Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,143,617	A→G	intergenic (+8/‑7)	frwC → / → frwB	putative enzyme IIC component of PTS/putative enzyme IIB component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,143,617	0	A	G	100.0%	135.4 / NA	38	intergenic (+8/‑7)	frwC/frwB	putative enzyme IIC component of PTS/putative enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/19);  total (19/19)

GTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAA  >  NC_000913/4143473‑4143762                                                                                                                                                 |                                                                                                                                                  gTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGcc                                                                                                                                             <  2:269840/151‑1 (MQ=255) gTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGcc                                                                                                                                             <  2:638346/151‑1 (MQ=255)    atatCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCAt                                                                                                                                           <  1:150198/150‑1 (MQ=255)    atatCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATg                                                                                                                                          >  2:37765/1‑151 (MQ=255)    atatCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAAAGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCAt                                                                                                                                           >  1:398569/1‑150 (MQ=255)          cagcagTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGaa                                                                                                                                     >  1:206743/1‑150 (MQ=255)                 ggCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAattatt                                                                                                                              <  2:28330/150‑1 (MQ=255)                 ggCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAattatt                                                                                                                              <  2:485351/150‑1 (MQ=255)                     gTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCa                                                                                                                           <  1:425476/149‑1 (MQ=255)                                 gtgACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTcc                                                                                                             >  2:129203/1‑151 (MQ=255)                                   gACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCtt                                                                                                           <  2:323291/151‑1 (MQ=255)                                     cGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTc                                                                                                          >  1:452519/1‑150 (MQ=255)                                         tGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGgtg                                                                                                     >  2:464673/1‑151 (MQ=255)                                          gTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGgt                                                                                                      <  2:417257/149‑1 (MQ=255)                                          gTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGgt                                                                                                      <  1:371777/149‑1 (MQ=255)                                                  gAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCAt                                                                                             <  1:639309/150‑1 (MQ=255)                                                  gAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCAt                                                                                             <  1:102192/150‑1 (MQ=255)                                                          tGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATg                                                                                    >  1:474475/1‑151 (MQ=255)                                                               aGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGgcagc                                                                               <  2:594678/151‑1 (MQ=255)                                                                gTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGgcagca                                                                              <  1:658835/151‑1 (MQ=255)                                                                           aaaaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTgg                                                                    >  1:59805/1‑150 (MQ=255)                                                                             aaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGaaa                                                                 <  2:645646/151‑1 (MQ=255)                                                                             aaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGaaa                                                                 <  1:134390/151‑1 (MQ=255)                                                                             aaaTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGaa                                                                  >  1:509420/1‑150 (MQ=255)                                                                               aTGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGt                                                               >  1:270808/1‑151 (MQ=255)                                                                                       tCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGGGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCgcga                                                        >  1:536268/1‑150 (MQ=255)                                                                                          aCTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCgcgaaagc                                                    <  2:410740/151‑1 (MQ=255)                                                                                                 aaaaaGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGc                                             >  1:51089/1‑151 (MQ=255)                                                                                                   aaaGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTg                                           >  2:480884/1‑151 (MQ=255)                                                                                                            gacCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGaa                                  <  2:51088/151‑1 (MQ=255)                                                                                                             acCTGGATTTGGATTTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGaa                                  >  1:232895/1‑150 (MQ=255)                                                                                                                tggatttggattTTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGt                               >  2:285087/1‑150 (MQ=255)                                                                                                                     ttggatttTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAAc                         >  2:212712/1‑151 (MQ=255)                                                                                                                     ttggatttTGAAATTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAAc                         >  1:336046/1‑151 (MQ=255)                                                                                                                               aaaTTAATTAATTGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCa               <  2:231396/151‑1 (MQ=255)                                                                                                                                       taattGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCt       >  1:304218/1‑151 (MQ=255)                                                                                                                                           tGAGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGa    <  2:270808/150‑1 (MQ=255)                                                                                                                                             aGGAGCGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGaaa  >  2:381017/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                                  GTTATATCGCAGCAGTGGCTGTCGGAGCAGTGGTGACGGCTGTTTGTGTGAACGTGCTGAAAAGTCTGGCGCGTAAAAATGGGTCTTCGACTGATGAAAAAGAAGACGACCTGGATTTGGATTTTGAAATTAATTAATTGAGGAACGAGCCATGACGAAAATTATTGCAGTAACCGCATGTCCTTCAGGTGTTGCCCATACCTATATGGCAGCAGAGGCGCTGGAAAGTGCCGCGAAAGCGAAAGGCTGGGAAGTGAAAGTAGAAACCCAGGGGTCAATTGGTCTGGAAA  >  NC_000913/4143473‑4143762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑