Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,307,043	C→T	A253T (GCC→ACC)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,307,043	0	C	T	97.5%	144.2 / ‑5.3	40	A253T (GCC→ACC)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (20/19);  minor base G (1/0);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

CAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGT  >  NC_000913/4306897‑4307189                                                                                                                                                   |                                                                                                                                                   cAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTggg                                                                                                                                                 >  1:636539/1‑150 (MQ=255)    aaaaCGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCat                                                                                                                                              >  1:208546/1‑150 (MQ=255)            gATGGGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCAc                                                                                                                                     <  1:190637/151‑1 (MQ=255)                 gcCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCAc                                                                                                                                >  2:227007/1‑151 (MQ=255)                     atatTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGcc                                                                                                                            >  1:308951/1‑151 (MQ=255)                                    gAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCAt                                                                                                              <  2:202557/150‑1 (MQ=255)                                    gAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATc                                                                                                             <  2:141395/151‑1 (MQ=255)                                      gcgcCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCgg                                                                                                           <  1:169645/151‑1 (MQ=255)                                                         aTGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCt                                                                                        >  1:80995/1‑151 (MQ=255)                                                             ggcggcAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCtggtg                                                                                    >  1:231444/1‑151 (MQ=255)                                                                      aaaGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGcc                                                                           <  2:610175/151‑1 (MQ=255)                                                                      aaaGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGcc                                                                           <  1:311312/151‑1 (MQ=255)                                                                        aGCGGACGACCTGATGGGGCCGAGGACGGAGCAGGGACTTTTGGGTCCAGGGAACGAGAGTCGCGCGGGGGAAGGGGGGCACATTCATGAAGCCACCGCCCAGGATCACCGCATCGGGAACGAACAGATTCATGCTGGGGGCAATGAcc                                                                           >  1:623552/1‑149 (MQ=255)                                                                          cgcACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTg                                                                       <  2:455041/151‑1 (MQ=255)                                                                             aCGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCcg                                                                    <  2:80995/151‑1 (MQ=255)                                                                               gACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCcgcg                                                                  >  2:598297/1‑151 (MQ=255)                                                                                        cGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGc                                                         >  2:343138/1‑151 (MQ=255)                                                                                        cGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGc                                                         >  2:110681/1‑151 (MQ=255)                                                                                             gTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGAct                                                    >  1:418206/1‑151 (MQ=255)                                                                                                gACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTg                                                 <  1:470532/151‑1 (MQ=255)                                                                                                    gcgcAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACg                                             <  2:418211/151‑1 (MQ=255)                                                                                                     cgcAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACg                                             >  2:342945/1‑150 (MQ=255)                                                                                                      gcAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGaa                                           <  1:349716/151‑1 (MQ=255)                                                                                                       cAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGaa                                           >  2:239101/1‑150 (MQ=255)                                                                                                       cAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGaa                                           >  1:581684/1‑150 (MQ=255)                                                                                                        aGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAg                                         <  2:197764/151‑1 (MQ=255)                                                                                                         ggTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAgg                                        >  1:347412/1‑151 (MQ=255)                                                                                                                  gggTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTc                               >  1:426026/1‑151 (MQ=255)                                                                                                                   ggTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTcc                              >  1:442832/1‑151 (MQ=255)                                                                                                                   ggTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTcc                              >  2:335547/1‑151 (MQ=255)                                                                                                                    gTCATGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCg                             <  2:325701/151‑1 (MQ=255)                                                                                                                       aTGGCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCAt                          >  1:597621/1‑151 (MQ=255)                                                                                                                         ggCAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATgg                        <  2:418047/151‑1 (MQ=255)                                                                                                                           cAACGAGAGTCTCGCGTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATgg                        >  1:567484/1‑149 (MQ=255)                                                                                                                                       cgcgTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATcgc           <  2:336033/150‑1 (MQ=255)                                                                                                                                       cgcgTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATc             <  2:597627/148‑1 (MQ=255)                                                                                                                                         cgTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCgcgc         <  1:266136/150‑1 (MQ=255)                                                                                                                                          gTGGGAAGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCa        >  2:438851/1‑150 (MQ=255)                                                                                                                                               aaGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGt  <  2:518366/151‑1 (MQ=255)                                                                                                                                               aaGGTGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGt  <  1:217377/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAGATGAGGCGGCAATAAAGCGCACGACCTGATGCGGCAGTGGACGGCGCAGGTACTTTTGGGTCATGGCAACGAGAGTCTCGCGTGGGAAGGCGGGCATATCCATCACGCCACCGCCCAGGATCACCGCATCGGGATCGAACAGATTAATGCTGGTGGCAATGGCCCGTGCCGCGTTTTCAAGCAGACTCTGGACGAAAGGGGCGTTTTCCGCATGGACGAAAAGATCGCGCAATGGGT  >  NC_000913/4306897‑4307189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑