Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,828,150	G→A	G172G (GGC→GGT)	gltS ←	glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,828,150	0	G	A	100.0%	106.5 / NA	29	G172G (GGC→GGT)	gltS	glutamate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/14);  total (15/14)

ATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCA  >  NC_000913/3828004‑3828292                                                                                                                                                   |                                                                                                                                               aTAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCAccc                                                                                                                                             >  2:323966/1‑150 (MQ=255) aTAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCa                                                                                                                                            <  2:666529/151‑1 (MQ=255)       tCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCAcc                                                                                                                                       >  1:641322/1‑150 (MQ=255)                aCCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACg                                                                                                                             >  2:521143/1‑151 (MQ=255)                     cGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGca                                                                                                                        <  2:298791/151‑1 (MQ=255)                           gATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGcc                                                                                                                  <  2:262437/151‑1 (MQ=255)                              cATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCAt                                                                                                                <  2:293296/150‑1 (MQ=255)                                  cGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGcc                                                                                                            >  1:297593/1‑150 (MQ=255)                                  cGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTAGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGcc                                                                                                            >  2:384789/1‑150 (MQ=255)                                     cccACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACt                                                                                                         <  2:497626/150‑1 (MQ=255)                                            ccGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCg                                                                                                  <  2:288397/150‑1 (MQ=255)                                                  tttCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTgg                                                                                            <  1:520010/150‑1 (MQ=255)                                                          gCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCAt                                                                                   <  2:467889/151‑1 (MQ=255)                                                            cGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATa                                                                                  >  2:323878/1‑150 (MQ=255)                                                               cGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACg                                                                               <  2:144533/150‑1 (MQ=255)                                                                      tCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATg                                                                        <  1:567349/150‑1 (MQ=255)                                                                                     aTACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACg                                                        <  1:323876/151‑1 (MQ=255)                                                                                          gTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAgcg                                                   >  1:186363/1‑151 (MQ=255)                                                                                                 gtggtgGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTAc                                            >  2:52261/1‑151 (MQ=255)                                                                                                                  cAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGaa                              >  2:360271/1‑148 (MQ=255)                                                                                                                  cAGATAGCGCGCCACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAaga                           <  2:376162/151‑1 (MQ=255)                                                                                                                              cACCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCg               >  2:490875/1‑151 (MQ=255)                                                                                                                               aCCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCgg              >  2:514169/1‑151 (MQ=255)                                                                                                                               aCCGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCg               >  1:527283/1‑150 (MQ=255)                                                                                                                                 cGGACCGCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGcc            <  2:216620/151‑1 (MQ=255)                                                                                                                                       gCCAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGc      >  2:576113/1‑151 (MQ=255)                                                                                                                                         cAATCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGccc    >  2:470947/1‑151 (MQ=255)                                                                                                                                           aTCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGcccc   >  2:54237/1‑150 (MQ=255)                                                                                                                                           aTCAAGCCACCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCa  <  1:388158/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                               ATAGTTTCAATCAGCACCAACGAGGTGATCATGCGTCCCACATCCGGCTTTTCAAACGCCGTCGGGACTTCCTGGTCATCCGGAATACCGTTCGGCGTGGTGGAGTGTTTCACCAGATAGCGCGCCACCGGACCGCCAATCAAGCCGCCCAGCACCAGACCGAACGTTGCACAGGCCATCGCCACTTCCGTCGCATTGGTGAAGCCATAACGTTCAATGAACAATTTACTCCACGCAGCGCCCGTACCGTGACCGCCGGAAAGAGTAATAGAACCGGCCAACAGCCCCA  >  NC_000913/3828004‑3828292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑