Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,511,488	A→G	L286P (CTC→CCC)	yhfZ ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,511,488	0	A	G	100.0%	97.9 / NA	29	L286P (CTC→CCC)	yhfZ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/11);  total (18/11)

ACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG  >  NC_000913/3511340‑3511630                                                                                                                                                     |                                                                                                                                               aCGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATgggcgggc                                                                                                                                              <  1:393967/151‑1 (MQ=255)              gCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTa                                                                                                                                  <  2:668851/150‑1 (MQ=255)                         ttGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGc                                                                                                                       >  2:95037/1‑150 (MQ=255)                                 gTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACgcagc                                                                                                                >  2:322039/1‑149 (MQ=255)                                       aTCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGttgttg                                                                                                        <  2:125414/151‑1 (MQ=255)                                        tCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGc                                                                                                       >  1:581754/1‑151 (MQ=255)                                           aTAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCAtt                                                                                                    <  2:607601/151‑1 (MQ=255)                                              aCGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTggg                                                                                                 <  2:329791/151‑1 (MQ=255)                                                gcctgcctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTGTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTa                                                                                               <  2:581759/151‑1 (MQ=255)                                                   tgcctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAatc                                                                                            >  1:218495/1‑151 (MQ=255)                                                   tgcctCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAAATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAatc                                                                                            >  2:654352/1‑151 (MQ=255)                                                          cAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACt                                                                                     >  1:279337/1‑151 (MQ=255)                                                          cAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACt                                                                                     >  2:123862/1‑151 (MQ=255)                                                                aGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTc                                                                               >  2:613619/1‑151 (MQ=255)                                                                   ttGAGACTGGTTTCCATATCAGATGACTTTAGTTTAATTACTTGGAAGAGGATGCGTACTCAATACCCGAGGGAGATGGGCGGGCAGGGCGTGTGTAGCTACAACGGCACGCAGCAGTTGTTGCATTGGGAAATCATCGACTCGCGTCAg                                                                             >  2:63986/1‑150 (MQ=255)                                                                         cTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACg                                                                      >  2:393811/1‑151 (MQ=255)                                                                            gTTTCCATATCCGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCt                                                                   >  1:479275/1‑151 (MQ=255)                                                                                    atCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTgg                                                            <  2:131563/150‑1 (MQ=255)                                                                                    atCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGc                                                           >  2:491747/1‑151 (MQ=255)                                                                                     tCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGcc                                                          >  1:537041/1‑151 (MQ=255)                                                                                      cAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGcc                                                          <  2:360353/150‑1 (MQ=255)                                                                                                  gTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATcgcg                                             >  2:50100/1‑151 (MQ=255)                                                                                                        taaCTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGt                                       >  1:403686/1‑151 (MQ=255)                                                                                                         aaCTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTc                                      >  2:194430/1‑151 (MQ=255)                                                                                                             cGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCtgt                                   <  2:256469/150‑1 (MQ=255)                                                                                                                   cTGTTCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAgcgg                             >  1:233475/1‑150 (MQ=255)                                                                                                                       tCCCCACTCACTACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCg                        >  1:459965/1‑151 (MQ=255)                                                                                                                               cactACCCGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTaa                 <  1:440601/150‑1 (MQ=255)                                                                                                                                      cGTTGTTGATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCa         <  2:479737/151‑1 (MQ=255)                                                                                                                                             gATGGGCGGGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAg  <  2:448876/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                               ACGTTGACGACCTGCATCATGCCTTTGCAGATGTCCTTATCAATAACGCCTGCCTCGCAAAGCAGGTTGAGTCTGGTTTCCATATCAGATGCCTTTCGTTTAATAACTCGGTTCCTGTTCCCCACTCACTACCCGTTGTTGATGGGCGAGCAGGGCGTGTTTATCTACAACGGCACGCAGCAGTTGTTGCATTGGGTAATCATCGACTCGCGTCAGGACCACGGCTTCGGTGGCCTGTAAAAATCGCGGATCGTCTGTGAGCGGCGTCGCCTCTAATCCCAGCATGGTCAG  >  NC_000913/3511340‑3511630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑