Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	588,485	G→A	R824C (CGC→TGC)	nfrA ←	bacteriophage N4 receptor, outer membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	588,485	0	G	A	100.0%	163.7 / NA	42	R824C (CGC→TGC)	nfrA	bacteriophage N4 receptor, outer membrane subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/30);  total (12/30)

TCGGGTGCCATTCGTCGCTGTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCGCAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGT  >  NC_000913/588338‑588631                                                                                                                                                    |                                                                                                                                                   tCGTGTGCCATTCTTCGCTGAATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAACAGATCTGATCGCGCAGCGGCTTCCAGcaca                                                                                                                                                  <  1:511095/150‑1 (MQ=255)     gTGCCATTCGTCGCTGTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCg                                                                                                                                             <  1:201078/151‑1 (MQ=255)       gCCATTCGTCGCTGTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGt                                                                                                                                           <  2:288438/151‑1 (MQ=255)           ttCGTCGCTGTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCg                                                                                                                                       >  2:561157/1‑151 (MQ=255)              gtcgCTGTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGAc                                                                                                                                    >  2:97848/1‑151 (MQ=255)                   tGTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCAAGCACAGACCGGTGCCGGACATCgg                                                                                                                               >  2:162357/1‑151 (MQ=255)                    gTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCgg                                                                                                                               <  1:270430/150‑1 (MQ=255)                    gTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGa                                                                                                                              <  1:386848/151‑1 (MQ=255)                     tATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGa                                                                                                                              <  1:429489/150‑1 (MQ=255)                          gccgccATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTc                                                                                                                        >  2:167485/1‑151 (MQ=255)                             gccATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCAcc                                                                                                                     <  1:212924/151‑1 (MQ=255)                                aTTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGc                                                                                                                  <  2:59249/151‑1 (MQ=255)                                      gAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCatcatc                                                                                                            >  1:634870/1‑151 (MQ=255)                                           aGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCAcccc                                                                                                       <  2:637675/151‑1 (MQ=255)                                           aGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCAcccc                                                                                                       >  1:55006/1‑151 (MQ=255)                                           aGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCAcccc                                                                                                       <  2:221552/151‑1 (MQ=255)                                                cTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGtttt                                                                                                  >  2:429804/1‑151 (MQ=255)                                                  ggCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTtc                                                                                                 <  1:481771/150‑1 (MQ=255)                                                  ggCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTtc                                                                                                 <  2:92522/150‑1 (MQ=255)                                                       gcagcaTGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGt                                                                                           <  1:241339/151‑1 (MQ=255)                                                       gcagcaTGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGt                                                                                           <  1:14284/151‑1 (MQ=255)                                                            aTGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCgg                                                                                      <  2:362887/151‑1 (MQ=255)                                                                 aTCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCaaa                                                                                  <  1:527984/150‑1 (MQ=255)                                                                   cGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGAc                                                                               <  1:465996/151‑1 (MQ=255)                                                                    ggATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGAcg                                                                              <  2:498272/151‑1 (MQ=255)                                                                      aTGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGAcgcg                                                                            <  1:498399/151‑1 (MQ=255)                                                                      aTGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGAcgcg                                                                            <  1:260005/151‑1 (MQ=255)                                                                       tGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCgg                                                                           >  2:594125/1‑151 (MQ=255)                                                                           ccATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCtat                                                                       <  2:561955/151‑1 (MQ=255)                                                                                    ccGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTg                                                                 >  2:275406/1‑148 (MQ=255)                                                                                      gTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGc                                                            <  1:275406/151‑1 (MQ=255)                                                                                        tCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCa                                                           <  2:104190/150‑1 (MQ=255)                                                                                           gCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTc                                                       <  2:319935/151‑1 (MQ=255)                                                                                            cGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCg                                                      <  2:301723/151‑1 (MQ=255)                                                                                                       gTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCcagcag                                           <  2:25051/151‑1 (MQ=255)                                                                                                       gTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCcagca                                            <  1:240473/150‑1 (MQ=255)                                                                                                       gTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCcagc                                             <  1:418141/149‑1 (MQ=255)                                                                                                                    gAAAAAGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCg                              >  1:393648/1‑151 (MQ=255)                                                                                                                         aGATCTGATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCg                         <  2:37361/151‑1 (MQ=255)                                                                                                                               gATCGCGCAGCGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTc                   <  1:262048/151‑1 (MQ=255)                                                                                                                                         cGGCTTCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGt         <  2:615326/151‑1 (MQ=255)                                                                                                                                             ttCCAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTc     >  2:307544/1‑151 (MQ=255)                                                                                                                                                cAGCACAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGt  >  1:154799/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TCGGGTGCCATTCGTCGCTGTATTTGCCGCCATTAAAGAATGAGGCGCTGGCGCGCAGCATGGTATCGGATGCGCCATTTTGGCCGTTCAGCGGCAACTGCTGTTCGACGGCGATGAAAAAGATCTGATCGCGCAGCGGCTTCCAGCGCAGACCGGTGCCGGACATCGGATTTTTCACCGGCATCATCACCCCGTTTTCTCCGGTATCGGCAAAGACGCGGCTATAAACTGAGAGCAGGTCGCCTTCCAGCAGCATATTGCGTCCGATGCGGTACTCGGCTTCCAGTTGTCCGT  >  NC_000913/588338‑588631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑