Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,007,381	T→C	S374P (TCC→CCC)	ygeW →	putative carbamoyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,007,381	0	T	C	100.0%	150.0 / NA	41	S374P (TCC→CCC)	ygeW	putative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/20);  total (21/20)

CCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTG  >  NC_000913/3007235‑3007527                                                                                                                                                   |                                                                                                                                                   cccTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCg                                                                                                                                                <  1:344899/151‑1 (MQ=255)       aCATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCAAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGACGTATATCATCGCCGCGATGATCCTGCCCCGTAAAt                                                                                                                                           <  1:41479/150‑1 (MQ=255)         aTGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACg                                                                                                                                        >  2:498914/1‑151 (MQ=255)           gCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGc                                                                                                                                       <  1:575157/150‑1 (MQ=255)                    gccAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGt                                                                                                                             >  2:608727/1‑151 (MQ=255)                      cAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGc                                                                                                                           <  2:193371/151‑1 (MQ=255)                                  gcggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCa                                                                                                               <  1:362293/151‑1 (MQ=255)                                   cggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa                                                                                                              <  1:17674/151‑1 (MQ=255)                                   cggcgTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCaa                                                                                                              <  2:574529/151‑1 (MQ=255)                                       gTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgc                                                                                                          >  2:461310/1‑151 (MQ=255)                                       gTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgc                                                                                                          <  1:458572/151‑1 (MQ=255)                                          tCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAActgctg                                                                                                        >  1:584426/1‑150 (MQ=255)                                           ccTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaa                                                                                                      <  2:42543/151‑1 (MQ=255)                                            cTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGaa                                                                                                      >  2:469069/1‑150 (MQ=255)                                                 aagaagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc                                                                                                <  2:305806/151‑1 (MQ=255)                                                 aagaagGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAgc                                                                                                <  2:121180/151‑1 (MQ=255)                                                     agGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCaa                                                                                            <  1:206618/151‑1 (MQ=255)                                                     agGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCaa                                                                                            <  1:74609/151‑1 (MQ=255)                                                         gAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAc                                                                                        >  2:618807/1‑151 (MQ=255)                                                           aGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAAcgc                                                                                      >  1:279808/1‑151 (MQ=255)                                                              gACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTg                                                                                   <  2:388757/151‑1 (MQ=255)                                                                cTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGa                                                                                  >  2:85703/1‑150 (MQ=255)                                                                       cGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAAccc                                                                          >  1:392106/1‑151 (MQ=255)                                                                             cGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCgg                                                                     <  2:363878/150‑1 (MQ=255)                                                                                                   tacAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCctct                                              >  2:73910/1‑151 (MQ=255)                                                                                                             ccAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCag                                      >  2:282256/1‑149 (MQ=255)                                                                                                              cAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAgg                                   >  2:34369/1‑151 (MQ=255)                                                                                                                   ggAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCa                                <  2:455216/149‑1 (MQ=255)                                                                                                                    gAAGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCaaaa                             <  1:216287/151‑1 (MQ=255)                                                                                                                      aGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAagg                           >  1:510035/1‑151 (MQ=255)                                                                                                                      aGCCTTATATCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAag                            >  2:197575/1‑150 (MQ=255)                                                                                                                           tataTCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagg                      >  2:236641/1‑151 (MQ=255)                                                                                                                            atatCATCGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggatagg                      >  2:262686/1‑150 (MQ=255)                                                                                                                              atcatcGCCGCGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAaggataggat                    >  1:427543/1‑150 (MQ=255)                                                                                                                                    gccgcGATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCg             >  2:545741/1‑151 (MQ=255)                                                                                                                                        cgATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGtttt         <  1:294528/151‑1 (MQ=255)                                                                                                                                        cgATGATCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGtttt         <  2:161659/151‑1 (MQ=255)                                                                                                                                        cgATGAGCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCATCTGGTCGGCCCTCTATTGCCAGAGGCCAAAAGGATAGGATATGTCCGtttt         <  1:546682/151‑1 (MQ=255)                                                                                                                                         gatgatCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTtc        >  2:106548/1‑151 (MQ=255)                                                                                                                                              tCCTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAtt   >  1:654257/1‑151 (MQ=255)                                                                                                                                                cTGCCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCAATg  >  2:230090/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CCCTGTACATGCACTGCCTGCCAGCTGATATCAGCGGCGTATCCTGTAAAGAAGGTGAAGTGACTGAAGGCGTATTCGAAAAATACCGTATCGCTACCTACAAAGAAGCCAGCTGGAAGCCTTATATCATCGCCGCGATGATCCTGTCCCGTAAATACGCCAAACCAGGTGCACTGCTCGAGCAACTGCTGAAAGAAGCGCAAGAACGCGTGAAATAACCCCTCGGGCCGATCAGCTGGTCGGCCCTCTATTTCCAGAGGCCAAAAGGATAGGATATGTCCGTTTTCTCATTG  >  NC_000913/3007235‑3007527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑