Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,614	T→C	R271R (CGT→CGC)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,614	0	T	C	100.0%	110.1 / NA	33	R271R (CGT→CGC)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/13);  total (20/13)

GCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTAC  >  NC_000913/3065467‑3065761                                                                                                                                                    |                                                                                                                                                    gCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTg                                                                                                                                                  >  2:340474/1‑151 (MQ=255)     gATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTggggg                                                                                                                                              >  2:337786/1‑151 (MQ=255)         aGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGaaa                                                                                                                                           >  1:457804/1‑150 (MQ=255)          gCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAAc                                                                                                                                         >  1:585395/1‑151 (MQ=255)                      gtgCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATtcct                                                                                                                             <  1:114481/151‑1 (MQ=255)                           agcagATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGtt                                                                                                                        <  1:308506/151‑1 (MQ=255)                                         aaCGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCgg                                                                                                          >  1:638270/1‑151 (MQ=255)                                              ggTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTg                                                                                                     >  2:322875/1‑151 (MQ=255)                                                    gTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTAc                                                                                                 >  2:470093/1‑149 (MQ=255)                                                       cTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGa                                                                                             >  2:85912/1‑150 (MQ=255)                                                         tATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGa                                                                                             <  1:66084/148‑1 (MQ=255)                                                         tATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAAt                                                                                           <  1:266819/150‑1 (MQ=255)                                                                ggAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGgtggtg                                                                                   >  2:167848/1‑151 (MQ=255)                                                                 gAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGc                                                                                  >  1:563037/1‑151 (MQ=255)                                                                  aaaTGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggtgg                                                                                     >  1:565863/1‑147 (MQ=255)                                                                      ggCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt                                                                              >  2:473788/1‑150 (MQ=255)                                                                                  tATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGc                                                                 >  2:566450/1‑151 (MQ=255)                                                                                   aTTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGc                                                                 <  2:482027/150‑1 (MQ=255)                                                                                     tCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCaa                                                               >  2:278926/1‑150 (MQ=255)                                                                                        gccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATc                                                           >  2:230824/1‑151 (MQ=255)                                                                                         ccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCa                                                          <  1:230824/151‑1 (MQ=255)                                                                                         ccgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCa                                                          <  1:624403/151‑1 (MQ=255)                                                                                          cgGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAg                                                         <  1:167699/151‑1 (MQ=255)                                                                                             aaTTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCgg                                                      >  2:367966/1‑151 (MQ=255)                                                                                                    gCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAgc                                               <  1:602539/151‑1 (MQ=255)                                                                                                      cGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAgcgc                                             >  2:12314/1‑151 (MQ=255)                                                                                                                tGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTg                                   >  1:587216/1‑151 (MQ=255)                                                                                                                     tGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACaa                               >  1:482394/1‑150 (MQ=255)                                                                                                                               cAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGAt                    <  1:220753/151‑1 (MQ=255)                                                                                                                               cAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGAt                    <  2:638280/151‑1 (MQ=255)                                                                                                                                      gCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTgc             <  1:30284/151‑1 (MQ=255)                                                                                                                                      gCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTgc             >  2:522298/1‑151 (MQ=255)                                                                                                                                           aaTGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCaa         <  1:85911/150‑1 (MQ=255)                                                                                                                                                 gcgcCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTAc  <  1:415348/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    GCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGATTTAC  >  NC_000913/3065467‑3065761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑