Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	835,945	A→G	Y233C (TAT→TGT)	ybiB →	putative family 3 glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	835,945	0	A	G	100.0%	99.6 / NA	32	Y233C (TAT→TGT)	ybiB	putative family 3 glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/15);  total (17/15)

CGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTT  >  NC_000913/835801‑836088                                                                                                                                                 |                                                                                                                                                cGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTaa                                                                                                                                           <  1:98543/151‑1 (MQ=255)        ggCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAg                                                                                                                                    <  1:181038/151‑1 (MQ=255)         gCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAgc                                                                                                                                   <  2:410248/151‑1 (MQ=255)              gCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGc                                                                                                                              <  2:554767/151‑1 (MQ=255)                  tttGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGGGGGCGCTACTAATGCATGGGACTGAAGGGGAAGTGGGTGGTAATCCGCAGCGCTGccc                                                                                                                            >  2:377908/1‑149 (MQ=255)                            gTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGGGCTACTTATGCATGGTACTGAAGGTGGAGTGGGGGCTAATCCGCAGCGccgcccgccaat                                                                                                                      >  2:109283/1‑141 (MQ=255)                                   gcTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCt                                                                                                                                <  2:133510/128‑1 (MQ=255)                                     tGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGGGCTACTAATGGCTGGGACTTTAGGGGGAGGGGGGGGCAATCCGCAGCGCCGCCCGCAGATCAATTTCCtttaac                                                                                                        >  1:154650/1‑146 (MQ=255)                                       cGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTg                                                                                                     <  1:234978/151‑1 (MQ=255)                                            ttCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAg                                                                                                  <  2:86549/149‑1 (MQ=255)                                                    tttCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTAATTAAGGGGGAGGGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATATCATTTACCGGGGAGGGGTGCgggg                                                                                        >  2:330680/1‑150 (MQ=255)                                                     ttCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGt                                                                                        <  1:570199/150‑1 (MQ=255)                                                           tCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAAGGGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGAACAATCTCATTGACCGGGGAGGGAAGGGGGGGCTGt                                                                                   >  2:187194/1‑149 (MQ=255)                                                                 aTACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATTCATGGTAATGAAGGGGAAGGGTGTGGTAATTCCCCGCCCTGCCCCCAGAACAATCTCATTTACCGTGAAGGGATGGGGGGGCTGTATGaaaa                                                                            >  2:546030/1‑150 (MQ=255)                                                                      ttGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGGGGAGTGGGTGCTAATCCGCAGCGCTGCCCCCAGATCAAACTCATTTACCGTGAAGGGGTGCGGGGGCTGTATGaaaaaaa                                                                         >  2:393578/1‑146 (MQ=255)                                                                                cgcgAAGTTCTTTAGCGATATCGGCGGGCGGGGGGCACCTATAGCGGGGAAGGAAAGGGAAGAGGGTGGGGAAGCCCCGCGCTGCCCGCAGATCAATTTCATTGACCGGGAAGGGGTGGGGGGGCTGGAATaaaaaaaaggcacaaccg                                                              >  1:235500/1‑136 (MQ=255)                                                                                cgcgAAGTTCTTTAGCGATATCGGCGGGCGGGGGCTAATAATGCATGGGACTGAAGGGGAAGTGTGTGGTAAAACGCAGGGCTGCCCGCAGATCAATTTCATTGACCGGGAAGGGATGGGGGGGGGGTATGAAAAAAAGGACACTGCTgg                                                             >  2:670148/1‑150 (MQ=255)                                                                                   gAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGc                                                         <  2:94672/151‑1 (MQ=255)                                                                                    aaGTTCTTTAGCGATATCGGCGGGCGGGCGCTAATTATACATGGTACCGAAGGCGAAGTGGGGGCTCATCCGCAGCGCTGCCCCCAGAGCAATCTCATTGAACGTGAAGGGGTGCGGGTGGTGTATGAAAAAAAAGAAActtc                                                                >  2:93887/1‑141 (MQ=255)                                                                                                   atCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAg                                         <  1:273872/151‑1 (MQ=255)                                                                                                    tCGGCGGGCGGGCGCTACTAATGCATGGTACTGGAGGTGAAGTGTGTGCTAATCCGCCGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGGGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAg                                         >  1:415583/1‑150 (MQ=255)                                                                                                          ggcgggcgCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGa                                  <  1:380051/151‑1 (MQ=255)                                                                                                              ggcgCTACTAATGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCg                              <  1:640128/151‑1 (MQ=255)                                                                                                                       aaTGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCa                       <  2:333382/149‑1 (MQ=255)                                                                                                                       aaTGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACg                     <  1:378988/151‑1 (MQ=255)                                                                                                                       aaTGCATGGTACTGAAGGTGAAGTGGGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACg                     >  1:325020/1‑151 (MQ=255)                                                                                                                         tGCATGGTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGgc                   >  2:643649/1‑151 (MQ=255)                                                                                                                              ggTACTGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGt              <  2:223802/151‑1 (MQ=255)                                                                                                                                   tGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGa           >  1:9931/1‑149 (MQ=255)                                                                                                                                   tGAAGGTGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGAtt         >  2:13513/1‑151 (MQ=255)                                                                                                                                         tGAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGt   >  2:551290/1‑151 (MQ=255)                                                                                                                                          gAAGTGTGTGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGtt  >  1:657927/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                CGAAACTGGCGACGCCATTTGCCGAAGGTGAGGCGCTGCGTCTTTCCAGCGTTTCGCATCCGGAATACATTGGACGCGTCGCGAAGTTCTTTAGCGATATCGGCGGGCGGGCGCTACTAATGCATGGTACTGAAGGTGAAGTGTATGCTAATCCGCAGCGCTGCCCGCAGATCAATCTCATTGACCGTGAAGGGATGCGGGTGCTGTATGAAAAACAGGACACTGCTGGTAGCGAGTTACTGCCACAAGCAAAAGATCCGGAAACCACGGCGCAGTGGATTGAGCGTT  >  NC_000913/835801‑836088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑