Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	741,429	A→G	S376S (TCT→TCC)	dtpD ←	dipeptide and tripeptide permease D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	741,429	0	A	G	100.0%	89.1 / NA	25	S376S (TCT→TCC)	dtpD	dipeptide and tripeptide permease D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/11);  total (14/11)

GCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCT  >  NC_000913/741288‑741558                                                                                                                                              |                                                                                                                                  gcCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTg                                                                                                                           >  2:612597/1‑150 (MQ=255)   cAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGAcc                                                                                                                        >  1:120549/1‑151 (MQ=255)         gcagcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACAt                                                                                                                  <  1:342418/151‑1 (MQ=255)          cagcaTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACAtt                                                                                                                 <  1:12374/151‑1 (MQ=255)             caTGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGc                                                                                                               <  1:511949/150‑1 (MQ=255)                       ccGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCggg                                                                                                    <  1:34998/151‑1 (MQ=255)                        cGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGAACCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCCGCgg                                                                                                     >  1:400507/1‑149 (MQ=255)                           ttAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCt                                                                                                >  2:239370/1‑151 (MQ=255)                             aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTa                                                                                              <  2:643345/151‑1 (MQ=255)                             aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTa                                                                                              <  1:549822/151‑1 (MQ=255)                              aTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTa                                                                                              >  2:83778/1‑150 (MQ=255)                                          cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGc                                                                                  <  2:139041/150‑1 (MQ=255)                                                    ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGgcg                                                                       >  2:173345/1‑151 (MQ=255)                                                       aaTGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCtc                                                                    >  1:645516/1‑151 (MQ=255)                                                                        aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGgccaaggccaag                                                   <  2:248346/151‑1 (MQ=255)                                                                          aTGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGgccaaggccaag                                                   >  2:159668/1‑149 (MQ=255)                                                                                aCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAAtt                                           >  2:256543/1‑151 (MQ=255)                                                                                        gATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAg                                   <  1:438424/151‑1 (MQ=255)                                                                                            aaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgc                               <  1:304937/151‑1 (MQ=255)                                                                                               aGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATgcgc                             >  2:640293/1‑150 (MQ=255)                                                                                                     gCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTa                       <  2:452778/150‑1 (MQ=255)                                                                                                        aaGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGAt                   >  1:453886/1‑151 (MQ=255)                                                                                                                      cAGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActc     >  1:400611/1‑151 (MQ=255)                                                                                                                       aGGCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActct    >  1:260282/1‑151 (MQ=255)                                                                                                                         gCCTAATACCATCAGTGGCAGGGAAGAGTGACCATACATTGCGGACAAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACActctct  >  1:12075/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                  GCCAGAAAGCAGCATGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAGCGGGCGCTTAAGGTCAGAATGCAGAATCCGGCGCTCATCAGGCCAAGGCCAAGAGCAAATTTCCCCCAGATGCGCACGGTACGATTACCCGCGACACTCTCT  >  NC_000913/741288‑741558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑