Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,453,330	A→G	N254D (AAC→GAC)	ytfT →	putative sugar ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,453,330	0	A	G	100.0%	132.1 / NA	37	N254D (AAC→GAC)	ytfT	putative sugar ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/15);  total (22/15)

GTATCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTC  >  NC_000913/4453196‑4453475                                                                                                                                       |                                                                                                                                                  gTATCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATg                                                                                                                                   <  1:451795/151‑1 (MQ=255)   aTCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATgg                                                                                                                                  <  1:352460/150‑1 (MQ=255)     cAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGgct                                                                                                                                <  2:580971/150‑1 (MQ=255)                   cAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCAt                                                                                                                 <  2:616984/151‑1 (MQ=255)                     aaaaaTGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCAtt                                                                                                                >  1:108600/1‑150 (MQ=255)                     aaaaaTGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCAtt                                                                                                                >  1:438048/1‑150 (MQ=255)                      aaaaTGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATtct                                                                                                              >  2:612680/1‑151 (MQ=255)                               gggTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCggtggt                                                                                                     >  1:317060/1‑151 (MQ=255)                                   aaaCACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGAtt                                                                                                 >  1:61446/1‑151 (MQ=255)                                     acacGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTgg                                                                                               >  2:373045/1‑151 (MQ=255)                                      cacGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTggc                                                                                              <  1:74608/151‑1 (MQ=255)                                               catcGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCg                                                                                     >  2:498851/1‑151 (MQ=255)                                               catcGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCg                                                                                     >  2:153310/1‑151 (MQ=255)                                                 tcGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGc                                                                                    <  1:545680/150‑1 (MQ=255)                                                    tCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGAt                                                                                >  2:275443/1‑151 (MQ=255)                                                     cATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATg                                                                               <  1:6251/151‑1 (MQ=255)                                                               tATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGgcgc                                                                      >  1:423433/1‑150 (MQ=255)                                                                aTGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCt                                                                     <  2:391079/150‑1 (MQ=255)                                                                  gtgtTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                                                                   <  1:359975/150‑1 (MQ=255)                                                                   tgtTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCttt                                                                   >  1:106183/1‑149 (MQ=255)                                                                    gtTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAAc                                                                <  2:438053/151‑1 (MQ=255)                                                                      tGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAAcc                                                               <  2:125326/150‑1 (MQ=255)                                                                      tGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAAcc                                                               <  2:209835/150‑1 (MQ=255)                                                                       gAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTg                                                             >  1:522108/1‑151 (MQ=255)                                                                            ggCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACt                                                        >  1:458767/1‑151 (MQ=255)                                                                                      ggcgATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGCTGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGgtggtg                                              >  2:607610/1‑151 (MQ=255)                                                                                         gATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTgggg                                           >  1:299852/1‑151 (MQ=255)                                                                                                       tCGTGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGattatt                               >  2:296820/1‑149 (MQ=255)                                                                                                          tGGCGGCGGATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAggg                          >  1:430987/1‑151 (MQ=255)                                                                                                              ggcggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATg                      >  2:221469/1‑151 (MQ=255)                                                                                                              ggcggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATg                      >  2:553841/1‑151 (MQ=255)                                                                                                              ggcggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATg                      <  2:106186/151‑1 (MQ=255)                                                                                                                cggATATTCGCGGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGa                     >  2:184458/1‑150 (MQ=255)                                                                                                                        cgcgGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGaa            <  1:236450/151‑1 (MQ=255)                                                                                                                         gcgGTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAAt           >  1:596333/1‑151 (MQ=255)                                                                                                                           ggTGCCGATGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAAttt         >  1:508719/1‑151 (MQ=255)                                                                                                                                  aTGCCGACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTc  <  2:278943/151‑1 (MQ=255)                                                                                                                                       |                                                                                                                                                  GTATCAACATTCGGGCGGCAAAAAATGCCGGGGTAAACACGCGAATCATCGTCATGCTCACTTATGTGTTGAGCGGGCTGTGTGCGGCGATTGCGGGCATTATCGTGGCGGCGGATATTCGCGGTGCCGATGCCAACAACGCTGGGTTATGGCTGGAGCTGGACGCCATTCTCGCGGTGGTGATTGGCGGCGGATCGCTGATGGGCGGGCGCTTTAACCTGCTACTTTCGGTGGTGGGGGCGCTGATTATTCAGGGGATGAACACCGGAATTTTGCTTTC  >  NC_000913/4453196‑4453475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑