Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	481,469	A→G	S979P (TCG→CCG)	acrB ←	multidrug efflux system protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	481,469	0	A	G	100.0%	118.4 / NA	33	S979P (TCG→CCG)	acrB	multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

GAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAAC  >  NC_000913/481329‑481596                                                                                                                                             |                                                                                                                                gAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAgg                                                                                                                       >  1:285940/1‑151 (MQ=255)    tACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATc                                                                                                                    >  1:383876/1‑151 (MQ=255)       cGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGa                                                                                                                 <  2:226127/151‑1 (MQ=255)                                 ttGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCa                                                                                         >  1:417310/1‑149 (MQ=255)                                   gCGGTCACCATGCCGCCGATTAGACCGGTACCTACTGCGTTCGGCGCGCCGGAACCAGCACCAGAACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGACATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCg                                                                                     >  1:521818/1‑151 (MQ=255)                                   gCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCg                                                                                     <  2:359745/151‑1 (MQ=255)                                    cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGt                                                                                    >  2:135489/1‑151 (MQ=255)                                    cGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGt                                                                                    <  1:328283/151‑1 (MQ=255)                                       tCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGc                                                                                 >  1:387417/1‑151 (MQ=255)                                        cACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCt                                                                                >  2:70140/1‑151 (MQ=255)                                         aCCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCtt                                                                               <  1:636315/151‑1 (MQ=255)                                             tCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAAt                                                                           >  1:646890/1‑151 (MQ=255)                                               ccGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCa                                                                         <  2:493742/151‑1 (MQ=255)                                                       tACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCttt                                                                  >  1:473289/1‑150 (MQ=255)                                                           ccGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACctt                                                             <  2:307879/151‑1 (MQ=255)                                                                aCCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCttt                                                         <  1:1276/150‑1 (MQ=255)                                                                    aCTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCa                                                    <  1:135485/151‑1 (MQ=255)                                                                              gcgcgcCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTc                                             <  2:196748/148‑1 (MQ=255)                                                                              gcgcgcCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCtt                                           <  2:517504/150‑1 (MQ=255)                                                                                gcgcCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCTGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTgg                                        <  2:93384/151‑1 (MQ=255)                                                                                  gcCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCg                                      <  1:221341/151‑1 (MQ=255)                                                                                       aaCCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTc                                 <  2:183644/151‑1 (MQ=255)                                                                                       aaCCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTc                                 >  2:212220/1‑151 (MQ=255)                                                                                                 aGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAgg                       >  2:443856/1‑151 (MQ=255)                                                                                                 aGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAgg                       <  1:157230/151‑1 (MQ=255)                                                                                                    aCTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGAt                     <  1:458141/150‑1 (MQ=255)                                                                                                    aCTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATc                    <  1:383238/151‑1 (MQ=255)                                                                                                      tGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATcgc                  >  1:619441/1‑151 (MQ=255)                                                                                                                 ggCATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCg       <  2:342072/151‑1 (MQ=255)                                                                                                                   cATAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGAc     >  2:131560/1‑151 (MQ=255)                                                                                                                    aTAACGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGAc     >  2:590584/1‑150 (MQ=255)                                                                                                                      aaCGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAAc  >  1:575169/1‑151 (MQ=255)                                                                                                                      aaCGCCGAGGATAAACGCCAGCGGGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAAc  >  1:419152/1‑151 (MQ=255)                                                                                                                                             |                                                                                                                                GAATACCGGAACGAAGAAGATTGCCAGTACCGTTGCGGTCACCATCCCGCCCATTACACCGGTACCTACTGCGTTCTGCGCGCCGGAACCAGCACCAGTACTGATAACCAGCGGCATAACGCCGAGGATAAACGCCAGCGAGGTCATCAGGATCGGACGTAAACGCATCCGCACCGCATCAAGCGTCGCTTCAATCAGACCTTTACCTTCTTTATCCATCAAGTCTTTGGCGAATTCGACGATAAGGATCGCGTTCTTCGCCGACAAC  >  NC_000913/481329‑481596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑