Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,575,617	G→A	A183T (GCA→ACA)	yhgN →	UPF0056 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,575,617	0	G	A	100.0%	190.2 / NA	50	A183T (GCA→ACA)	yhgN	UPF0056 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/25);  total (25/25)

CAGATGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGGCAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCAC  >  NC_000913/3575470‑3575761                                                                                                                                                    |                                                                                                                                                 cAGATGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACa                                                                                                                                                <  1:206183/150‑1 (MQ=255)    aTGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            <  2:585401/151‑1 (MQ=255)      ggggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCa                                                                                                                                           <  2:29937/150‑1 (MQ=255)      ggggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAg                                                                                                                                          <  1:165077/151‑1 (MQ=255)       gggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            >  2:173624/1‑148 (MQ=255)       gggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            >  2:670271/1‑148 (MQ=255)       gggCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAAccc                                                                                                                                            >  2:59488/1‑148 (MQ=255)          cATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGAt                                                                                                                                        <  2:442167/149‑1 (MQ=255)           aTCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGtt                                                                                                                                     >  1:565639/1‑151 (MQ=255)             cTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTcc                                                                                                                                   >  1:357014/1‑151 (MQ=255)               ggTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTcc                                                                                                                                   <  1:460340/149‑1 (MQ=255)               ggTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTc                                                                                                                                 >  2:84412/1‑151 (MQ=255)                 tGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCg                                                                                                                                >  1:526145/1‑150 (MQ=255)                           gctgctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGa                                                                                                                     <  1:135064/151‑1 (MQ=255)                            ctgctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGa                                                                                                                     >  2:425495/1‑150 (MQ=255)                              gctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAAt                                                                                                                   <  1:220562/150‑1 (MQ=255)                               ctgGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAAtgt                                                                                                                 >  2:95816/1‑151 (MQ=255)                                      ggggCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATg                                                                                                            <  1:610420/149‑1 (MQ=255)                                       gggCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAg                                                                                                         <  2:597198/151‑1 (MQ=255)                                                            gCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGttttt                                                                                     <  1:460113/150‑1 (MQ=255)                                                              tACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCg                                                                                   >  2:664210/1‑150 (MQ=255)                                                               aCAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCg                                                                                   <  1:578880/149‑1 (MQ=255)                                                                      tCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGaa                                                                            <  1:551049/149‑1 (MQ=255)                                                                       cGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAAccc                                                                         <  1:282706/151‑1 (MQ=255)                                                                         cTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCgg                                                                       >  2:419277/1‑151 (MQ=255)                                                                                tACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTc                                                                >  2:352623/1‑151 (MQ=255)                                                                                 aCGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTca                                                               <  1:59489/151‑1 (MQ=255)                                                                                 aCGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTc                                                                >  2:362217/1‑150 (MQ=255)                                                                                     ctgctgGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAAc                                                           >  2:27347/1‑151 (MQ=255)                                                                                        ctgGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTAc                                                        >  1:264156/1‑151 (MQ=255)                                                                                          gggCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCg                                                      <  1:664199/151‑1 (MQ=255)                                                                                            gCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCggg                                                    >  2:363324/1‑151 (MQ=255)                                                                                             cGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGc                                                   <  1:425490/151‑1 (MQ=255)                                                                                              gagaAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCg                                                  <  1:629014/151‑1 (MQ=255)                                                                                                  aaGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACGACCGGGCGAACa                                              <  2:24372/151‑1 (MQ=255)                                                                                                      ggTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACt                                          <  2:163537/151‑1 (MQ=255)                                                                                                      ggTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACt                                          <  1:73761/151‑1 (MQ=255)                                                                                                       gTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACtt                                         <  2:280181/151‑1 (MQ=255)                                                                                                               aCTTGAACGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAAc                                 >  2:331502/1‑151 (MQ=255)                                                                                                                    aaCGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTg                            >  2:195633/1‑151 (MQ=255)                                                                                                                      cGCCTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTgg                           >  1:349684/1‑150 (MQ=255)                                                                                                                        ccTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAAc                        <  2:575232/151‑1 (MQ=255)                                                                                                                        ccTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAAc                        <  1:37722/151‑1 (MQ=255)                                                                                                                         cTGATGGGATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACg                       >  2:587453/1‑151 (MQ=255)                                                                                                                              gggATTGATTCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAatc                  >  2:458656/1‑151 (MQ=255)                                                                                                                                gattgattCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAatcat                >  1:550578/1‑151 (MQ=255)                                                                                                                                       tCTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAAc          <  1:60870/150‑1 (MQ=255)                                                                                                                                        cTGGTGATGATGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACg         >  2:156140/1‑150 (MQ=255)                                                                                                                                             gatgatGACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGc    >  1:114121/1‑150 (MQ=255)                                                                                                                                              atgatgACAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCAc  >  2:464387/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CAGATGGGGCATCTGGTGATTGCTCTGCTGCTGGCCTGGGGCGGCACCTTTGTCATCCTGCTACAGTCTTCGCTATTTTTACGTCTGCTGGGCGAGAAAGGGGTGAACGCACTTGAACGCCTGATGGGATTGATTCTGGTGATGATGGCAACCCAGATGTTCCTCGACGGCATTCGAATGTGGATGAAGGGGTAACGAGGAGAGTTTTTCGAAAAGAACCCGGTAGCGTCACAACTACCGGGCGAACAAACTTAACTCAACAGCTGGAACGCAATCATCCCAACGATGGCAC  >  NC_000913/3575470‑3575761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑