Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,295,531	T→C	R46R (CGT→CGC)	yraN →	UPF0102 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,295,531	0	T	C	100.0%	109.9 / NA	32	R46R (CGT→CGC)	yraN	UPF0102 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/14);  total (18/14)

CTTATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGTGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCGCT  >  NC_000913/3295388‑3295673                                                                                                                                                |                                                                                                                                               cTTATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCgcggcggcg                                                                                                                                         >  1:323207/1‑151 (MQ=255)   tATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCgcggcggcg                                                                                                                                         >  2:552177/1‑149 (MQ=255)   tATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCga                                                                                                                                        <  1:287239/150‑1 (MQ=255)     tCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCgcggcggcg                                                                                                                                         >  2:398206/1‑147 (MQ=255)         ggCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAgatcgat                                                                                                                                 <  1:355132/151‑1 (MQ=255)           cTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCt                                                                                                                               >  2:222881/1‑151 (MQ=255)            tACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTg                                                                                                                              >  2:278175/1‑151 (MQ=255)             aCAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGa                                                                                                                             <  2:419248/151‑1 (MQ=255)               aGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGAt                                                                                                                            >  2:474430/1‑150 (MQ=255)                gTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATa                                                                                                                           >  1:78659/1‑150 (MQ=255)                     aacaaGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGt                                                                                                                     <  2:547508/151‑1 (MQ=255)                       caaGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTg                                                                                                                    >  2:201660/1‑150 (MQ=255)                                       ccGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCAtt                                                                                                   >  1:438215/1‑151 (MQ=255)                                                              ccGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATcgccg                                                                            <  1:147758/151‑1 (MQ=255)                                                               cGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATcgccgc                                                                           >  1:68489/1‑151 (MQ=255)                                                               cGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATcgccgc                                                                           >  1:184558/1‑151 (MQ=255)                                                                 gCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGctc                                                                         >  2:85350/1‑151 (MQ=255)                                                                   gATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTg                                                                       <  2:153870/151‑1 (MQ=255)                                                                      gCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTAt                                                                                 <  2:378276/138‑1 (MQ=255)                                                                      gCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTAt                                                                                 >  1:378272/1‑138 (MQ=255)                                                                      gCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTgcgc                                                                    >  1:315735/1‑151 (MQ=255)                                                                             aaGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATgg                                                             <  1:648216/151‑1 (MQ=255)                                                                                cacaAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATggcgg                                                          >  2:654970/1‑151 (MQ=255)                                                                                     gcgcGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGgcggcgcgg                                                     <  1:278175/151‑1 (MQ=255)                                                                                       gcgTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCa                                                   <  1:153848/151‑1 (MQ=255)                                                                                        cgTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGgcggcgccgc                                                    >  1:326041/1‑149 (MQ=255)                                                                                                    gAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTCAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCAtttt                                                                                                 <  1:429743/92‑1 (MQ=255)                                                                                                    gAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTCAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCAtttt                                                                                                 >  2:429748/1‑92 (MQ=255)                                                                                                         cAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCgcagc                                 >  2:449651/1‑151 (MQ=255)                                                                                                            aGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCaaa                              <  2:313299/151‑1 (MQ=255)                                                                                                                 tGCGGTTTATCGCCGCTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAaacaac                          >  1:147137/1‑150 (MQ=255)                                                                                                                            gccgcTAACGTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAAttatta               >  1:173546/1‑150 (MQ=255)                                                                                                                                     gTGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGccc     <  2:288566/151‑1 (MQ=255)                                                                                                                                      tGAACGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGGAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCg    >  1:360290/1‑151 (MQ=255)                                                                                                                                        aaCGAGCGCGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCGCt  <  1:222880/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               CTTATCATGGCTACAGTACCAACAAGGTCAGGTAGTCCCCGTCAGTTAACCACCAAACAGACCGGCGATGCGTGGGAAGCACAAGCGCGTCGCTGGCTGGAAGGCAAAGGACTGCGGTTTATCGCCGCTAACGTGAACGAGCGTGGCGGCGAGATCGATCTGATAATGCGTGAAGGCCGGACCACCATTTTTGTCGAGGTACGCTATCGCCGCTCTGCGCTTTATGGCGGCGCGGCAGCCAGTGTGACCCGCAGCAAACAACACAAATTATTACAGACTGCCCGCT  >  NC_000913/3295388‑3295673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑