Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,550,206	G→A	A364V (GCC→GTC)	gntP ←	fructuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,550,206	0	G	A	100.0%	92.5 / NA	24	A364V (GCC→GTC)	gntP	fructuronate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/11);  total (13/11)

ACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTC  >  NC_000913/4550061‑4550352                                                                                                                                                  |                                                                                                                                                   aCCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTc                                                                                                                                               <  1:380332/151‑1 (MQ=255)        aacaacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCg                                                                                                                                        <  1:198039/151‑1 (MQ=255)        aacaacCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCg                                                                                                                                        >  1:539613/1‑151 (MQ=255)               aGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGac                                                                                                                                 >  1:190411/1‑151 (MQ=255)                   aGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACAccc                                                                                                                             >  1:172028/1‑151 (MQ=255)                            gTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTc                                                                                                                    <  2:441292/151‑1 (MQ=255)                             ttAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTc                                                                                                                    >  1:267384/1‑150 (MQ=255)                             ttAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCg                                                                                                                   >  1:343001/1‑151 (MQ=255)                                aTGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGcc                                                                                                                 >  2:35809/1‑150 (MQ=255)                                   tgGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGAc                                                                                                             >  1:401757/1‑151 (MQ=255)                                                      ccGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTGGCCAGACGAATTAGCACAGTGATCAg                                                                                          <  1:8193/151‑1 (MQ=255)                                                                cGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGccatgccatg                                                                                <  1:207600/151‑1 (MQ=255)                                                                 gCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGccatgccatg                                                                                <  1:95895/150‑1 (MQ=255)                                                                                             cAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCTAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACAt                                                   >  2:409250/1‑151 (MQ=255)                                                                                               aCCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATc                                                  >  1:669905/1‑150 (MQ=255)                                                                                               aCCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCa                                                 <  2:120013/151‑1 (MQ=255)                                                                                                 cAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAAc                                               <  1:256068/151‑1 (MQ=255)                                                                                                      gACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGcc                                          <  2:625341/151‑1 (MQ=255)                                                                                                                tGGATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCg                                >  2:46990/1‑151 (MQ=255)                                                                                                                 ggATCTAGGATTGCAGCACTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGc                               <  1:344269/151‑1 (MQ=255)                                                                                                                               agcaCTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCg                 >  2:208900/1‑151 (MQ=255)                                                                                                                                gcaCTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCg                 >  1:225070/1‑150 (MQ=255)                                                                                                                                  aCTGATAATCCCGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCgatg              >  2:469543/1‑151 (MQ=255)                                                                                                                                            ccGGCGACGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCt    <  1:468012/151‑1 (MQ=255)                                                                                                                                             cggcgacggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCtt   <  1:95720/151‑1 (MQ=255)                                                                                                                                              ggcgacggTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTc  <  2:172030/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ACCTTTGAACAACCAGAAAGATGCATCGTTAATGTGGGTGAGGGTGTTGGAACCCGCAGCCGTCGCCAGTACCAGCAGCGCCGGATTCACGCCAACCAGCTGACCAGTTGCTGGATCTAGGATTGCAGCACTGATAATCCCGGCGGCGGTCATCGCCGAAACGACACCCTGACCCGTCGCCAGACGAATTAGCACAGTGATCAGCCATGCCATGATGTAGGGCGAGATATTGCCGTGGGACATCAACATGCCGATGGTGTCGCCAATGCCGGTGTCGATGATGGTCTGCTTC  >  NC_000913/4550061‑4550352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑