| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,357,602 | A→G | F339S (TTC→TCC) | cadA ← | lysine decarboxylase, acid‑inducible |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,357,602 | 0 | A | G | 97.8% | 169.5 / NA | 45 | F339S (TTC→TCC) | cadA | lysine decarboxylase, acid‑inducible |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/24); minor base T (0/1); total (20/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTG > NC_000913/4357454‑4357743 | ggTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTgaggag < 2:110496/151‑1 (MQ=255) tcGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTgg < 2:76471/148‑1 (MQ=255) gTCACCTTTAACGTGGATCATGGACGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCAcc > 1:661347/1‑151 (MQ=255) ttAACGTGGATCATGGAAGCCTGAGAGCACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGAACCCACGCgg > 1:535742/1‑151 (MQ=255) cGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTc < 1:256552/151‑1 (MQ=255) ggATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCaa > 2:373375/1‑150 (MQ=255) aTCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGTCTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGAGAGGAGATGGTGTAAGGCACCCACGCGGCGCCAAAGt > 1:610024/1‑151 (MQ=255) tGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGtgga > 1:109120/1‑150 (MQ=255) gCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGAtt > 2:601436/1‑151 (MQ=255) gaACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATcc > 1:426132/1‑151 (MQ=255) agcagTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCt < 2:387794/151‑1 (MQ=255) gcagTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCtt > 2:421907/1‑151 (MQ=255) gTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgat < 1:476586/151‑1 (MQ=255) gTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgat < 1:277097/151‑1 (MQ=255) tttGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatg > 2:68341/1‑151 (MQ=255) tttGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTtgatg > 2:391432/1‑151 (MQ=255) tgAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGGTGGATTTCACATCCAGTGTTTTCTTGATGAAGt > 2:58759/1‑151 (MQ=255) gTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCgg < 2:534565/151‑1 (MQ=255) gACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt < 2:595667/151‑1 (MQ=255) aCTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGTAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGgtgt < 1:102741/150‑1 (MQ=255) cGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTAcagca < 1:646336/150‑1 (MQ=255) tAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGAc > 1:375850/1‑151 (MQ=255) cACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACcatc > 2:24047/1‑150 (MQ=255) cccTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGt < 2:71325/150‑1 (MQ=255) tACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTg > 2:381389/1‑151 (MQ=255) acacGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGtt < 1:41162/149‑1 (MQ=255) acacGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGt < 2:624291/148‑1 (MQ=255) acGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTa < 1:269379/151‑1 (MQ=255) acGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTa < 2:425086/151‑1 (MQ=255) cGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTaa < 1:102165/151‑1 (MQ=255) cACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTAc < 2:253678/151‑1 (MQ=255) cACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTAc < 1:381385/151‑1 (MQ=255) gCTCATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCa < 1:615955/151‑1 (MQ=255) cATACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCAt < 1:8435/149‑1 (MQ=255) tACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGaa < 2:358866/97‑1 (MQ=255) tACCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGaa > 1:358862/1‑97 (MQ=255) aCCGCATTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTAc < 1:649667/150‑1 (MQ=255) aTTTACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCa < 2:313618/151‑1 (MQ=255) tttACCTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGACGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGcc > 1:479018/1‑149 (MQ=255) ccTTCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTg > 1:242144/1‑151 (MQ=255) tCGTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCg < 2:242145/150‑1 (MQ=255) gTAAATCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGttt > 2:526345/1‑151 (MQ=255) aaaTCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTgg > 2:553967/1‑151 (MQ=255) aTCGGTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGgtg < 1:494293/151‑1 (MQ=255) gTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGt > 1:62133/1‑141 (MQ=255) gTGAGGAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGt < 2:62132/141‑1 (MQ=255) | GGTTTCTTCGTTTACGTCACCTTTAACGTGGATCATGGAAGCCTGAGAGAACGCCGCCAGCAGTTTGTGAGTGGACTGGGTTTCGTAAATCACTTTCCCTTCTACACGGCCACCGCTCATACCGCATTTACCTTCGTAAATCGGTGAGAAGTTGGTGTAAGGCACCCACGCGGAGTCAAAGTGGATGGATTTCACATCCAGTGTTTTCTTGATGAAGTCGGTGTTGTACAGCAGACCATCATAGGTAGAGTTGGTAATTACAGCATGTACCGGCCAGGTTGCGTTTGGTG > NC_000913/4357454‑4357743 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |