Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,266,688	C→T	A126A (GCG→GCA)	tdcA ←	tdc operon transcriptional activator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,266,688	0	C	T	100.0%	189.8 / NA	49	A126A (GCG→GCA)	tdcA	tdc operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/25);  total (24/25)

CAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGG  >  NC_000913/3266541‑3266832                                                                                                                                                    |                                                                                                                                                 cAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCt                                                                                                                                               >  1:243725/1‑151 (MQ=255)    cacaAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTc                                                                                                                                            <  1:500692/151‑1 (MQ=255)      caAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCgg                                                                                                                                          <  1:354383/151‑1 (MQ=255)        aaCTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAACCCTGTGCTTTCGGGa                                                                                                                                        <  1:236954/151‑1 (MQ=255)        aaCTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGa                                                                                                                                        <  2:325988/151‑1 (MQ=255)         aCTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGaa                                                                                                                                       <  2:242194/151‑1 (MQ=255)             ggACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACAcc                                                                                                                                   >  1:106708/1‑151 (MQ=255)                 tCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCtt                                                                                                                               >  1:216903/1‑151 (MQ=255)                    aaCAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTg                                                                                                                             >  1:578103/1‑150 (MQ=255)                           gCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACttgtt                                                                                                                     >  1:141785/1‑151 (MQ=255)                              cAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACttgttg                                                                                                                    <  2:7381/149‑1 (MQ=255)                                aCATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGatca                                                                                                                <  2:179648/151‑1 (MQ=255)                                      aaaTCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTg                                                                                                          <  1:207407/151‑1 (MQ=255)                                       aaTCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTg                                                                                                          >  2:576645/1‑150 (MQ=255)                                       aaTCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTg                                                                                                          >  2:344546/1‑150 (MQ=255)                                          ccTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACa                                                                                                       <  2:657206/150‑1 (MQ=255)                                              aaGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATaaaa                                                                                                  <  1:196596/151‑1 (MQ=255)                                              aaGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATaaaa                                                                                                  <  2:234189/151‑1 (MQ=255)                                                 cTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGt                                                                                                >  2:588534/1‑150 (MQ=255)                                                   tCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTaa                                                                                              >  1:584386/1‑150 (MQ=255)                                                     aTTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATa                                                                                                     <  1:364721/141‑1 (MQ=255)                                                     aTTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATa                                                                                                     >  2:364725/1‑141 (MQ=255)                                                        tCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACca                                                                                         >  2:453402/1‑150 (MQ=255)                                                          tGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACcaatc                                                                                      >  2:423308/1‑151 (MQ=255)                                                           gCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACcaat                                                                                       <  1:623364/149‑1 (MQ=255)                                                           gCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACcaat                                                                                       <  2:594598/149‑1 (MQ=255)                                                           gCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACcaat                                                                                       <  2:473541/149‑1 (MQ=255)                                                                tAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGa                                                                                 <  1:175974/150‑1 (MQ=255)                                                                tAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGa                                                                                 >  1:338978/1‑150 (MQ=255)                                                                 aaCGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGaa                                                                                <  2:655015/150‑1 (MQ=255)                                                                     tACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGaa                                                                            >  2:445041/1‑150 (MQ=255)                                                                      aCCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGaaa                                                                           >  2:60695/1‑150 (MQ=255)                                                                       ccAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAAc                                                                         >  1:219330/1‑151 (MQ=255)                                                                           tCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCaa                                                                      >  1:627595/1‑150 (MQ=255)                                                                                          gACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCcaccac                                                      >  2:189986/1‑151 (MQ=255)                                                                                            ccGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCg                                                    >  1:512746/1‑151 (MQ=255)                                                                                             cGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGc                                                   >  2:514829/1‑151 (MQ=255)                                                                                                cgcgGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCt                                                 <  1:576640/150‑1 (MQ=255)                                                                                                 gcgGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCa                                               <  2:216904/151‑1 (MQ=255)                                                                                                  cgGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCag                                              <  1:633876/151‑1 (MQ=255)                                                                                                     aTTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCaga                                             >  2:630602/1‑149 (MQ=255)                                                                                                        gCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGAc                                         >  2:292842/1‑150 (MQ=255)                                                                                                            gTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATAcc                                    <  2:518556/151‑1 (MQ=255)                                                                                                              aaGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAATTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAAGGAGACTTCCACCACCGCCTCAGAAGACATACCg                                   <  1:484354/150‑1 (MQ=255)                                                                                                               aGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCt                                 <  1:460312/151‑1 (MQ=255)                                                                                                                     aaGACAGTTGCGCTTCATACATAGAACCCTGTGCTTTCGGGAACACCTCTTGGAACTTGTTGATCATCCCTGACATAAAAGTAAACCCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTAtctc                           <  1:600146/151‑1 (MQ=255)                                                                                                                                   tCATACATAGAAACCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATAttt              <  2:120928/150‑1 (MQ=255)                                                                                                                                             aaaCCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACg   >  2:257743/1‑151 (MQ=255)                                                                                                                                              aaCCTGTGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACgg  >  2:190315/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CAGCACAAACTCGGACTCGAACAGCGGCTCAACATGTAAATCCTGAAGCTTCATTTCTGCACTTAACGTACCAATCGCAAAATCCAGTCGACCGTCGCGGATTGCCGGTAAGAACGAAGACAGTTGCGCTTCATACATAGAAACCTGCGCTTTCGGGAACACCTCTTTGAACTTGTTGATCATCCCTGACATAAAAGTAAAACCAATCAATGAAGGAAAACCAAATGAGACTTCCACCACCGCCTCAGAAGACATACCGCTTATCTCATTAACCATATTTTTCATTTCACGG  >  NC_000913/3266541‑3266832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑