Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	847,016	A→G	V35A (GTA→GCA)	glnP ←	glutamine transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	847,016	0	A	G	100.0%	131.2 / NA	36	V35A (GTA→GCA)	glnP	glutamine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/22);  total (14/22)

CGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATC  >  NC_000913/846870‑847151                                                                                                                                                   |                                                                                                                                        cGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGcc                                                                                                                                       >  2:313623/1‑149 (MQ=255) cGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCa                                                                                                                                      <  2:334045/150‑1 (MQ=255)                  aGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGa                                                                                                                    <  2:594969/151‑1 (MQ=255)                    ggCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAgg                                                                                                                  <  1:372252/151‑1 (MQ=255)                        aaataaatAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCg                                                                                                              <  2:234443/151‑1 (MQ=255)                                aaaCATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCcac                                                                                                      <  1:322931/151‑1 (MQ=255)                                 aaCATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCcac                                                                                                      >  2:339411/1‑150 (MQ=255)                                  aCATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAg                                                                                                    <  1:20931/151‑1 (MQ=255)                                         cTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCAtt                                                                                             >  2:75600/1‑151 (MQ=255)                                            gacgacGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTg                                                                                          <  1:323841/151‑1 (MQ=255)                                                  gaTAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCt                                                                                    <  1:371384/151‑1 (MQ=255)                                                   aTAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCtt                                                                                   >  2:512493/1‑151 (MQ=255)                                                   aTAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCtt                                                                                   >  1:379351/1‑151 (MQ=255)                                                     aGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCa                                                                                 <  1:136905/151‑1 (MQ=255)                                                      ggagtgCCGCGGATCACTTCAATAAAGACCAGCGCGGCGTGGTTGGCTATCCAACCTCCGAAGCAGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCa                                                                                 >  1:603510/4‑150 (MQ=255)                                                      ggTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCaa                                                                                <  2:577281/151‑1 (MQ=255)                                                       gtgtGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAAt                                                                               >  2:266969/1‑151 (MQ=255)                                                            ccGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGaa                                                                          <  2:569705/151‑1 (MQ=255)                                                              gcgGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGc                                                                        >  1:332213/1‑151 (MQ=255)                                                                  aTCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGaa                                                                    <  2:293650/151‑1 (MQ=255)                                                                  aTCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGa                                                                     >  1:209207/1‑150 (MQ=255)                                                                    cACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATg                                                                  <  1:100950/151‑1 (MQ=255)                                                                            tAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGcc                                                           >  1:453918/1‑150 (MQ=255)                                                                                   cAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGc                                                    >  2:217660/1‑150 (MQ=255)                                                                                      cgcgACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTc                                                <  2:552466/151‑1 (MQ=255)                                                                                            gTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCa                                          <  1:530030/151‑1 (MQ=255)                                                                                                  ggCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGc                                    >  2:236939/1‑151 (MQ=255)                                                                                                                  aaGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGtt                    >  1:575044/1‑151 (MQ=255)                                                                                                                   aGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTa                   <  1:333623/151‑1 (MQ=255)                                                                                                                   aGGTGCGTGCAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTa                   <  1:354328/151‑1 (MQ=255)                                                                                                                       gcgtgcAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCgt               <  1:330866/151‑1 (MQ=255)                                                                                                                       gcgtgcAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCgt               <  1:512490/151‑1 (MQ=255)                                                                                                                            cAAAACCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGgtgt          <  2:561905/151‑1 (MQ=255)                                                                                                                               aaCCTGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTaaa       <  2:209208/151‑1 (MQ=255)                                                                                                                                   tGCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAaataat   >  1:236972/1‑151 (MQ=255)                                                                                                                                    gCCAGCAATCCGATTGCCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATc  <  2:453923/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                        CGTTAAACGCCATCGGCAGGGCGAAATAAATAAACATCACCTGGACGACGATAGGTGTGCCGCGGATCACTTCAATAAAGACCAGCGCGACGTGGTTGGCTATCCAACCTCCGAAGGTGCGTGCAAAACCTGCCAGCAATCCGATTACCAGACCGCCTGCCAGACCGAGGACCGAAATCCACAGGGTCATTTTGGCACCTTCAATCAGAAGCGGAATGGCAGGCCAGATGGCACTCCAGTCAAACTGCATATGTTGTTCCTGTTACCGTGGTGTAAATAATC  >  NC_000913/846870‑847151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑