| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 650,431 | C→T | V20V (GTG→GTA) | citE ← | citrate lyase, citryl‑ACP lyase (beta) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,431 | 0 | C | T | 96.9% | 106.5 / ‑5.5 | 32 | V20V (GTG→GTA) | citE | citrate lyase, citryl‑ACP lyase (beta) subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (18/13); total (18/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
GATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCA > NC_000913/650286‑650572 | gATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTacaaa < 1:443737/151‑1 (MQ=255) cAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAaacaac < 2:274921/151‑1 (MQ=255) gTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTgcg > 2:324691/1‑151 (MQ=255) ttGCAGCGCGTGGTAAACCATGCGGCGGGCGGGGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTgcgg > 2:98610/1‑151 (MQ=255) ccAGCGCGTGGTAAACCATGCGGCGGGCGGGGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATAAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTgcggc‑g > 1:650526/2‑151 (MQ=255) aGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑ggg > 1:556653/1‑151 (MQ=255) cgcgTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGGCAAACATCAGGGCAACAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCCCCAGGTACAAACAACATGCTGCGGC‑ggg > 1:479127/1‑149 (MQ=255) gcgTGGTAAACCATGCGTCGGTCTGTGTCTTTTTCACGCAATGATCCTGAGTCTCCGAGGTCAAACATCCGCGCATCCGTCGGGTCGTTGAAGGAGTTGCTTCCCTTCTCCGCCTTGGCTCCACGCATTAAAAACATGCTGGGGG‑GGGTgg < 1:285776/151‑2 (MQ=255) aTGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACgtt < 1:98608/151‑1 (MQ=255) gcggcgGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACgttgt < 2:378994/151‑1 (MQ=255) ggcggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGc < 2:416535/151‑1 (MQ=255) cggTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGa < 2:398332/151‑1 (MQ=255) tCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGTTTTTTGTTGTTGCAGCGAAGCgg > 1:66974/1‑151 (MQ=255) tttttCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGGTTTACGTTGTTggagcggagccg > 1:60220/1‑147 (MQ=255) tCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGGGGGGTTTTCGTTGTTGCAGCGAAGAGG‑gaa > 2:502713/1‑148 (MQ=255) aCGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGGGTTTTTCGTTGTTTCAGCGAAGCGG‑GAATCAtt > 2:395742/1‑151 (MQ=255) aCGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCAtt < 1:354114/151‑1 (MQ=255) aaTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGGTTTACGTTGTTGGAGAGAAGCGG‑AAATCAtttg > 2:386759/1‑147 (MQ=255) aaTGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAAACATTGGc > 2:294184/1‑150 (MQ=255) cTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGGGGTTTTTGTTGTTGCAGGGAAGAGG‑GAAACATTTGCAAtcc > 1:67779/1‑151 (MQ=255) ggAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGAGGTTTTCGTTGTTGCAGCGGAGCGG‑AAATAATTTGCAATCCTccc > 2:461242/1‑151 (MQ=255) tCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGAGTTTTTCGTTGTTTCAGCGAAACGG‑GAATCATTGGCAATCCCCCCCTgg > 2:183219/1‑151 (MQ=255) tCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGc < 2:614648/149‑1 (MQ=255) aGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGCGGGGGGGGGGTTTTAGGTT‑TTGGAGGGAAGAGGAAAATCTTTGGAATTcccccctcg > 1:257779/1‑142 (MQ=255) ggTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGcc < 1:324689/151‑1 (MQ=255) aaCATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGCGGGGTTTTTGTTGTTTCAGCGAAGCGG‑GAAACATTTGCAATCCTCCCCCGGCAGAGCCggg > 1:614844/1‑149 (MQ=255) cATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGGGGGGGGTTTTCGTTGTTGCCGCGAAGCGG‑AAATCATTTGCCATCCTCCCATGGCAGAGCCggg > 1:485157/1‑147 (MQ=255) cATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGgcg < 2:375944/151‑1 (MQ=255) cAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGt < 1:396773/151‑1 (MQ=255) aCCATCGCGGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGGGGGGTTTTCGTTGTTGTAGTGGAGCGG‑GAAACATTGGGAATCCTCCCCTGGCAGAGACGGGGGAACCCCGGGGCGTGGCAACCGGGGTTccccgcgtg > 1:515614/1‑140 (MQ=255) cgcgGCATTGGCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTaaaa < 2:521447/151‑1 (MQ=255) ggCACCAGGTACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCa < 1:139959/151‑1 (MQ=255) | GATACAGCGGATGTTGCAGCGCGTGGTAAACCATGCGGCGGGCGGTGTCTTTTTCACGCAATGCTACGGAGTCTTCGAGGTCAAACATCAGGGCATCAGCCGGGTAGATGAAGGAGTTGCTGACCATCGCGGCATTGGCACCAGGCACAAACAACATGCTGCGGC‑GGGTGCGAGTTTTACGTTGTTGCAGCGAAGCGG‑AAATCATTGGCAATCCTCCCATGGCAGAGCCGGGATACCGCTGGCGCGTGCCAGCAGGGCTTCCAGTCGTGCACGTAAAATGCAGTCCA > NC_000913/650286‑650572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |