Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,124,503	T→C	H275R (CAC→CGC)	mdtH ←	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,124,503	0	T	C	100.0%	119.7 / NA	34	H275R (CAC→CGC)	mdtH	multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/14);  total (20/14)

TAAGGTTTCACGCGCAGGCTCGGCAATGATCGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACT  >  NC_000913/1124364‑1124649                                                                                                                                            |                                                                                                                                                   tAAGGTTTCACGCGAAGGCTCGGCAATGAACGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACg                                                                                                                                          >  1:318759/1‑150 (MQ=255)   aGGTTTCACGCGCAGGCTCGGCAATGATCGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGaaa                                                                                                                                       <  1:288651/151‑1 (MQ=255)     gTTTCACGCGCAGGCTCGGCAATGATCGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAAt                                                                                                                                     <  2:560165/151‑1 (MQ=255)            gcgcAGGCTCGGCAATGATCGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTc                                                                                                                              >  2:347378/1‑151 (MQ=255)                 ggCTCGGCAATGATCGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTcc                                                                                                                         >  2:138432/1‑151 (MQ=255)                               cGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAggg                                                                                                           >  2:223850/1‑151 (MQ=255)                                gACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGt                                                                                                          >  1:202984/1‑151 (MQ=255)                                  cccGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGt                                                                                                          >  2:91811/1‑149 (MQ=255)                                  cccGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGt                                                                                                          >  1:596237/1‑149 (MQ=255)                                      atatAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCa                                                                                                    <  2:460896/151‑1 (MQ=255)                                        ataAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCaa                                                                                                   <  2:579962/150‑1 (MQ=255)                                               aGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACg                                                                                           >  2:450891/1‑151 (MQ=255)                                                  cAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACgac                                                                                         >  1:520277/1‑150 (MQ=255)                                                     aTCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACgacagac                                                                                     <  1:643173/151‑1 (MQ=255)                                                       cAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGacac                                                                                   <  1:223850/151‑1 (MQ=255)                                                             gAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTc                                                                              >  2:582476/1‑150 (MQ=255)                                                             gAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCa                                                                             <  1:229333/151‑1 (MQ=255)                                                                   ttgttgCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGc                                                                        >  1:461247/1‑150 (MQ=255)                                                                    tgttgCGGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCAt                                                                      >  2:642039/1‑151 (MQ=255)                                                                    tgttgCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCAt                                                                      >  1:55162/1‑151 (MQ=255)                                                                        gCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACa                                                                   <  2:496467/150‑1 (MQ=255)                                                                            gCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCAt                                                              <  1:486434/151‑1 (MQ=255)                                                                                  gACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACg                                                        <  2:59885/151‑1 (MQ=255)                                                                                          ccACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAggg                                                >  1:153922/1‑151 (MQ=255)                                                                                             ccGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGgcgc                                             <  1:335399/151‑1 (MQ=255)                                                                                                 catcatGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCgcc                                          >  1:588252/1‑150 (MQ=255)                                                                                                                cATTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGAcc                          >  2:250234/1‑151 (MQ=255)                                                                                                                 aTTATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCa                         <  1:661844/151‑1 (MQ=255)                                                                                                                   tATCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCAt                        >  1:150024/1‑150 (MQ=255)                                                                                                                    aTCAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATa                       >  2:377492/1‑150 (MQ=255)                                                                                                                      cAACAGCCCAGCCATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAAtt                    >  2:85699/1‑151 (MQ=255)                                                                                                                                  cATCAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGcagcatcagc        <  2:55161/151‑1 (MQ=255)                                                                                                                                     cAACCGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGcagcatcagcat      <  1:615404/150‑1 (MQ=255)                                                                                                                                        ccGGCGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACt  >  1:401652/1‑151 (MQ=255)                                                                                                                                            |                                                                                                                                                   TAAGGTTTCACGCGCAGGCTCGGCAATGATCGACCCGATATAAAACAGACAAATCAGGGTGAAAAGTTGTTGCAGGCCGCTGACCATGCCCACCGGCATCATGCTTAATGACATTATCAACAGCCCAGCCATCAACCGGTGTTCCAGACGAAAATGCTTTTCACTCCAGCGGGCGATAGGGTAGAGCAACGTTAACGACAGACACGCTTCAATGGCATACATCCATTTAACGGCAGAGGGCGCGCCAGCCACGTCGTTGACCATAATTGGCAGCATCAGCATCACT  >  NC_000913/1124364‑1124649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑