Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,129,743	A→G	T358A (ACG→GCG)	metB →	cystathionine gamma‑synthase, PLP‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,129,743	0	A	G	100.0%	152.4 / NA	43	T358A (ACG→GCG)	metB	cystathionine gamma‑synthase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/26);  total (17/26)

TCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGG  >  NC_000913/4129607‑4129887                                                                                                                                         |                                                                                                                                                 tcgtTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCtgctgc                                                                                                                                         <  1:488039/146‑1 (MQ=255)  cgtTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTAtct                                                                                                                                   <  1:106824/151‑1 (MQ=255)            gcgggcTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTAt                                                                                                                         <  2:422768/151‑1 (MQ=255)              gggcTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTAtt                                                                                                                        <  1:394872/150‑1 (MQ=255)              gggcTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTg                                                                                                                       >  2:420413/1‑151 (MQ=255)                gcTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGa                                                                                                                      >  2:119771/1‑150 (MQ=255)                  tGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGa                                                                                                                      >  1:42192/1‑148 (MQ=255)                           ttACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGa                                                                                                             >  2:368179/1‑148 (MQ=255)                             aCGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGAt                                                                                                         <  1:540044/150‑1 (MQ=255)                              cGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGAttt                                                                                                       >  2:385412/1‑151 (MQ=255)                              cGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGAtt                                                                                                        <  2:619448/150‑1 (MQ=255)                                   gCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTg                                                                                                  <  2:563285/151‑1 (MQ=255)                                         tCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGAcc                                                                                            >  1:477115/1‑151 (MQ=255)                                              aGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGa                                                                                        <  1:546792/150‑1 (MQ=255)                                                  ggAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATg                                                                                   <  2:153025/151‑1 (MQ=255)                                                   gAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATgg                                                                                  >  2:635067/1‑151 (MQ=255)                                                       ggAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCtt                                                                               <  1:408459/150‑1 (MQ=255)                                                       ggAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTc                                                                              <  1:156028/151‑1 (MQ=255)                                                         aaaGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTATTTGCCGACCTGGAAAATGGCTTCCg                                                                            <  1:556392/151‑1 (MQ=255)                                                         aaaGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCg                                                                            <  2:56972/151‑1 (MQ=255)                                                            gTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGc                                                                         >  2:397567/1‑151 (MQ=255)                                                             tttAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCt                                                                        >  2:303053/1‑151 (MQ=255)                                                                aaTCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGc                                                                      >  2:474368/1‑150 (MQ=255)                                                                aaTCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGc                                                                      >  2:242196/1‑150 (MQ=255)                                                                aaTCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCa                                                                     <  1:589245/151‑1 (MQ=255)                                                                   ctctcACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAAc                                                                  >  1:649953/1‑151 (MQ=255)                                                                    tctcACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAAc                                                                  >  1:386073/1‑150 (MQ=255)                                                                     ctcACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCaa                                                                    <  1:79002/147‑1 (MQ=255)                                                                       cACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAgg                                                              <  2:477118/151‑1 (MQ=255)                                                                            cgcAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTa                                                          >  2:444214/1‑150 (MQ=255)                                                                                   aTGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATg                                                    >  2:597303/1‑149 (MQ=255)                                                                                      acacATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAgtgt                                               <  2:504387/151‑1 (MQ=255)                                                                                            gCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTg                                          <  1:474365/150‑1 (MQ=255)                                                                                              aGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTgcgc                                       >  1:493155/1‑151 (MQ=255)                                                                                                 cATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCgcagg                                    >  2:652579/1‑151 (MQ=255)                                                                                                     gCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGcaggcagg                                <  1:492483/151‑1 (MQ=255)                                                                                                     gCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGcaggcagg                                <  2:90918/151‑1 (MQ=255)                                                                                                                gcgTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGgtc                     <  2:566406/151‑1 (MQ=255)                                                                                                                 cgTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGgtc                     <  1:132664/150‑1 (MQ=255)                                                                                                                 cgTGCTGCCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGgtc                     <  2:217670/150‑1 (MQ=255)                                                                                                                    gctgcCGCCGGGATCTCCGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCa                 <  1:304854/151‑1 (MQ=255)                                                                                                                                    ccGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAAtt     <  2:198665/147‑1 (MQ=255)                                                                                                                                    ccGAGGCGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTtgg  <  2:434817/150‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                 TCGTTTCCTGGGCGGGCTGTCGTTGTTTACGCTGGCGGAATCATTAGGGGGAGTGGAAAGTTTAATCTCTCACGCCGCAACCATGACACATGCAGGCATGGCACCAGAAGCGCGTGCTGCCGCCGGGATCTCCGAGACGCTGCTGCGTATCTCCACCGGTATTGAAGATGGCGAAGATTTAATTGCCGACCTGGAAAATGGCTTCCGGGCTGCAAACAAGGGGTAAAAATGAGTGTGATTGCGCAGGCAGGGGCGAAAGGTCGTCAGCTGCATAAATTTGG  >  NC_000913/4129607‑4129887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑