Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,116,797	A→G	intergenic (‑215/‑10)	lpxL ← / → yceA	lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase/putative rhodanese‑related sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,116,797	0	A	G	100.0%	159.2 / NA	45	intergenic (‑215/‑10)	lpxL/yceA	lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase/putative rhodanese‑related sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/23);  total (22/23)

AAATTTGTGGCTGAAGACTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGC  >  NC_000913/1116649‑1116937                                                                                                                                                     |                                                                                                                                             aaaTTTGTGGCTGAAGACTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTGAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGcc                                                                                                                                            <  1:219944/151‑1 (MQ=255)                 aCTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAgtg                                                                                                                            <  1:144088/151‑1 (MQ=255)                 aCTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAgtg                                                                                                                            <  2:292586/151‑1 (MQ=255)                    gggCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTa                                                                                                                         <  2:652113/151‑1 (MQ=255)                         aaaTTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACaa                                                                                                                    <  1:607932/151‑1 (MQ=255)                           aTTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAAcc                                                                                                                  <  2:403154/151‑1 (MQ=255)                                      tGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCaa                                                                                                        <  1:283893/150‑1 (MQ=255)                                      tGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAac                                                                                                       >  2:558951/1‑151 (MQ=255)                                                  ataatTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTaaa                                                                                            >  1:6513/1‑150 (MQ=255)                                                    aatTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGc                                                                                         >  1:383024/1‑151 (MQ=255)                                                       ttttAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCa                                                                                       <  2:166633/150‑1 (MQ=255)                                                        tttAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCaa                                                                                      <  2:571261/150‑1 (MQ=255)                                                         ttAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCaaa                                                                                     >  2:502033/1‑150 (MQ=255)                                                          tAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAAt                                                                                   >  1:576366/1‑151 (MQ=255)                                                              gcgcAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGtt                                                                                <  1:206248/150‑1 (MQ=255)                                                               cgcAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTgg                                                                              >  1:77231/1‑151 (MQ=255)                                                                 cAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCt                                                                            <  1:455256/151‑1 (MQ=255)                                                                    aTGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                         <  2:303587/151‑1 (MQ=255)                                                                    aTGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTgag                                                                         <  2:376845/151‑1 (MQ=255)                                                                        aGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcg                                                                     >  1:66490/1‑151 (MQ=255)                                                                         gCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcga                                                                    <  2:40264/151‑1 (MQ=255)                                                                            tAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaacc                                                                 >  1:288283/1‑151 (MQ=255)                                                                             aaaaTGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaacc                                                                 <  2:564249/150‑1 (MQ=255)                                                                                aTGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaaccgcga                                                             <  2:185379/151‑1 (MQ=255)                                                                                aTGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAgcgaaccgcg                                                              >  1:496369/1‑150 (MQ=255)                                                                                          gTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCAttt                                                   >  2:561920/1‑151 (MQ=255)                                                                                          gTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCAttt                                                   >  1:222780/1‑151 (MQ=255)                                                                                            tAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCg                                                 >  1:144006/1‑151 (MQ=255)                                                                                              aTTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGtt                                               <  2:302801/151‑1 (MQ=255)                                                                                                     aTAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACa                                          >  2:423209/1‑149 (MQ=255)                                                                                                      tAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGt                                       >  2:143366/1‑151 (MQ=255)                                                                                                              atatCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTcc                                 <  2:438294/149‑1 (MQ=255)                                                                                                                atCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACAt                             >  2:451481/1‑151 (MQ=255)                                                                                                                  cATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATc                            <  2:352/150‑1 (MQ=255)                                                                                                                  cATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATc                            >  1:644577/1‑150 (MQ=255)                                                                                                                   aTGCCGCGGATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGc                          >  1:560617/1‑151 (MQ=255)                                                                                                                          ggATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCt                   >  1:367340/1‑151 (MQ=255)                                                                                                                           gATTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCt                   >  1:196842/1‑150 (MQ=255)                                                                                                                            aTTTTTACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTaa                 <  2:460071/151‑1 (MQ=255)                                                                                                                               tttACTTTCCCATCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAgg               <  2:49129/150‑1 (MQ=255)                                                                                                                                   cTTTCCCATCTCGCAGGAGCCGTACACCATGTCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGAcc          >  2:417342/1‑151 (MQ=255)                                                                                                                                          aTCTCGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACg   >  1:86382/1‑151 (MQ=255)                                                                                                                                           tctcGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACg   >  1:104980/1‑150 (MQ=255)                                                                                                                                              cGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACg   <  1:155993/147‑1 (MQ=255)                                                                                                                                              cGCAGGAGCCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGc  <  2:499296/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                             AAATTTGTGGCTGAAGACTGGGCGAAATTGCCGCGCTTGTAAATAACAAATAATTTTTAATGCGCAAATGTAGCGTAAAATGTGTGGATGTTAATTATCGATAATTGCTATATCATGCCGCGGATTTTTACTTTCCCATCTCGCAGGAACCGTACACCATGCCAGTGTTACACAACCGCATTTCCAACGACGCGCTAAAAGCCAAAATGTTGGCTGAGAGCGAACCGCGAACCACCATTTCGTTTTACAAGTATTTCCACATCGCCGATCCTAAGGCGACCCGTGACGC  >  NC_000913/1116649‑1116937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑