Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,129,249	T→C	L471L (TTG→CTG)	murJ →	putative lipid II flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,129,249	0	T	C	100.0%	103.8 / NA	29	L471L (TTG→CTG)	murJ	putative lipid II flippase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/14);  total (15/14)

GCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGT  >  NC_000913/1129102‑1129395                                                                                                                                                    |                                                                                                                                                     gCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTgg                                                                                                                                                   <  1:271356/151‑1 (MQ=255)        tgctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCAt                                                                                                                                            >  2:614823/1‑151 (MQ=255)         gctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATg                                                                                                                                           >  2:116057/1‑151 (MQ=255)          ctTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATg                                                                                                                                           >  1:523713/1‑150 (MQ=255)           tttACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGcc                                                                                                                                         >  2:557468/1‑151 (MQ=255)                tGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGc                                                                                                                                    >  2:180382/1‑151 (MQ=255)                 ggCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCg                                                                                                                                   <  2:251227/151‑1 (MQ=255)                    aGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGttt                                                                                                                                >  1:23389/1‑151 (MQ=255)                          gTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCg                                                                                                                          >  1:210759/1‑151 (MQ=255)                          gTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCg                                                                                                                          <  1:81826/151‑1 (MQ=255)                             aGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGttt                                                                                                                       <  2:189350/151‑1 (MQ=255)                               cAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTa                                                                                                                      >  1:604302/1‑150 (MQ=255)                                aGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAAt                                                                                                                    >  1:419498/1‑151 (MQ=255)                                 gAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATg                                                                                                                   >  2:276825/1‑151 (MQ=255)                                   aaaTCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGc                                                                                                                 >  1:406477/1‑151 (MQ=255)                                          tACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGGGAGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCgggcctg                                                                                                          >  1:304945/1‑146 (MQ=255)                                               cGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGc                                                                                                     <  2:220385/151‑1 (MQ=255)                                                       ggCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTg                                                                                            <  2:276696/151‑1 (MQ=255)                                                          tGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑ggggggt                                                                                             >  2:335626/1‑144 (MQ=255)                                                             aTGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑ggggg                                                                                               >  2:594347/1‑141 (MQ=255)                                                                           gCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTg                                                                       <  2:604308/151‑1 (MQ=255)                                                                                         cGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCtt                                                         <  1:645704/151‑1 (MQ=255)                                                                                             aCTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCaa                                                      <  2:650345/150‑1 (MQ=255)                                                                                                             gTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGccc                                     <  2:101322/151‑1 (MQ=255)                                                                                                                  tttAGGTATGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCccgcc                                  <  1:335623/149‑1 (MQ=255)                                                                                                                          tGTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGGGCTGGCGGGGGGTTGCC‑CGGTCTTCCCCGCCCCTGGGGGGACGGGGGTTCAAAATTAAAAAATTTTCCCCCCggggggg                           >  2:306786/1‑144 (MQ=255)                                                                                                                           gTTACATATCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTaa                       <  2:253914/151‑1 (MQ=255)                                                                                                                                  atCATGCCGGAGTGGTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGc                 <  1:228467/150‑1 (MQ=255)                                                                                                                                                gTCACTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt  <  1:585548/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GCTTCGCTGCTTTACTGGCAGTTGCGTAAGCAGAAAATCTTTACCCCGCAACCCGGCTGGATGGCGTTTCTGTTGCGTCTGGTGGTGGCGGTACTGGTGATGTCTGGCGTGCTTTTAGGTATGTTACATATCATGCCGGAGTGGTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGC‑GGGGATTGCCGCGTACTTCGCTG‑CACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGT  >  NC_000913/1129102‑1129395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑