Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,916,435 G→A A314V (GCG→GTG)  atpD ← F1 sector of membrane‑bound ATP synthase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,916,4350GA100.0% 152.5 / NA 40A314V (GCG→GTG) atpDF1 sector of membrane‑bound ATP synthase, beta subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/25);  total (15/25)

ACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCGCAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGTCGGCTGATAACCTA  >  NC_000913/3916289‑3916579
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aCTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACaa                                                                                                                                               >  1:619065/1‑150 (MQ=255)
        ccACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTg                                                                                                                                      <  2:612574/151‑1 (MQ=255)
              gcgGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCgg                                                                                                                                  >  1:441605/1‑149 (MQ=255)
               cgGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCgg                                                                                                                                  >  1:612040/1‑148 (MQ=255)
                         gtgtCCCGGACCACCCCCCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATCCCCAGAGACGCGATCTGACGGCTCAGTACCCCGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGgtcagtc                                                                                                                     <  1:651793/151‑1 (MQ=255)
                           gtTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGgtcagtca                                                                                                                    <  2:173148/150‑1 (MQ=255)
                                 gACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCAt                                                                                                              <  2:29554/150‑1 (MQ=255)
                                     aaccaCCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCa                                                                                                         <  2:584573/151‑1 (MQ=255)
                                      accaccAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAg                                                                                                        <  1:352200/151‑1 (MQ=255)
                                         accaGCGGGTCCAGCTGACGGCTGGGGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAgg                                                                                                       >  1:105387/1‑149 (MQ=255)
                                            aGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTACCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGt                                                                                                  <  1:582560/151‑1 (MQ=255)
                                                     aGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCt                                                                                         >  1:533592/1‑151 (MQ=255)
                                                     aGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCt                                                                                         <  1:437840/151‑1 (MQ=255)
                                                      gCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTg                                                                                        >  1:308216/1‑151 (MQ=255)
                                                           cGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACgg                                                                                   <  2:563202/151‑1 (MQ=255)
                                                            ggCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTg                                                                                            >  1:613206/1‑141 (MQ=255)
                                                            ggCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTg                                                                                            <  2:613217/141‑1 (MQ=255)
                                                                       tCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAAc                                                                       >  1:213429/1‑151 (MQ=255)
                                                                          aGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAg                                                                    <  1:453810/151‑1 (MQ=255)
                                                                              ggTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGtttt                                                                <  1:419807/151‑1 (MQ=255)
                                                                                  aaCGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTtggtg                                                            >  2:421729/1‑151 (MQ=255)
                                                                                  aaCGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTtggtg                                                            <  2:415126/151‑1 (MQ=255)
                                                                                     ggCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAg                                                         <  2:564542/151‑1 (MQ=255)
                                                                                      gCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAgg                                                        >  2:628722/1‑151 (MQ=255)
                                                                                          ggTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGAt                                                    <  1:37777/151‑1 (MQ=255)
                                                                                             aGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATAc                                                  <  2:271039/150‑1 (MQ=255)
                                                                                                         aCGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAAc                                     <  2:612051/151‑1 (MQ=255)
                                                                                                                  gACGGCTCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCAtctc                            >  2:326933/1‑151 (MQ=255)
                                                                                                                        tCAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGc                      >  2:667902/1‑151 (MQ=255)
                                                                                                                         cAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGc                      <  2:448689/150‑1 (MQ=255)
                                                                                                                         cAGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGc                      <  1:572260/150‑1 (MQ=255)
                                                                                                                          aGTACCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCa                    <  2:213430/151‑1 (MQ=255)
                                                                                                                            tACCACGGGTGCGTCAAGGTGCACAAAGGGGGTTGCCGAAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAgg                  >  1:598304/1‑151 (MQ=255)
                                                                                                                             aCCACGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAggg                 >  2:23077/1‑151 (MQ=255)
                                                                                                                                aCGGTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGTCg              <  2:600843/151‑1 (MQ=255)
                                                                                                                                   gTTGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGTCGGCt           <  1:288603/151‑1 (MQ=255)
                                                                                                                                     tGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGt                >  2:522110/1‑144 (MQ=255)
                                                                                                                                     tGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGt                <  1:522107/144‑1 (MQ=255)
                                                                                                                                     tGCGTCAAGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGTCGGCTg          <  1:668369/150‑1 (MQ=255)
                                                                                                                                            aGGTGCACAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGTCGGCTGATAACCTa  <  1:142528/151‑1 (MQ=255)
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ACTGAACGCCACGCGCGGTGTCGTAGTGTTCCTGACCAACCACCAGCGGGTCCAGCTGACGGCTGGTGGAGTCCAGCGGGTCAACGGCCGGGTAGATACCCAGAGACGCGATCTGACGGCTCAGTACCACGGTTGCGTCAAGGTGCGCAAAGGTGGTTGCCGGAGACGGGTCAGTCAAGTCATCCGCAGGTACGTATACTGCCTGTACGGAGGTGATAGAACCAGTTTTGGTGGAGGTGATACGTTCCTGCAGAACGCCCATCTCTTCCGCCAGGGTCGGCTGATAACCTA  >  NC_000913/3916289‑3916579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑