Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,048,315	C→T	V243I (GTC→ATC)	gcvP ←	glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,048,315	0	C	T	100.0%	152.2 / NA	41	V243I (GTC→ATC)	gcvP	glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (22/19);  total (22/19)

AAAGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCA  >  NC_000913/3048181‑3048460                                                                                                                                       |                                                                                                                                                  aaaGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGAt                                                                                                                                   <  2:264174/151‑1 (MQ=255) aaaGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGAt                                                                                                                                   >  2:109566/1‑151 (MQ=255)  aaGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGAtt                                                                                                                                  >  1:89550/1‑151 (MQ=255)      aTGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTc                                                                                                                                >  2:166297/1‑149 (MQ=255)      aTGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTc                                                                                                                                >  1:509868/1‑149 (MQ=255)      aTGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTc                                                                                                                                >  2:490672/1‑149 (MQ=255)        gCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGtt                                                                                                                            <  1:58911/151‑1 (MQ=255)             gtgtGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCta                                                                                                                       <  2:528730/151‑1 (MQ=255)              tgtgGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCta                                                                                                                       >  1:158298/1‑150 (MQ=255)                  ggCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAg                                                                                                                  <  2:426068/151‑1 (MQ=255)                           aGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAgtgt                                                                                                          >  2:80171/1‑150 (MQ=255)                             cccATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGt                                                                                                       <  1:580804/151‑1 (MQ=255)                              ccATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTc                                                                                                      <  1:608976/151‑1 (MQ=255)                                   ggCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGaa                                                                                                 <  1:273086/151‑1 (MQ=255)                                       cGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTc                                                                                             >  1:3003/1‑151 (MQ=255)                                             aGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGt                                                                                       <  1:379707/151‑1 (MQ=255)                                                         aaCCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACctg                                                                           <  1:250690/151‑1 (MQ=255)                                                          aCCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACctgc                                                                          <  2:339377/151‑1 (MQ=255)                                                            cAAAAACAATATGCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACctgctg                                                                        >  1:224213/1‑151 (MQ=255)                                                               aaaCAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTa                                                                      >  2:331672/1‑150 (MQ=255)                                                                             cccTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGa                                                          >  1:371513/1‑148 (MQ=255)                                                                               cTGTTTACCCTGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCCGATCACAGTGTTGCGTGATTGCAGTTCGCTAATACGGGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGa                                                       >  1:59831/1‑149 (MQ=255)                                                                                    tACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCt                                                 <  2:269967/150‑1 (MQ=255)                                                                                    tACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTg                                                >  2:472230/1‑151 (MQ=255)                                                                                    tACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTg                                                >  2:175313/1‑151 (MQ=255)                                                                                      cccGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGAt                                              >  2:175154/1‑151 (MQ=255)                                                                                           cgcAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCg                                         <  2:645553/151‑1 (MQ=255)                                                                                            gcAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCg                                         >  1:115900/1‑150 (MQ=255)                                                                                               gTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCa                                     <  1:175312/151‑1 (MQ=255)                                                                                                   aCAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACttt                                 >  1:660130/1‑151 (MQ=255)                                                                                                    cAGCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACtttt                                <  1:112815/151‑1 (MQ=255)                                                                                                      gCACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTg                              >  1:57163/1‑151 (MQ=255)                                                                                                       cACCAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTgc                             <  1:72701/151‑1 (MQ=255)                                                                                                         ccAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTgcgc                           <  2:154822/151‑1 (MQ=255)                                                                                                         ccAGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTgcgc                           <  2:592127/151‑1 (MQ=255)                                                                                                           aGCGCCATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGcgcg                          >  1:75818/1‑150 (MQ=255)                                                                                                                cATAATATCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCg                    >  1:470992/1‑151 (MQ=255)                                                                                                                      atCGGCGGCAACGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGGAGTCGTGAATTTCACCAGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATc              >  1:385021/1‑151 (MQ=255)                                                                                                                               aaCGCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCaaa      >  1:64532/1‑150 (MQ=255)                                                                                                                                  gCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCa  <  2:307642/151‑1 (MQ=255)                                                                                                                                  gCTGATCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCa  <  2:624547/151‑1 (MQ=255)                                                                                                                                       |                                                                                                                                                  AAAGAATGCCGCGTGTGGGCCACCGTAGCCCATCGGCACGCCGAAGCGTTGCGCCGAACCAAAAACAATATCCGCGCCCTGTTTACCCGGCGCAGTTAACAGCACCAGCGCCATAATATCGGCGGCAACGCTGACCACAATTTTGCGTGATTTCAGTTCGCTAATAAGCGCAGTGTAGTCGTGAATTTCACCGGTAGTGCCTACCTGCTGTAACAGCACGCCGAAGACGTCCTGATGGTCGAGCACTTTTTGCGCGTCATCGACAATCACTTCAAAACCA  >  NC_000913/3048181‑3048460

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑