Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,311,285	T→C	intergenic (‑117/+130)	pnp ← / ← rpsO	polynucleotide phosphorylase/polyadenylase/30S ribosomal subunit protein S15

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,311,285	0	T	C	100.0%	145.2 / NA	38	intergenic (‑117/+130)	pnp/rpsO	polynucleotide phosphorylase/polyadenylase/30S ribosomal subunit protein S15
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/19);  total (19/19)

ACTGGAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCC  >  NC_000913/3311138‑3311430                                                                                                                                                    |                                                                                                                                                  aCTGGAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCccct                                                                                                                                                 <  2:184796/150‑1 (MQ=255)     gAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTa                                                                                                                                            <  1:661480/151‑1 (MQ=255)      aaTTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAg                                                                                                                                           <  1:508178/151‑1 (MQ=255)        tttACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGcc                                                                                                                                         >  2:7534/1‑151 (MQ=255)                     ggATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTg                                                                                                                            <  2:111229/151‑1 (MQ=255)                         tAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCaa                                                                                                                         <  1:383151/150‑1 (MQ=255)                          aaGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACg                                                                                                                       <  1:613936/151‑1 (MQ=255)                           aGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACgg                                                                                                                      <  2:598175/151‑1 (MQ=255)                             cAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGaa                                                                                                                    <  1:544491/151‑1 (MQ=255)                               aTGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGa                                                                                                                  <  1:500500/151‑1 (MQ=255)                                tGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGa                                                                                                                     >  2:572095/1‑147 (MQ=255)                                tGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCCCATCCTCGCGACTAATGACACCCCTAACCCAGCACTATGTGGGGAAAGCCCCTCAGTAGCCGCGCGAACCACTGCAACGGAAg                                                                                                                   <  1:10331/149‑1 (MQ=255)                                 gTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtc                                                                                                                <  1:342217/151‑1 (MQ=255)                                   aaTATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtcat                                                                                                              >  1:527853/1‑151 (MQ=255)                                     tatCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtcattc                                                                                                            >  2:378330/1‑151 (MQ=255)                                       tCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGAtcattc                                                                                                            >  2:243388/1‑149 (MQ=255)                                          tttctctttctTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGc                                                                                                       >  1:141910/1‑151 (MQ=255)                                            tctctttctTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGca                                                                                                      <  2:87531/150‑1 (MQ=255)                                                 ttcttAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAAt                                                                                                 >  2:226017/1‑150 (MQ=255)                                                    ttAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACAt                                                                                             >  2:143116/1‑151 (MQ=255)                                                        aCAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGt                                                                                         <  1:584904/151‑1 (MQ=255)                                                         cAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGtt                                                                                        <  1:34192/151‑1 (MQ=255)                                                               cTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGt                                                                                  >  2:357357/1‑151 (MQ=255)                                                                                aaTACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGc                                                                 <  2:636042/151‑1 (MQ=255)                                                                                    cccGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTg                                                              >  1:69447/1‑150 (MQ=255)                                                                                           ttctGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAgg                                                      >  1:313014/1‑151 (MQ=255)                                                                                            tctGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGaaaa                                                          <  2:535023/146‑1 (MQ=255)                                                                                                 gcATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCa                                                 >  1:657337/1‑150 (MQ=255)                                                                                                  cATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACt                                               >  1:66152/1‑151 (MQ=255)                                                                                                         gcgACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGccc                                        >  1:273484/1‑151 (MQ=255)                                                                                                            aCTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCt                                     <  2:66737/151‑1 (MQ=255)                                                                                                               aaTGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCtttt                                  <  1:288294/151‑1 (MQ=255)                                                                                                                 tGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCt                                >  1:283118/1‑151 (MQ=255)                                                                                                                              ccAGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGa                   <  2:624028/151‑1 (MQ=255)                                                                                                                                aGCTCTATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAAt                 >  2:92937/1‑151 (MQ=255)                                                                                                                                  ctctATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAAtt                >  2:565537/1‑150 (MQ=255)                                                                                                                                   tctATGTGGGTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAAtt                >  1:472174/1‑149 (MQ=255)                                                                                                                                            gTAAAGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGa     <  2:344417/151‑1 (MQ=255)                                                                                                                                               aaGCCCCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGAccc  >  2:543054/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                  ACTGGAATTTACGAACGATCGGATTAAGCAATGTAATATCCTTTCTCTTTCTTAGACAGTACCTTACGGCACTGGTGTTAATACCCGATCTTCTGCGCATCCTCGCGACTAATGACAACCCTAACCCAGCTCTATGTGGGTAAAGCCTCTCATTAGCCGCGCGAACCTCTGCAACGGAAGATCATTCATAGCAACAATACATTAGTTTCCAGTGAATTGCTGCCGTCAGCTTGAAAAAAGGGGCCACTCAGGCCCCCTTTTCTGAAACTCGCAAGAATTAGCGACGCAGACCC  >  NC_000913/3311138‑3311430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑