Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,109,803	G→A	D324N (GAT→AAT)	sbp →	sulfate transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,109,803	0	G	A	100.0%	133.0 / NA	39	D324N (GAT→AAT)	sbp	sulfate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/18);  total (21/18)

CGAAAAACTACTACCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGTCTTC  >  NC_000913/4109657‑4109948                                                                                                                                                   |                                                                                                                                                  cgAAAAACTACTACCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCaatc                                                                                                                                                >  2:231179/1‑150 (MQ=255)   aaaaaCTACTACCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCaatcaga                                                                                                                                             <  2:662564/151‑1 (MQ=255)     aaaCTACTACCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCaatcagatc                                                                                                                                           <  1:666009/151‑1 (MQ=255)             acCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAAc                                                                                                                                    <  1:580354/150‑1 (MQ=255)                gTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTg                                                                                                                                <  2:492513/151‑1 (MQ=255)                gTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTAAGTTCAAACAGATCAGCaaa                                                                                                                                     >  1:262013/1‑146 (MQ=255)                 tCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCt                                                                                                                                 >  2:629358/1‑149 (MQ=255)                  ccGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGAt                                                                                                                              <  2:341575/151‑1 (MQ=255)                           gCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAgg                                                                                                                     >  1:543058/1‑151 (MQ=255)                               aGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATaa                                                                                                                 <  2:2009/151‑1 (MQ=255)                                  tGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAAAGCTGATTTTCCCAGGATAAtt                                                                                                               >  1:193573/1‑150 (MQ=255)                                   ggCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTAt                                                                                                             <  1:327364/151‑1 (MQ=255)                                     cGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATc                                                                                                            >  2:19511/1‑150 (MQ=255)                                      gAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCa                                                                                                           >  2:248611/1‑150 (MQ=255)                                        aaaaGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCaaa                                                                                                         >  1:165426/1‑150 (MQ=255)                                         aaaGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAAcc                                                                                                       >  1:468155/1‑151 (MQ=255)                                                          ttCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGAc                                                                                      >  1:627631/1‑151 (MQ=255)                                                          ttCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGGTTCTCGCGAc                                                                                      >  2:479139/1‑151 (MQ=255)                                                              aaaGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCggg                                                                                  <  2:154268/151‑1 (MQ=255)                                                                  cTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCCGTGGTTTCTCGCGACCGGGtttt                                                                              >  1:268424/1‑151 (MQ=255)                                                                      aGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCa                                                                                                           <  2:382319/118‑1 (MQ=255)                                                                      aGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTAt                                                                          <  2:425502/151‑1 (MQ=255)                                                                      aGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGGTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTAt                                                                                                             >  1:382315/1‑116 (MQ=255)                                                                            tCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTc                                                                     <  1:629348/150‑1 (MQ=255)                                                                                       gaAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCg                                                          <  1:623736/150‑1 (MQ=255)                                                                                        aaGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGAACAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGtt                                                        >  2:646149/1‑151 (MQ=255)                                                                                         agagTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTAttt                                                                        >  1:618705/1‑134 (MQ=255)                                                                                         agagTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTAttt                                                                        <  2:618716/134‑1 (MQ=255)                                                                                                   ggcTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCAtc                                             <  2:543063/151‑1 (MQ=255)                                                                                                   ggcTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCAtc                                             <  2:531677/151‑1 (MQ=255)                                                                                                       ggACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCGCtt                                         <  1:530457/151‑1 (MQ=255)                                                                                                        gACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCttt                                        >  1:551380/1‑151 (MQ=255)                                                                                                         aCGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTg                                       >  2:274072/1‑151 (MQ=255)                                                                                                                    aaaaGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTTTTTGTCACGATTTTGCGGTATCCTTTCATCTCTTTGTGGTAAAgggg                            >  1:575828/1‑150 (MQ=255)                                                                                                                    aaaaGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAggg                             >  2:502699/1‑150 (MQ=255)                                                                                                                      aaGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGaaa                          <  1:299831/151‑1 (MQ=255)                                                                                                                      aaGAGCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGGGGATTTTCGCGACCGGGTTTCTTATTTGTCACGATTCTGCGTTACCCTTGCCTCTCTTTGAGGTACAGGGaa                           >  1:566965/1‑150 (MQ=255)                                                                                                                       agagCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGGTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGaaa                          >  1:532402/1‑150 (MQ=255)                                                                                                                        gagCATTTTGCTAACGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGaaaaa                        <  2:132341/151‑1 (MQ=255)                                                                                                                                    aaCGGCGGTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGaaaaaa            <  1:364488/151‑1 (MQ=255)                                                                                                                                      cggcggTACGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGc          >  1:143149/1‑151 (MQ=255)                                                                                                                                              cGTTCAATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGtcttc  <  1:486742/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGAAAAACTACTACCGTCCGCGCGACGCTGAGGTGGCGAAAAAGTACGAAAATGCGTTTCCAAAGCTGAAGTTATTCACCATTGATGAAGAGTTCGGCGGCTGGACGAAAGCGCAAAAAGAGCATTTTGCTAACGGCGGTACGTTCGATCAGATCAGCAAACGCTGATTTCCCCAGGATAATTATCAAACCCGGTGGTTTCTCGCGACCGGGTTTTTTATTTGTCACGATTTTGCGTTACCCTTGCATCTCTTTGAGGTACAGGGAAAAAAAGATGAAAAAAGCGGGTCTTC  >  NC_000913/4109657‑4109948

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑