Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,717,476 T→C V56A (GTA→GCA)  ghrB → glyoxylate/hydroxypyruvate reductase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,717,4760TC97.5% 144.3 / ‑3.7 40V56A (GTA→GCA) ghrBglyoxylate/hydroxypyruvate reductase B
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (18/21);  total (19/21)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

AAAGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTATTAACAGAAACCGTCGCC  >  NC_000913/3717334‑3717621
                                                                                                                                              |                                                                                                                                                 
aaaGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCg                                                                                                                                           <  2:583225/151‑1 (MQ=255)
 aaGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGc                                                                                                                                          <  2:315596/151‑1 (MQ=255)
  aGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCa                                                                                                                                         <  1:528378/151‑1 (MQ=255)
   gCCTTACCTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCAt                                                                                                                                        >  1:250350/1‑151 (MQ=255)
         ccTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTgg                                                                                                                                  <  2:287459/151‑1 (MQ=255)
           tgatgaTTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGa                                                                                                                                 >  1:97765/1‑150 (MQ=255)
            gatgatTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGaaa                                                                                                                               <  2:343319/151‑1 (MQ=255)
                            gCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCg                                                                                                               >  2:37588/1‑151 (MQ=255)
                             ccTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGt                                                                                                              <  2:307003/151‑1 (MQ=255)
                             ccTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGt                                                                                                              <  2:532212/151‑1 (MQ=255)
                               tGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGa                                                                                                                      <  1:305068/141‑1 (MQ=255)
                               tGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGa                                                                                                                      >  2:305070/1‑141 (MQ=255)
                                 cAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGcc                                                                                                           >  1:164114/1‑150 (MQ=255)
                                   agagCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCcac                                                                                                         >  1:263631/1‑150 (MQ=255)
                                     agCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATc                                                                                                      >  2:57669/1‑151 (MQ=255)
                                       cATTTCACCGTTCACCAGGTGGCATACCTCAGCCCACAAACCGTCGAACAAAACGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATc                                                                                                      >  1:565705/1‑149 (MQ=255)
                                           tCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGa                                                                                                 <  1:405548/150‑1 (MQ=255)
                                                gTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCg                                                                                           >  1:440626/1‑151 (MQ=255)
                                                  tCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGt                                                                                          <  2:190405/150‑1 (MQ=255)
                                                      cAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCt                                                                                     >  1:590960/1‑151 (MQ=255)
                                                                 ccTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAAtttt                                                                          >  1:639396/1‑151 (MQ=255)
                                                                   tCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTg                                                                         >  1:618465/1‑150 (MQ=255)
                                                                            aaaCCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATgc                                                               <  1:334444/151‑1 (MQ=255)
                                                                                   cGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTAc                                                         <  1:546994/150‑1 (MQ=255)
                                                                                   cGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCGCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTAc                                                         <  1:235601/150‑1 (MQ=255)
                                                                                     aaCAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGc                                                      <  2:402350/151‑1 (MQ=255)
                                                                                        aaaaTGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCg                                                   <  2:129576/151‑1 (MQ=255)
                                                                                           aTGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGaaa                                                <  2:177773/151‑1 (MQ=255)
                                                                                            tGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGaaa                                                >  1:630902/1‑150 (MQ=255)
                                                                                               agcaATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAAtt                                            >  1:182300/1‑151 (MQ=255)
                                                                                                gcaATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTc                                           >  2:414612/1‑151 (MQ=255)
                                                                                                     ttttGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTctgct                                       <  2:114106/150‑1 (MQ=255)
                                                                                                                    aGGTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAAcc                       <  2:473664/151‑1 (MQ=255)
                                                                                                                      gTTTACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGt                     <  2:519386/151‑1 (MQ=255)
                                                                                                                        ttACTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGACAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTAt                   >  2:539739/1‑151 (MQ=255)
                                                                                                                          aCTGGGTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTAtt                  <  1:522909/150‑1 (MQ=255)
                                                                                                                               gTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGaaa                                                >  2:523130/1‑115 (MQ=255)
                                                                                                                               gTTCAAACGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGaaa                                                <  1:523127/115‑1 (MQ=255)
                                                                                                                                    aaCGAGAATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTATTAACAGAAACCg       >  2:145023/1‑151 (MQ=255)
                                                                                                                                         gaATGCAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTATTAACAGAAACCGTCGcc  >  1:628940/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                 
AAAGCCTTACCTGATGATTTACTGCAACGCCTGCAAGAGCATTTCACCGTTCACCAGGTGGCAAACCTCAGCCCACAAACCGTCGAACAAAATGCAGCAATTTTTGCCGAAGCTGAAGGTTTACTGGGTTCAAACGAGAATGTAAATGCCGCATTGCTGGAAAAAATGCCGAAACTGCGTGCCACATCAACGATCTCCGTCGGCTATGACAATTTTGATGTCGATGCGCTTACCGCCCGAAAAATTCTGCTGATGCACACGCCAACCGTATTAACAGAAACCGTCGCC  >  NC_000913/3717334‑3717621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑