Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,342,193 A→G T95A (ACG→GCG)  melA → alpha‑galactosidase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,342,1930AG97.5% 122.7 / NA 40T95A (ACG→GCG) melAalpha‑galactosidase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (19/20);  minor base T (1/0);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01

GGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAG  >  NC_000913/4342048‑4342328
                                                                                                                                                 |                                                                                                                                       
ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAGCTTGCGCgggg                                                                                                                                    >  1:202589/1‑149 (MQ=255)
ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCgggg                                                                                                                                    >  2:452532/1‑149 (MQ=255)
ggAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCgg                                                                                                                                      <  1:452527/149‑1 (MQ=255)
         gCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGACTGAttt                                                                                                                            >  1:593880/1‑150 (MQ=255)
            tatTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGTGAATGAt                                                                                                                              >  1:472966/1‑145 (MQ=255)
              ttGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAgg                                                                                                                       <  1:100332/150‑1 (MQ=255)
               tgtgGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGt                                                                                                                      <  2:598622/150‑1 (MQ=255)
                     gCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTaa                                                                                                                <  2:115111/150‑1 (MQ=255)
                       gTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCg                                                                                                             <  1:587365/151‑1 (MQ=255)
                          aGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCGGGGACTGATTTTGAGGTCTGTAAGCgg                                                                                                            >  2:570176/1‑149 (MQ=255)
                               aTGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTc                                                                                                     <  1:607548/151‑1 (MQ=255)
                                   aTTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTgg                                                                                                  <  1:63189/150‑1 (MQ=255)
                                         cagGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGACTGATTTCGAGGTATGTAAGGGGGAAGGGCTGGAACaaa                                                                                            >  2:645328/1‑150 (MQ=255)
                                                 aGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTTCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCa                                                                                         >  1:643092/1‑145 (MQ=255)
                                                    ggCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATa                                                                                <  1:324632/151‑1 (MQ=255)
                                                    ggCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGGCTGTAAGCGGCATGGGCTGGGACAAAACCTTGcc                                                                                    >  2:24495/1‑147 (MQ=255)
                                                     gCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGGGACTGATTTCGAGGGCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGAt                                                                                 >  2:420044/1‑149 (MQ=255)
                                                            cACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTggg                                                                         <  1:501568/150‑1 (MQ=255)
                                                                 gCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCgg                                                                    <  1:289694/150‑1 (MQ=255)
                                                                 gCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCgg                                                                    <  2:204845/150‑1 (MQ=255)
                                                                            cAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGTGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAGCCATTGCCGATACGTTGGGGCCGGGCGGtattat                                                          <  1:303796/149‑1 (MQ=255)
                                                                             agaaagaaGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATgcg                                                       <  1:577518/151‑1 (MQ=255)
                                                                             agaaagaaGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCCTGCGCGGTGACTGATTTCGGGGTCTGTAAGCGGCATGGGCTGGGACAAAACATTGCCGATACGTTGGGGCCGGGGGGgattat                                                          >  2:278308/1‑148 (MQ=255)
                                                                                aagaagCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAACCTTGCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAAACATTGCCGATACGTTGGGGCCGGGCGGTATTATgcgcg                                                     >  2:559797/1‑150 (MQ=255)
                                                                                  gaagCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATTAAACCTGTGCGGGGGCTGGTTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCAATGCCGGTAAGTTGGGGCCGGGCGGGATTATgcgcgc                                                    >  1:263942/1‑149 (MQ=255)
                                                                                     gCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTAc                                               <  2:384470/151‑1 (MQ=255)
                                                                                      ccTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACCTGCGCGGGGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGGCGGGCGGTATTATGCGCGCGCTAc                                               >  2:86623/1‑150 (MQ=255)
                                                                                       cTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGGCTGGTTTTGAGGTCTGTAAGCGGCATGGTCTGGAAAAAACCATTGCCGATAAGTTGGGGCCGGGGGGGATTATGGGCGCGCTAc                                               >  1:571824/1‑149 (MQ=255)
                                                                                          agagGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTAc                                           <  1:492082/150‑1 (MQ=255)
                                                                                                 gCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTTTGAACCTTTCTCGGTGACCGGTTTCTCGGTCTGTAAGGGGCATGGTCTGGAACAAACCACTTCCCATACGTTGGGGCCGGGGGGGATTATGCCGGCGCTACGCAcc                                          >  1:56540/1‑144 (MQ=255)
                                                                                                       tttGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCtg                              <  2:530705/150‑1 (MQ=255)
                                                                                                               ggtggCATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCa                                  <  2:279845/138‑1 (MQ=255)
                                                                                                               ggtggCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGACTGATTTTGAGGTCTGTAAGCGGCATGGTCTGGAACAAAACATTGCCGATACGGTGGGGGCGGGGGGGATTATGCGCGCGCTACGTACCATTTCGc                                   >  1:279846/1‑137 (MQ=255)
                                                                                                                gtggCATTTCAGATTGGCGGTTATGAAACTTGCGCGGGGAATGATTTTGAGGTCTGTAAGCGGCAAGGTCTGGAAAAAACCATTGCCGATAAGTTGGGGCCGGGCGGGATTATGCGCCCGCTACGTACCATTCCCCATCTGTGGCAAAttt                    >  2:148905/1‑151 (MQ=255)
                                                                                                                    cATTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGa                <  2:76722/151‑1 (MQ=255)
                                                                                                                     aTTTCAGATTGGCGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAg               <  2:339855/151‑1 (MQ=255)
                                                                                                                                 cGGTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa    >  2:340496/1‑150 (MQ=255)
                                                                                                                                  ggTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa    >  2:54336/1‑149 (MQ=255)
                                                                                                                                  ggTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGa    >  2:172092/1‑149 (MQ=255)
                                                                                                                                  ggTTATGAACCTTGCGCGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAg  <  1:232514/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                       
GGAAGAGTCGCATATTGTGGTGCGTAAGCTGATGGATTCAGCAGGGGCCAGCGGCAAAATCACCTGCCACACCCAACAGAAAGAAGCCTTAGAGGATGCCGATTTTGTCGTGGTGGCATTTCAGATTGGCGGTTATGAACCTTGCACGGTGACTGATTTCGAGGTCTGTAAGCGGCATGGTCTGGAACAAACCATTGCCGATACGTTGGGGCCGGGCGGTATTATGCGCGCGCTACGTACCATTCCGCATCTGTGGCAAATTTGCGAGGACATGACGGAAG  >  NC_000913/4342048‑4342328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑