Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,949,005	T→C	Q322R (CAG→CGG)	yifB ←	magnesium chelatase family protein and putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,949,005	0	T	C	100.0%	104.7 / NA	30	Q322R (CAG→CGG)	yifB	magnesium chelatase family protein and putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/16);  total (14/16)

GCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCTGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTT  >  NC_000913/3948859‑3949145                                                                                                                                                   |                                                                                                                                             gCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGccc                                                                                                                                          <  2:645538/151‑1 (MQ=255)             aaCGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCgg                                                                                                                              >  1:546390/1‑151 (MQ=255)              aCGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGc                                                                                                                             >  1:492607/1‑151 (MQ=255)               cGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGct                                                                                                                            >  2:129678/1‑151 (MQ=255)                       cTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCa                                                                                                                      >  2:372708/1‑149 (MQ=255)                              ggCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATc                                                                                                               <  1:129674/149‑1 (MQ=255)                              ggCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCa                                                                                                             <  1:109862/151‑1 (MQ=255)                                cGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCACCCAgt                                                                                                           <  2:470886/151‑1 (MQ=255)                                       cGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGcc                                                                                                    <  1:103520/151‑1 (MQ=255)                                         gTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGt                                                                                                  >  1:427948/1‑151 (MQ=255)                                               gTTTCCCTGATAATGTCCGGTAGGGTTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAAt                                                                                            <  2:385488/151‑1 (MQ=255)                                                       gATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGt                                                                                      <  2:234681/149‑1 (MQ=255)                                                        aTAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGc                                                                                   >  2:75406/1‑151 (MQ=255)                                                           aTGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCtca                                                                                <  2:48268/151‑1 (MQ=255)                                                              tCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCtcatc                                                                              >  1:136121/1‑150 (MQ=255)                                                                  gTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGaa                                                                         <  1:383575/151‑1 (MQ=255)                                                                      ggCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAg                                                                     <  2:580962/151‑1 (MQ=255)                                                                       gCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGc                                                                    >  1:82565/1‑151 (MQ=255)                                                                               tCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGt                                                             <  2:80433/150‑1 (MQ=255)                                                                                          acaaGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCg                                                 >  2:584993/1‑151 (MQ=255)                                                                                            aaGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGa                                                <  1:122252/150‑1 (MQ=255)                                                                                              gCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAAt                                             <  2:73989/151‑1 (MQ=255)                                                                                                     aCGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCg                                      >  1:98531/1‑151 (MQ=255)                                                                                                      cGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCgg                                     >  2:220096/1‑151 (MQ=255)                                                                                                      cGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCgg                                     <  2:641133/151‑1 (MQ=255)                                                                                                                 ggTTATTTTTGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCGGGCCCTGGAAtt                          >  1:85447/1‑151 (MQ=255)                                                                                                                       ttttGCTCGTGTGCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCGGGCCCTGGAATTGCGcc                     >  2:509487/1‑150 (MQ=255)                                                                                                                                tgtgCGTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCGGGCCCTGGAATTGCGCCACCGCCt              <  1:509122/148‑1 (MQ=255)                                                                                                                                     gTGAAAGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGc      <  2:14209/151‑1 (MQ=255)                                                                                                                                         aaGATGGATCCGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGtt  >  2:623852/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                             GCCGGTTGAGATAACGTAATGTCTGTTCTGGCGTGCAGCGGTTATGGTTTCCCTGATAATGTCCGGTAGGGCTGGGATTCATCGCCGCAACAAGCTGGAAACGGGCTGGATAGGTTATTTTTGCTCGTGTGCGTGAAAGATGGATCTGCCCGGATTCAATCGGCTCTCGCAAGGCATCCAGTGTACGCCGTTCAAATTCAGGTAGCTCATCAAGAAAAAGCACGCCGTTATGCGCCAGCGAAATTTCACCGGGCCCTGGAATTGCGCCACCGCCTACCATCGCAGTT  >  NC_000913/3948859‑3949145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑