Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 310,993 A→G V115A (GTA→GCA)  ecpR ← putative transcriptional regulator for the ecp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913310,9930AG97.8% 160.0 / NA 45V115A (GTA→GCA) ecpRputative transcriptional regulator for the ecp operon
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (20/24);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACT  >  NC_000913/310846‑311138
                                                                                                                                                   |                                                                                                                                                 
gATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTCATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGcc                                                                                                                                                 >  1:26964/1‑150 (MQ=255)
   ttAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACg                                                                                                                                              <  2:422229/150‑1 (MQ=255)
   ttAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGc                                                                                                                                             >  1:515075/1‑151 (MQ=255)
   ttAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGc                                                                                                                                             >  1:415541/1‑151 (MQ=255)
         tGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTa                                                                                                                                        <  1:166284/150‑1 (MQ=255)
              tCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATAtt                                                                                                                                   <  1:605858/150‑1 (MQ=255)
              tCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTGCTTAATATGTTTTCTCAGAAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATAtt                                                                                                                                   <  1:126435/150‑1 (MQ=255)
                ccTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCt                                                                                                                                >  1:393324/1‑151 (MQ=255)
                   gggCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTa                                                                                                                               <  2:575591/149‑1 (MQ=255)
                           aTGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATAcc                                                                                                                     <  1:543231/151‑1 (MQ=255)
                             gCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCa                                                                                                                    >  1:519026/1‑150 (MQ=255)
                               ggATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCaat                                                                                                                  >  1:447785/1‑150 (MQ=255)
                                      ttccatttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCt                                                                                                          >  1:227252/1‑151 (MQ=255)
                                        ccatttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTa                                                                                                         >  1:543111/1‑150 (MQ=255)
                                          atttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAgg                                                                                                      <  2:447790/151‑1 (MQ=255)
                                           tttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAgg                                                                                                      <  1:300359/150‑1 (MQ=255)
                                           tttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGc                                                                                                     >  1:531113/1‑151 (MQ=255)
                                            ttccCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGcc                                                                                                    >  1:498766/1‑151 (MQ=255)
                                                       tGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCt                                                                                         >  2:117764/1‑151 (MQ=255)
                                                       tGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCt                                                                                         >  1:8346/1‑151 (MQ=255)
                                                                  ttcttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGcaatc                                                                               <  1:349358/150‑1 (MQ=255)
                                                                  ttcttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGcaa                                                                                 <  2:84890/148‑1 (MQ=255)
                                                                    cttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGcaatcaat                                                                            >  2:344300/1‑151 (MQ=255)
                                                                     ttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGcaatcaat                                                                            >  1:587382/1‑150 (MQ=255)
                                                                     ttAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGcaatcaat                                                                            >  1:330425/1‑150 (MQ=255)
                                                                      tAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATa                                                                           <  2:330428/150‑1 (MQ=255)
                                                                       aaTATCTTCTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATCATTTGTTAAGGCCTCTGACTTTCTGGCCGcaatcaat                                                                            >  1:453232/1‑148 (MQ=255)
                                                                                   cAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTcc                                                               <  1:647427/149‑1 (MQ=255)
                                                                                      gAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCttt                                                          <  2:209733/151‑1 (MQ=255)
                                                                                          cTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACcg                                                      >  2:187087/1‑151 (MQ=255)
                                                                                                  aaTCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATaa                                               <  1:282812/150‑1 (MQ=255)
                                                                                                   aTCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAAcc                                             <  2:502207/151‑1 (MQ=255)
                                                                                                       caTAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACcattc                                         <  2:311277/151‑1 (MQ=255)
                                                                                                        aTAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATTCCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACcattca                                        <  2:511899/151‑1 (MQ=255)
                                                                                                        aTAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACcattc                                         >  1:285941/1‑150 (MQ=255)
                                                                                                            aCCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTAt                                    <  2:133433/151‑1 (MQ=255)
                                                                                                                  tCTTTTCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACgg                               <  2:316039/150‑1 (MQ=255)
                                                                                                                       tCTAATATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTtat                          <  1:186465/150‑1 (MQ=255)
                                                                                                                        cTAATATCACTACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTtata                         >  2:162053/1‑150 (MQ=255)
                                                                                                                          aaTATCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAAt                      >  2:49179/1‑151 (MQ=255)
                                                                                                                             atCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCa                   <  2:352085/151‑1 (MQ=255)
                                                                                                                             atCACGACTCAGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCa                   <  1:414335/151‑1 (MQ=255)
                                                                                                                                       aGTCCAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAAt         <  2:8345/151‑1 (MQ=255)
                                                                                                                                           cAGCGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGt     <  2:272157/151‑1 (MQ=255)
                                                                                                                                              cGTATGCCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACt  <  1:416685/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GATTTAGGTTGCATTCCCTGGGCCGTCATGCGGATAATTTCCATTTCCCGGTCCGTGATTTTATCTTTCTTAATATCTTTTCTCAGGAACCTGCCATTAATCACATAGACCAGTTCTTTTCTAATATCACGACTCAGTCCAGCGTATACCACGCCCCTAATATTGCTATTGTAATACCAATAATTTGCTAAGGCCTCTGACTTTCTGGCCGCAATCAATACGACCTGTTTCCCCTTTACCGCGGATAACCATTCATTATCACGGTTTATAAATTCAGATAAAGAATCCGTACT  >  NC_000913/310846‑311138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑