Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,552,089	T→C	T129A (ACT→GCT)	malP ←	maltodextrin phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,552,089	0	T	C	97.1%	120.6 / NA	35	T129A (ACT→GCT)	malP	maltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/16);  minor base A (1/0);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

TGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATCCTGTACATCCTGATACCAGCCGAGATTCA  >  NC_000913/3551949‑3552235                                                                                                                                             |                                                                                                                                                   tGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTTTGACCGTAACCCGTCGCAGACTGACCGACAGATGCCAtt                                                                                                                                             >  1:245734/1‑148 (MQ=255)  gCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAg                                                                                                                                          <  2:571743/150‑1 (MQ=255)   cTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGt                                                                                                                                         >  1:561049/1‑150 (MQ=255)    ttCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCg                                                                                                                                       >  1:334508/1‑151 (MQ=255)     tCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCACCCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTATCCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCg                                                                                                                                       <  1:281132/150‑1 (MQ=255)          gtgGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGaa                                                                                                                                  <  1:39904/150‑1 (MQ=255)                     aCGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACgccgcc                                                                                                                        >  2:570891/1‑149 (MQ=255)                       gggTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGAc                                                                                                                    >  2:192138/1‑151 (MQ=255)                                cTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGac                                                                                                           >  1:465146/1‑151 (MQ=255)                                           gTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTAccc                                                                                                <  1:225657/151‑1 (MQ=255)                                              aTCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAgc                                                                                             <  2:191705/151‑1 (MQ=255)                                                ccGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAgcg                                                                                            <  1:246753/150‑1 (MQ=255)                                                    cgcTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCggg                                                                                       >  1:387989/1‑151 (MQ=255)                                                    cgcTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCggg                                                                                       <  2:627261/151‑1 (MQ=255)                                                                   gCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCttcttc                                                                         <  1:325609/150‑1 (MQ=255)                                                                    ccATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTcc                                                                        >  2:320151/1‑150 (MQ=255)                                                                        cGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCcagc                                                                     >  1:132675/1‑149 (MQ=255)                                                                          aCAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGt                                                                 <  2:349325/151‑1 (MQ=255)                                                                            aaaaGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTcc                                                               <  2:78061/151‑1 (MQ=255)                                                                             aaaGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCg                                                              <  2:470496/151‑1 (MQ=255)                                                                               aGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTc                                                            <  2:465150/151‑1 (MQ=255)                                                                                  cTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTc                                                            >  2:316186/1‑148 (MQ=255)                                                                                  cTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTc                                                            >  2:625115/1‑148 (MQ=255)                                                                                    ggCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGAtt                                                       >  2:479145/1‑151 (MQ=255)                                                                                       ggAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGAt                                                    >  2:319042/1‑151 (MQ=255)                                                                                               ccATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATa                                              >  2:317628/1‑149 (MQ=255)                                                                                                    ttGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTc                                       >  1:369244/1‑151 (MQ=255)                                                                                                         aGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACg                                   >  2:638107/1‑150 (MQ=255)                                                                                                         aGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACg                                   <  2:523702/150‑1 (MQ=255)                                                                                                             cAGACCGTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATcc                              >  1:182137/1‑151 (MQ=255)                                                                                                                   gTAACCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATCCTGTAc                         >  1:279708/1‑150 (MQ=255)                                                                                                                     aaCCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATCCTGTACATc                      >  2:89200/1‑151 (MQ=255)                                                                                                                      aCCCGTCGCAGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATCCTGTACATcc                     <  2:394266/151‑1 (MQ=255)                                                                                                                               aGACTGACCGACAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATCCTGTACATCCTGATACCAg            <  2:393000/151‑1 (MQ=255)                                                                                                                                         aCAGCTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATCCTGTACATCCTGATACCAGCCGAGATTCa  <  2:403361/151‑1 (MQ=255)                                                                                                                                             |                                                                                                                                                   TGCTTCGTTGTGGCGGAACCACGGGTAGTTACTGCGATGCCAGTCATCCGGCGCTTCAACCTGTTTGCCATCGACAAAAGACTGGCGGAACAAACCATATTGATAGTTCAGACCGTAACCCGTCGCAGACTGACCGACAGTTGCCATTGAGTCGAGGAAGCACGCCGCCAGACGTCCCAGACCACCGTTACCCAGCGCCGGGTCGATCTCTTCTTCCAGCAGGTCCGTCAGATTGATGTCATAAGCCTTCAACGAATCCTGTACATCCTGATACCAGCCGAGATTCA  >  NC_000913/3551949‑3552235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑