Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,943,370	C→T	intergenic (+21/‑65)	rrsC → / → gltU	16S ribosomal RNA of rrnC operon/tRNA‑Glu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,943,370	0	C	T	100.0%	75.4 / NA	40	intergenic (+21/‑65)	rrsC/gltU	16S ribosomal RNA of rrnC operon/tRNA‑Glu
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/20);  total (20/20)

AGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTT  >  NC_000913/3943248‑3943513                                                                                                                           |                                                                                                                                                agCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGa                                                                                                                      >  2:7183/1‑150 (MQ=14)      aaCCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGa                                                                                                                 <  2:296282/150‑1 (MQ=14)       aCCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGaa                                                                                                                >  1:662731/1‑150 (MQ=14)           tCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAg                                                                                                             <  1:165125/149‑1 (MQ=14)             gggAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAg                                                                                                         <  1:658358/151‑1 (MQ=14)                aGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCg                                                                                                      <  2:81730/151‑1 (MQ=14)                aGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCg                                                                                                      >  2:115806/1‑151 (MQ=14)                   gcgcTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCt                                                                                                   >  1:338198/1‑151 (MQ=14)                     gcTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTg                                                                                                 <  2:136964/151‑1 (MQ=14)                         aCCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGa                                                                                              >  2:557685/1‑150 (MQ=14)                            aCTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGc                                                                                           >  2:387797/1‑150 (MQ=14)                            aCTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCa                                                                                          <  1:248060/151‑1 (MQ=14)                               ttGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAg                                                                                         <  1:126512/149‑1 (MQ=14)                                 gtgATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTg                                                                                     <  1:216958/151‑1 (MQ=14)                                      tCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATAc                                                                                 <  2:99356/150‑1 (MQ=14)                                       cATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGt                                                                               >  1:641662/1‑151 (MQ=14)                                          gACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTccc                                                                            <  2:557779/151‑1 (MQ=14)                                             tGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCtt                                                                         >  1:342723/1‑151 (MQ=14)                                                   gAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCt                                                                    >  1:654658/1‑150 (MQ=14)                                                    aaGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTag                                                                  <  2:210783/151‑1 (MQ=14)                                                     aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTaga                                                                 <  2:16377/151‑1 (MQ=14)                                                     aGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTaga                                                                 <  1:266748/151‑1 (MQ=14)                                                         gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGcc                                                             >  2:544279/1‑151 (MQ=14)                                                         gTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGcc                                                             <  1:7184/151‑1 (MQ=14)                                                           aaCAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGccc                                                            >  2:243769/1‑150 (MQ=14)                                                             cAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAgg                                                         >  2:304703/1‑151 (MQ=14)                                                               aGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGac                                                       <  1:154926/151‑1 (MQ=14)                                                                     ccGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGcc                                                 >  2:382076/1‑151 (MQ=14)                                                                     ccGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGcc                                                 >  1:485151/1‑151 (MQ=14)                                                                                 cTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCAcggcgg                                     >  2:598400/1‑151 (MQ=14)                                                                                       ttGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGGAACa                                >  2:103171/1‑150 (MQ=14)                                                                                              aCCTCCTTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCg                        <  1:598394/151‑1 (MQ=14)                                                                                               cctcctTACCTTAAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGa                       <  1:521847/151‑1 (MQ=14)                                                                                                        cttaAAGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGAATcccc                 >  1:127106/1‑148 (MQ=14)                                                                                                           aaaGAAGCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAggg            <  1:267701/150‑1 (MQ=14)                                                                                                            aagaagCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGa          <  1:397264/151‑1 (MQ=14)                                                                                                            aagaagCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGGTCGGATCCCCTAgggg           >  2:187986/1‑150 (MQ=9)                                                                                                                agCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGcc      >  2:264025/1‑151 (MQ=14)                                                                                                                 gCGTTCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGcc      >  1:452090/1‑150 (MQ=14)                                                                                                                     tCTTTGTAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACtt  <  1:307397/150‑1 (MQ=14)                                                                                                                           |                                                                                                                                                AGCTTAACCTTCGGGAGGGCGCTTACCACTTTGTGATTCATGACTGGGGTGAAGTCGTAACAAGGTAACCGTAGGGGAACCTGCGGTTGGATCACCTCCTTACCTTAAAGAAGCGTTCTTTGCAGTGCTCACACAGATTGTCTGATAGGAAGTGAAAAGCAAGGCGTCTTGCGAAGCAGACTGATACGTCCCCTTCGTCTAGAGGCCCAGGACACCGCCCTTTCACGGCGGTAACAGGGGTTCGAATCCCCTAGGGGACGCCACTT  >  NC_000913/3943248‑3943513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑