Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	717,098	A→G	F390L (TTT→CTT)	potE ←	putrescine/proton symporter: putrescine/ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	717,098	0	A	G	100.0%	100.7 / NA	28	F390L (TTT→CTT)	potE	putrescine/proton symporter: putrescine/ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/13);  total (15/13)

CGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAG  >  NC_000913/716951‑717233                                                                                                                                                    |                                                                                                                                        cGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGtt                                                                                                                                      <  2:651148/151‑1 (MQ=255)    gtTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGc                                                                                                                                   <  2:206043/151‑1 (MQ=255)    gtTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGc                                                                                                                                   <  2:104716/151‑1 (MQ=255)      ttATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCa                                                                                                                                  >  1:413069/1‑150 (MQ=255)            ttCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCg                                                                                                                           >  2:137999/1‑151 (MQ=255)             tCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCg                                                                                                                           <  1:43034/150‑1 (MQ=255)               aGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCtt                                                                                                                        >  2:48955/1‑151 (MQ=255)                    aaaGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTg                                                                                                                     >  1:196853/1‑149 (MQ=255)                           ggtgATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGca                                                                                                            <  1:113043/151‑1 (MQ=255)                              gATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGcaca                                                                                                          <  1:323056/150‑1 (MQ=255)                                      aCCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGTGCACATTAGCGAcc                                                                                                 <  1:515409/151‑1 (MQ=255)                                       ccATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCt                                                                                                >  2:276687/1‑151 (MQ=255)                                                     accaaGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACt                                                                                  >  2:263088/1‑151 (MQ=255)                                                             aggTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATgccgc                                                                          <  2:518958/151‑1 (MQ=255)                                                                  aCGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTg                                                                     <  1:137646/151‑1 (MQ=255)                                                                   cGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGa                                                                    <  1:601069/151‑1 (MQ=255)                                                                                cAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACgg                                                        >  2:33612/1‑150 (MQ=255)                                                                                 aGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGaa                                                      <  1:665743/151‑1 (MQ=255)                                                                                   cATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATg                                                    >  2:347846/1‑151 (MQ=255)                                                                                           cttcCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGt                                            >  2:76011/1‑151 (MQ=255)                                                                                               cccGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTcacc                                        >  2:382797/1‑151 (MQ=255)                                                                                                      gAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCa                                 <  2:56092/151‑1 (MQ=255)                                                                                                              cgcATAAAAGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAAcc                         >  1:616076/1‑151 (MQ=255)                                                                                                                     aaGCTATACATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGt                  >  2:475611/1‑151 (MQ=255)                                                                                                                           taCATCGCGCCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAact            >  2:661450/1‑151 (MQ=255)                                                                                                                                   gcCAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGttt    >  1:101506/1‑151 (MQ=255)                                                                                                                                    ccAACAAAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGttt    >  2:352549/1‑150 (MQ=255)                                                                                                                                     caacaaAAGCAACAAGGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAg  <  2:482971/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                        CGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATGCCGCCATTGACAGAATATACGGAATGATATTGGTCACCACGGCCAGGTTAACCAGCACGTTGAACTGACTGTTTAG  >  NC_000913/716951‑717233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑