Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,356,166	C→T	A82A (GCG→GCA)	dtpC ←	dipeptide and tripeptide permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,356,166	0	C	T	100.0%	133.3 / NA	36	A82A (GCG→GCA)	dtpC	dipeptide and tripeptide permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/17);  total (19/17)

CGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGCGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGG  >  NC_000913/4356018‑4356304                                                                                                                                                     |                                                                                                                                           cGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAgtgc                                                                                                                                          >  2:116434/1‑151 (MQ=255)             agagCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCa                                                                                                                               <  2:488000/150‑1 (MQ=255)             agagCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCa                                                                                                                               <  1:605103/150‑1 (MQ=255)              gagCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACt                                                                                                                             <  1:192851/151‑1 (MQ=255)                    gCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTg                                                                                                                       >  2:36083/1‑151 (MQ=255)                            aaCAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGcc                                                                                                               >  1:527801/1‑151 (MQ=255)                             aCAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCGGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCg                                                                                                              <  2:89844/151‑1 (MQ=255)                                gCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGc                                                                                                           >  2:306402/1‑151 (MQ=255)                                  tGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGc                                                                                                           <  1:659092/149‑1 (MQ=255)                                     tGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCg                                                                                                      >  2:213872/1‑151 (MQ=255)                                        ttgatttgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTc                                                                                                   >  2:420653/1‑151 (MQ=255)                                         tgatttgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTc                                                                                                   <  2:349589/150‑1 (MQ=255)                                           atttgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGc                                                                                                >  2:77629/1‑151 (MQ=255)                                              tgaATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGc                                                                                                >  1:468560/1‑148 (MQ=255)                                                     aCCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAgccg                                                                                      <  1:197667/151‑1 (MQ=255)                                                        gTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGccgccg                                                                                   <  1:36084/151‑1 (MQ=255)                                                                     atGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTaa                                                                      <  2:215749/151‑1 (MQ=255)                                                                       gATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACg                                                                    >  2:570285/1‑151 (MQ=255)                                                                       gATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACg                                                                    >  2:195908/1‑151 (MQ=255)                                                                            ccAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAAc                                                               <  2:373536/151‑1 (MQ=255)                                                                                 gcCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCagag                                                          <  1:314553/151‑1 (MQ=255)                                                                                   cAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAgaga                                                         >  1:448432/1‑150 (MQ=255)                                                                                   cAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAgaga                                                         >  1:63326/1‑150 (MQ=255)                                                                                      aTACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGc                                                      >  2:618046/1‑150 (MQ=255)                                                                                          aGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATg                                                 >  2:177436/1‑151 (MQ=255)                                                                                           ggCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATgc                                                >  2:405258/1‑151 (MQ=255)                                                                                               aaaGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTg                                            <  2:643204/151‑1 (MQ=255)                                                                                                   gTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACa                                        <  2:179917/151‑1 (MQ=255)                                                                                                    ttGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACa                                        <  1:213871/150‑1 (MQ=255)                                                                                                          ttGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGAt                                 >  2:55765/1‑151 (MQ=255)                                                                                                              aTCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGc                              <  1:147150/150‑1 (MQ=255)                                                                                                              aTCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGc                              <  1:258789/150‑1 (MQ=255)                                                                                                                     cccAGCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGtt                        <  1:32669/149‑1 (MQ=255)                                                                                                                         gCACCACATGGCCAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTatcatc                  >  1:62542/1‑151 (MQ=255)                                                                                                                                     cAAGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGTGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTg      >  2:575266/1‑151 (MQ=255)                                                                                                                                       aGGGTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGa     >  1:536748/1‑150 (MQ=255)                                                                                                                                         ggTCATTAACAGTGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATgg  >  2:26304/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                           CGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGATTTGAATAAACCGTAGCCACAAATAATGATTGCCAGCGCCAGATACAGGCTAAAGGTTGAATTTGTATCAATACCCAGCACCACATGGCCAAGGGTCATTAACAGCGCGCCGGCAATCACTGCAGTGCGGTTGCCGAGCAGGCGGTCGGCAAGCCAGCCGCCGAGAATAGGGGTAACGTAAACCAGAGAAGCATATGCGCTGAACAGGCTGATGGCATGGTTATCATCAAAACCAAGCTGATGG  >  NC_000913/4356018‑4356304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑