Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,348	A→G	intergenic (‑388/‑141)	mokC ← / → nhaA	regulatory protein for HokC, overlaps CDS of hokC/sodium‑proton antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,348	0	A	G	100.0%	161.5 / NA	44	intergenic (‑388/‑141)	mokC/nhaA	regulatory protein for HokC, overlaps CDS of hokC/sodium‑proton antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/23);  total (21/23)

TATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAACGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAAT  >  NC_000913/17203‑17483                                                                                                                                                  |                                                                                                                                        tattTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCAt                                                                                                                                    >  1:157008/1‑151 (MQ=255)      ttGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCAc                                                                                                                               >  2:506369/1‑151 (MQ=255)                 gCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGgcg                                                                                                                    >  2:140319/1‑151 (MQ=255)                      aCAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAAt                                                                                                               <  1:288190/151‑1 (MQ=255)                      aCAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAAt                                                                                                               <  1:356269/151‑1 (MQ=255)                               gCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAAt                                                                                                       >  1:109717/1‑150 (MQ=255)                               gCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATa                                                                                                      <  1:251807/151‑1 (MQ=255)                               gCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATa                                                                                                      <  1:436785/151‑1 (MQ=255)                                 cGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGc                                                                                                    >  2:223456/1‑151 (MQ=255)                                    cTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCg                                                                                                 >  2:595938/1‑151 (MQ=255)                                     tGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGt                                                                                                >  1:443575/1‑151 (MQ=255)                                             aattaattATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACg                                                                                        >  2:193741/1‑151 (MQ=255)                                              attaattaTTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACg                                                                                        >  2:89931/1‑150 (MQ=255)                                               ttaattaTTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGa                                                                                       >  2:277705/1‑150 (MQ=255)                                                 aattaTTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAat                                                                                     <  1:666026/150‑1 (MQ=255)                                                  attattACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAatta                                                                                   <  2:134970/151‑1 (MQ=255)                                                        aCTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAAttct                                                                                                            >  2:283963/1‑120 (MQ=255)                                                        aCTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAAttct                                                                                                            <  1:283964/120‑1 (MQ=255)                                                        aCTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCt                                                                              >  1:79311/1‑150 (MQ=255)                                                         cTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTa                                                                             <  2:558323/150‑1 (MQ=255)                                                               cTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACtat                                                                       >  1:390464/1‑150 (MQ=255)                                                                 aattaatCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACtata                                                                      <  2:480843/149‑1 (MQ=255)                                                                 aattaatCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACtata                                                                      <  2:465042/149‑1 (MQ=255)                                                                 aattaatCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACtata                                                                      >  1:480840/1‑149 (MQ=255)                                                                      atCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGAt                                                               <  2:448289/151‑1 (MQ=255)                                                                           tCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTa                                                           >  2:590434/1‑150 (MQ=255)                                                                                   ccTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGAtt                                                  >  1:275857/1‑151 (MQ=255)                                                                                     tCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCg                                                >  2:593251/1‑151 (MQ=255)                                                                                      cACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCACAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCg                                                <  2:350261/150‑1 (MQ=255)                                                                                            aaGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCgggg                                         <  2:10282/151‑1 (MQ=255)                                                                                             aGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTTCGCTATAATCTGATTTTAACGATGATTCGTGCg                                            <  2:551053/147‑1 (MQ=255)                                                                                                          tAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTaaaa                            >  1:130960/1‑150 (MQ=255)                                                                                                           aGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTTCACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTaaa                             <  1:506366/148‑1 (MQ=255)                                                                                                           aGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATGTTAACGATGATTCGTGCGGGGTAAAATAGTaaaa                            <  1:147483/149‑1 (MQ=255)                                                                                                             gCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACg                         >  1:375249/1‑150 (MQ=255)                                                                                                                      cACTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCa                <  2:633379/150‑1 (MQ=255)                                                                                                                       aCTCGTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCAcc              <  1:93382/151‑1 (MQ=255)                                                                                                                           gTGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGaa          <  2:130964/151‑1 (MQ=255)                                                                                                                            tGAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGaa          >  2:297514/1‑150 (MQ=255)                                                                                                                             gAGCGCTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAag        <  1:601808/151‑1 (MQ=255)                                                                                                                               gcgcTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAagag      <  1:118103/151‑1 (MQ=255)                                                                                                                                cgcTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAgaga     <  2:305897/151‑1 (MQ=255)                                                                                                                                cgcTTACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAgaga     <  2:208590/151‑1 (MQ=255)                                                                                                                                    tACAGCCGTCAAAAGCGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAAt  >  2:418707/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                        TATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGTCAAAAACGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAAT  >  NC_000913/17203‑17483

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑