Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,524,787	T→C	A639A (GCT→GCC)	mrcA →	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,524,787	0	T	C	100.0%	107.0 / NA	30	A639A (GCT→GCC)	mrcA	penicillin‑binding protein 1a, murein transglycosylase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/14);  total (16/14)

ATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGCTTTG  >  NC_000913/3524644‑3524931                                                                                                                                                |                                                                                                                                                 aTCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTcc                                                                                                                                            <  1:223813/150‑1 (MQ=255)      cAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGag                                                                                                                                      <  1:63435/151‑1 (MQ=255)                           cggcgTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTAcc                                                                                                                  >  2:623133/1‑150 (MQ=255)                            ggcgTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCa                                                                                                                 >  2:565579/1‑150 (MQ=255)                             gcgTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAAt                                                                                                               >  1:167223/1‑151 (MQ=255)                               gTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGc                                                                                                             >  2:374221/1‑151 (MQ=255)                                 gATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCg                                                                                                           <  1:242041/151‑1 (MQ=255)                                  aTTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGc                                                                                                          <  1:624601/151‑1 (MQ=255)                                      tCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCt                                                                                                      >  2:60299/1‑151 (MQ=255)                                         aaGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGa                                                                                                   <  1:565574/151‑1 (MQ=255)                                                  cGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCaaa                                                                                          <  1:179221/151‑1 (MQ=255)                                                           ccTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGtt                                                                                 <  2:495884/151‑1 (MQ=255)                                                              gCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGt                                                                              <  1:392696/151‑1 (MQ=255)                                                                       gCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGa                                                                      <  1:231058/150‑1 (MQ=255)                                                                          atatTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACt                                                                    >  1:647049/1‑149 (MQ=255)                                                                            atTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGgcg                                                                >  2:516273/1‑151 (MQ=255)                                                                              tCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGgcgc                                                               <  2:127559/150‑1 (MQ=255)                                                                                      tttACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGt                                                       >  1:115484/1‑150 (MQ=255)                                                                                       ttACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTa                                                      >  2:105085/1‑150 (MQ=255)                                                                                        tACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTAcg                                                    >  2:545325/1‑151 (MQ=255)                                                                                        tACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTAc                                                     <  2:597797/150‑1 (MQ=255)                                                                                              gATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTAcgcaccg                                               <  2:466881/150‑1 (MQ=255)                                                                                                           cGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAacac                                 >  2:153862/1‑151 (MQ=255)                                                                                                           cGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAacac                                 >  2:611615/1‑151 (MQ=255)                                                                                                             aaCGTGCTGGAAAATAACGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTc                               <  1:168457/151‑1 (MQ=255)                                                                                                                            aaCGATGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTg                 >  1:204682/1‑150 (MQ=255)                                                                                                                                 tGTTGAAGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTa             >  2:387231/1‑149 (MQ=255)                                                                                                                                      aaGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGc      >  1:79468/1‑151 (MQ=255)                                                                                                                                      aaGATGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGc      <  2:603693/151‑1 (MQ=255)                                                                                                                                          tGTCGCCATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGCTTTg  >  1:407878/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                 ATCAGCAAAATTGAAAACGATCAGGGCGGCGTGATTTTCGAAGCGAAACCGAAAGTAGCCTGCCCGGAATGCGATATTCCGGTGATTTACGGTGATACGCAGAAATCGAACGTGCTGGAAAATAACGATGTTGAAGATGTCGCTATCTCCCGCGAGCAGCAGAATGTTTCTGTACCAATGCCGCAGCTGGAGCAGGCAAATCAGGCGTTAGTGGCGAAGACTGGCGCGCAGGAGTACGCACCGCACGTCATCAACACTCCGCTGGCATTCCTGATTAAGAGTGCTTTG  >  NC_000913/3524644‑3524931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑