Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	329,853	A→G	T131A (ACG→GCG)	betT →	choline transporter of high affinity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	329,853	0	A	G	100.0%	122.3 / NA	34	T131A (ACG→GCG)	betT	choline transporter of high affinity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/19);  total (15/19)

CCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTG  >  NC_000913/329712‑329996                                                                                                                                              |                                                                                                                                                ccAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAgg                                                                                                                                        >  2:296620/1‑151 (MQ=255)  cataaCAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGc                                                                                                                                       >  2:520615/4‑151 (MQ=255)       cAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCcgcgc                                                                                                                                  <  2:392491/151‑1 (MQ=255)              aaCCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGc                                                                                                                           >  2:650761/1‑151 (MQ=255)               aCCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCg                                                                                                                          <  1:436619/151‑1 (MQ=255)                  ggAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATg                                                                                                                       <  2:627609/151‑1 (MQ=255)                  ggAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATg                                                                                                                       <  2:487982/151‑1 (MQ=255)                                             gATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCtt                                                                                             >  1:96128/1‑150 (MQ=255)                                                          gcCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCCCTACGGCTTAACCGGCTGGTCg                                                                                <  1:454838/150‑1 (MQ=255)                                                                  cGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATgcg                                                                       >  2:6284/1‑151 (MQ=255)                                                                           ccTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGc                                                              <  2:179340/151‑1 (MQ=255)                                                                                tGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGgc                                                         >  1:481237/1‑151 (MQ=255)                                                                                 gTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGgcg                                                        <  1:37552/151‑1 (MQ=255)                                                                                        tcCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGa                                                  >  2:448605/1‑150 (MQ=255)                                                                                         ccGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATa                                                <  2:618280/151‑1 (MQ=255)                                                                                          cGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATAc                                               <  2:187998/151‑1 (MQ=255)                                                                                           gTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACt                                              <  1:493469/151‑1 (MQ=255)                                                                                               ccGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAg                                          <  1:514423/151‑1 (MQ=255)                                                                                               ccGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAg                                          <  1:662330/151‑1 (MQ=255)                                                                                                 gAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCt                                        <  1:564825/151‑1 (MQ=255)                                                                                                    ccGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATc                                     >  1:532650/1‑151 (MQ=255)                                                                                                        tAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGTCAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGtt                                  >  1:656451/1‑150 (MQ=255)                                                                                                          aCGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTtata                               <  1:262704/151‑1 (MQ=255)                                                                                                          aCGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTtat                                >  1:641580/1‑150 (MQ=255)                                                                                                            gCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAt                             <  2:581176/151‑1 (MQ=255)                                                                                                             cAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAtt                            <  1:111162/151‑1 (MQ=255)                                                                                                             cAGTATATGCAGCCGCCGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAAtt                            <  1:500512/151‑1 (MQ=255)                                                                                                                        gccgccGGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCAcc                 <  1:336512/151‑1 (MQ=255)                                                                                                                         ccgccgGAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCAcc                 >  1:47692/1‑150 (MQ=255)                                                                                                                              ggAAGGCGCGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGc           >  2:457815/1‑151 (MQ=255)                                                                                                                                   gcgcGGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGgc      >  1:394606/1‑151 (MQ=255)                                                                                                                                     gcgGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGgcgc    >  2:647463/1‑151 (MQ=255)                                                                                                                                      cgGGACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCt   <  2:146762/151‑1 (MQ=255)                                                                                                                                       gggACAGGCGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTg  >  2:573045/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                                CCAGAACAATCCAAACCGGAATTCAGCCTGCTGAGTTGGGCGGCGATGCTGTTTGCTGCCGGGATCGGTATCGACCTGATGTTCTTCTCCGTAGCCGAACCGGTAACGCAGTATATGCAGCCGCCGGAAGGCGCGGGACAGACGATTGAGGCCGCGCGTCAGGCGATGGTCTGGACGCTGTTTCACTACGGCTTAACCGGCTGGTCGATGTATGCGCTGATGGGCATGGCGCTCGGATACTTTAGCTATCGTTATAATTTGCCGCTCACCATCCGCTCGGCGCTG  >  NC_000913/329712‑329996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑