Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,017,296	T→C	intergenic (+104/‑37)	udp → / → rmuC	uridine phosphorylase/DNA recombination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,017,296	0	T	C	100.0%	132.5 / NA	34	intergenic (+104/‑37)	udp/rmuC	uridine phosphorylase/DNA recombination protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/13);  total (21/13)

GTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGA  >  NC_000913/4017154‑4017442                                                                                                                                               |                                                                                                                                                   gTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTtgtg                                                                                                                                            <  2:187900/151‑1 (MQ=255)    aaaaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCgg                                                                                                                                         >  1:544287/1‑151 (MQ=255)     aaaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCggg                                                                                                                                        >  1:549438/1‑151 (MQ=255)      aaTCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGt                                                                                                                                       >  1:253137/1‑151 (MQ=255)                       cgcgTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCagg                                                                                                                       >  1:106938/1‑150 (MQ=255)                              tcTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtg                                                                                                               >  1:490013/1‑151 (MQ=255)                                tgctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgtg                                                                                                             >  1:165901/1‑151 (MQ=255)                                 gctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgtg                                                                                                             <  1:470786/150‑1 (MQ=255)                                 gctgTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTtgtg                                                                                                             <  1:552038/150‑1 (MQ=255)                                              tctcCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCAt                                                                                               >  2:407923/1‑151 (MQ=255)                                                    gTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGttt                                                                                          >  1:426240/1‑150 (MQ=255)                                                                  gcgTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGt                                                                           <  2:165903/151‑1 (MQ=255)                                                                   cgTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGtt                                                                          >  2:123666/1‑151 (MQ=255)                                                                            ttttGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGgtgt                                                                 >  2:566012/1‑151 (MQ=255)                                                                              ttGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTgg                                                               >  2:388147/1‑151 (MQ=255)                                                                                 tATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCa                                                             >  1:558121/1‑150 (MQ=255)                                                                                    ttttGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATtg                                                         >  2:460491/1‑151 (MQ=255)                                                                                       tGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATtggct                                                      >  1:589520/1‑151 (MQ=255)                                                                                         cGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATtggctgg                                                    >  2:423499/1‑151 (MQ=255)                                                                                         cGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATtggctgg                                                    >  2:349836/1‑151 (MQ=255)                                                                                                   cGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGcc                                           >  2:232839/1‑150 (MQ=255)                                                                                                        gAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTAt                                     <  2:129065/151‑1 (MQ=255)                                                                                                                     aaCTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCgcagc                        >  2:77590/1‑151 (MQ=255)                                                                                                                       cTGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCgcagca                       <  1:487209/150‑1 (MQ=255)                                                                                                                        tGGACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCaa                      >  1:154352/1‑150 (MQ=255)                                                                                                                         ggACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCaaa                     <  2:641010/150‑1 (MQ=255)                                                                                                                         ggACGTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCaa                      <  2:106941/149‑1 (MQ=255)                                                                                                                             gTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGcc                <  2:394249/151‑1 (MQ=255)                                                                                                                             gTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGcc                >  1:534877/1‑151 (MQ=255)                                                                                                                             gTTTGTACAGCACAATTCCATTTTGTGCGGGTAAGTTGTTGAGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGcc                >  1:13097/1‑151 (MQ=255)                                                                                                                                      gCACAATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTa       <  2:228565/151‑1 (MQ=255)                                                                                                                                       cacaATTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAg      <  1:323884/151‑1 (MQ=255)                                                                                                                                          aaTTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTg   <  2:31055/151‑1 (MQ=255)                                                                                                                                           aTTCCATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGa  <  1:596870/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                   GTGAAAATCGTGGTGGAAGCGGCGCGTCGTCTGCTGTAATTCTCTTCTCCTGTCTGAAGGCCGACGCGTTCGGCCTTTTGTATTTTTGCGTAGCGCCTCGCAGGAAATGCCTTTCCAACTGGACGTTTGTACAGCACAATTCTATTTTGTGCGGGTAAGTTGTTGCGTCAGGAGGCGTTGTGGATTTCTCAATCATGGTTTACGCAGTTATTGCGTTGGTGGGTGTGGCAATTGGCTGGCTGTTTGCCAGTTATCAACATGCGCAGCAAAAAGCCGAGCAATTAGCTGA  >  NC_000913/4017154‑4017442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑