Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,790,211	T→C	intergenic (‑130/+109)	waaH ← / ← tdh	LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,790,211	0	T	C	100.0%	148.6 / NA	39	intergenic (‑130/+109)	waaH/tdh	LPS(HepIII)‑glucuronic acid glycosyltransferase/L‑threonine 3‑dehydrogenase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/26);  total (13/26)

GGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCG  >  NC_000913/3790067‑3790357                                                                                                                                                 |                                                                                                                                                   ggTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATaa                                                                                                                                              <  2:264086/151‑1 (MQ=255)    gCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAagag                                                                                                                                           <  2:318835/151‑1 (MQ=255)    gCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAaga                                                                                                                                            <  1:285331/150‑1 (MQ=255)         tcatcaTAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCa                                                                                                                                       <  2:492061/150‑1 (MQ=255)            tcatAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGcac                                                                                                                                   >  2:474522/1‑151 (MQ=255)             catAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGcaca                                                                                                                                  <  1:358176/151‑1 (MQ=255)             catAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGcac                                                                                                                                   <  2:184621/150‑1 (MQ=255)                 aaTTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAAtt                                                                                                                               >  2:132414/1‑150 (MQ=255)                 aaTTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTa                                                                                                                              <  1:93592/151‑1 (MQ=255)                   ttACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACg                                                                                                                            >  2:146778/1‑151 (MQ=255)                         acgacCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAActc                                                                                                                       <  2:618479/150‑1 (MQ=255)                                   aaGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACt                                                                                                            <  1:151534/151‑1 (MQ=255)                                       tATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGAc                                                                                                         >  2:632747/1‑150 (MQ=255)                                        aTCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACa                                                                                                        <  2:37383/150‑1 (MQ=255)                                        aTCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACa                                                                                                        <  1:629801/150‑1 (MQ=255)                                        aTCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAg                                                                                                       <  2:644678/151‑1 (MQ=255)                                            tGACGTTTTACAGGCCTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGAtt                                                                                                   >  1:335210/1‑151 (MQ=255)                                                gTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTAc                                                                                               >  2:232493/1‑151 (MQ=255)                                                       aGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTttatt                                                                                                                 >  1:467500/1‑126 (MQ=255)                                                       aGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTttatt                                                                                                                 <  2:467504/126‑1 (MQ=255)                                                               caTTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTa                                                                                <  2:309641/150‑1 (MQ=255)                                                                aTTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTa                                                                                <  2:667535/150‑1 (MQ=255)                                                                  tAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATcc                                                                             >  2:109665/1‑151 (MQ=255)                                                                   aaGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCt                                                                            <  1:405896/151‑1 (MQ=255)                                                                       aaCTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGa                                                                        <  1:115372/151‑1 (MQ=255)                                                                               aGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAggg                                                                <  1:624795/151‑1 (MQ=255)                                                                               aGCCTGTGAGATATTATTTTTACAGGCGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAggg                                                                >  1:262528/1‑151 (MQ=255)                                                                                 ccTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCt                                                              <  1:427078/151‑1 (MQ=255)                                                                                      gagaTATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAAt                                                         <  2:375744/151‑1 (MQ=255)                                                                                        gaTATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATAc                                                       <  1:461413/151‑1 (MQ=255)                                                                                                    aCAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTc                                           <  2:20833/151‑1 (MQ=255)                                                                                                           agagagTCACTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTaa                                    <  1:663476/151‑1 (MQ=255)                                                                                                                    cTTCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTc                           >  1:164870/1‑151 (MQ=255)                                                                                                                      tCAGGGTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTc                           <  2:126343/149‑1 (MQ=255)                                                                                                                           gTAGAGAGATAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATaa                    <  2:159265/151‑1 (MQ=255)                                                                                                                               agagaTAATGAGAGCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACttt                >  1:151678/1‑151 (MQ=255)                                                                                                                                         agagCAGCCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTgg      >  1:666046/1‑151 (MQ=255)                                                                                                                                           agcagcCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGcc    <  2:501047/151‑1 (MQ=255)                                                                                                                                             cagcCATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCg  >  1:263155/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                   GGTGCTGTTCATCATAAATTACCGACGACCTCTGAAGTTATCCTGACGTTTTACAGGCTTTGTATTAAGAAACTATTAAGCCTGTGAGATATTATTTTTACAGGTGAGAGAGTCACTTCAGGGTAGAGAGATAATGAGAGCAGCTATATAAGAGATCAGCACAATTACGAACTCTTTATTACACTGACAGGATTTTACATATTTAACAGATTATCCTCAGATAAAAGGGCTGGAATACCAGCCCTTGTTCGTGTTAATCCCAGCTCAGAATAACTTTCCCGGACTGGCCCG  >  NC_000913/3790067‑3790357

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑