Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	113,104	A→G	V39A (GTG→GCG)	coaE ←	dephospho‑CoA kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	113,104	0	A	G	100.0%	175.4 / NA	47	V39A (GTG→GCG)	coaE	dephospho‑CoA kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/28);  total (19/28)

CAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATT  >  NC_000913/112959‑113249                                                                                                                                                  |                                                                                                                                                  cagGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTg                                                                                                                                               >  2:343022/1‑150 (MQ=255)  agGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGAc                                                                                                                                             <  1:141605/151‑1 (MQ=255)  agGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGAc                                                                                                                                             >  2:451725/1‑151 (MQ=255)   gggCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGAcg                                                                                                                                            >  2:311715/1‑151 (MQ=255)          aaGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCaata                                                                                                                                     <  2:99823/151‑1 (MQ=255)           aGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCaata                                                                                                                                     >  2:467781/1‑150 (MQ=255)             ccAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAataata                                                                                                                                  <  1:343256/151‑1 (MQ=255)              cAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAatat                                                                                                                                 <  1:353967/151‑1 (MQ=255)               aGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATc                                                                                                                                <  2:96603/151‑1 (MQ=255)               aGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATc                                                                                                                                >  2:30709/1‑151 (MQ=255)                  ttttCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGc                                                                                                                             >  1:521264/1‑151 (MQ=255)                              ggTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGtt                                                                                                                 <  2:272634/151‑1 (MQ=255)                               gTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTa                                                                                                                <  2:580666/151‑1 (MQ=255)                                 tGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAAt                                                                                                              >  1:33977/1‑151 (MQ=255)                                  ggCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAAtt                                                                                                             <  1:451720/151‑1 (MQ=255)                                   gCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTc                                                                                                            <  1:434774/151‑1 (MQ=255)                                     gAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCg                                                                                                          >  2:109743/1‑151 (MQ=255)                                       aGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCg                                                                                                          >  1:39739/1‑149 (MQ=255)                                             gCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGc                                                                                                  >  1:290770/1‑151 (MQ=255)                                                cgcgCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCaa                                                                                                <  1:417831/150‑1 (MQ=255)                                                 gcgcAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAAc                                                                                              <  2:651643/151‑1 (MQ=255)                                                           cGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCaa                                                                                    <  1:605511/151‑1 (MQ=255)                                                            ggCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAAc                                                                                   <  2:518740/151‑1 (MQ=255)                                                                        ttCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATAGGCAACGGCACTCTTGcc                                                                       >  1:138743/1‑151 (MQ=255)                                                                              cagcagCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGc                                                                  <  2:295883/150‑1 (MQ=255)                                                                                  agcaATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATg                                                             <  1:145833/151‑1 (MQ=255)                                                                                      aTCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTc                                                         >  2:367053/1‑151 (MQ=255)                                                                                           gTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGtt                                                    <  1:30710/151‑1 (MQ=255)                                                                                            ttAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGtt                                                    >  1:35484/1‑150 (MQ=255)                                                                                                cgcCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGc                                               <  2:411301/151‑1 (MQ=255)                                                                                                         gATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACtatat                                       >  1:530828/1‑150 (MQ=255)                                                                                                          aTCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACtatata                                      >  1:349095/1‑150 (MQ=255)                                                                                                            cAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATAcc                                    <  2:329251/150‑1 (MQ=255)                                                                                                                aaTGGCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATa                               <  2:362511/151‑1 (MQ=255)                                                                                                                    gCATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAAttt                           <  2:17367/151‑1 (MQ=255)                                                                                                                     cATGTAGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTta                          <  2:35483/151‑1 (MQ=255)                                                                                                                          aGCGCAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATAtt                      <  1:109740/150‑1 (MQ=255)                                                                                                                           gcgcAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTcc                    <  1:311713/151‑1 (MQ=255)                                                                                                                           gcgcAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTcc                    <  2:293862/151‑1 (MQ=255)                                                                                                                             gcAGGTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCg                  >  1:195338/1‑151 (MQ=255)                                                                                                                                ggTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGa               <  1:472698/151‑1 (MQ=255)                                                                                                                                ggTGCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCGCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGa               <  1:220752/151‑1 (MQ=255)                                                                                                                                   gCACCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCCCATAATTTATATTCCCGGGAAt             <  1:279943/150‑1 (MQ=255)                                                                                                                                     aCCTGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtc           >  2:50114/1‑150 (MQ=255)                                                                                                                                        tGGTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtcatc        >  1:522526/1‑150 (MQ=255)                                                                                                                                         ggTTCAACCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATtcatc        <  2:320962/149‑1 (MQ=255)                                                                                                                                              aaCCGCCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGAtt  >  2:61178/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CAGGGCGTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAACCACCTGACGCGCAATAATATCGGCATCAATGACGTTAATTCCGAGATCAGCAAACGCATTGGCAACGGTACTCTTGCCACTGCCAATGCCTCCCGTTAAGGCAACTATATACCTCATAATTTATATTCCCGGGAATTCATCATGATT  >  NC_000913/112959‑113249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑