MQ=255)                                                                            cgTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGAt                                                                      <  1:564083/151‑1 (MQ=255)                                                                                                  ggCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTgg                                                <  1:475477/151‑1 (MQ=255)                                                                                                         aaGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGcc                                         >  1:69137/1‑151 (MQ=255)                                                                                                           gCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCGCCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCAt                                       <  1:265292/151‑1 (MQ=255)                                                                                                             cGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCAttt                                     <  2:150008/151‑1 (MQ=255)                                                                                                              gATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCAttt                                     >  2:163873/1‑150 (MQ=255)                                                                                                              gATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTa                                    <  1:342919/151‑1 (MQ=255)                                                                                                               aTGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTAt                                   <  1:47690/151‑1 (MQ=255)                                                                                                                 gACTTAGCCGCCACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCg                                 <  2:69137/151‑1 (MQ=255)                                                                                                                       gccgccACGCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAg                           <  1:447869/151‑1 (MQ=255)                                                                                                                               gCTGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGc                   <  1:112845/151‑1 (MQ=255)                                                                                                                                 tGGCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGt                 >  1:431591/1‑151 (MQ=255)                                                                                                                                   gCGGTCAAAGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTTCTGTCAGTTACTTTCACCGCCATTACGTATTCGTGATTACTTCCAGAGACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGtt                <  1:489452/150‑1 (MQ=255)                                                                                                                                         aaaGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGa                                <  2:244143/128‑1 (MQ=255)                                                                                                                                         aaaGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGa                                >  1:244142/1‑128 (MQ=255)                                                                                                                                         aaaGTGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCgc         >  1:471791/1‑151 (MQ=255)                                                                                                                                             tGACACGGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCgcagc      >  2:520274/1‑150 (MQ=255)                                                                                                                                                cacgGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGtt  <  2:2386/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    AGCGGGTCGACCGCCAATGCCGGAAGAGTTGCACGACGAGATGCCTGCATTACGCGCCGCCTTTGAGCAACGCGGCGTCCCGTGCTGGTCAACCAGCGGCAACGAAGCCGATGACTTAGCCGCCACGCTGGCGGTCAAAGTGACACAGGCCGGGCATCAGGCAACGATTGTTTCGACAGATAAAGGCTACTGTCAGTTACTTTCACCGACATTACGTATTCGTGATTACTTCCAGAAACGTTGGCTGGATGCGCCATTTATCGATAAAGAATTTGGCGTTCAACCGCAGCAGTT  >  NC_000913/2931255‑2931548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑