Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	125,921	C→T	S76F (TCC→TTC)	aceF →	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	125,921	0	C	T	100.0%	98.0 / NA	27	S76F (TCC→TTC)	aceF	pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/15);  total (12/15)

GAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATC  >  NC_000913/125779‑126063                                                                                                                                               |                                                                                                                                               gAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGAc                                                                                                                                         <  2:383812/150‑1 (MQ=255)  aaGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGGATCGTTAAAAAGATCAAAATCTCTGTTGGCGATAAAAACCAGACCCGCGCACTGATTATGATTTTTGATTTTGCCGACgg                                                                                                                                       >  2:643694/1‑151 (MQ=37)   aGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTGTCTGTTGGCGATAAAAACCCTACCGGCGCACTGATTATGATTTTCGGTTTCGCCGACg                                                                                                                                        >  1:596088/1‑149 (MQ=37)            gTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGAc                                                                                                                             <  1:643684/151‑1 (MQ=255)                         gTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTc                                                                                                                <  2:116146/151‑1 (MQ=255)                           aGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCa                                                                                                               <  2:529971/150‑1 (MQ=255)                                   aCAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGGATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCCGACCGGCGCACTGATTATGATTTTTGATTTTGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCag                                                                                                          >  1:355599/1‑147 (MQ=255)                                        gCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAgaagagaaga                                                                                                 <  1:114420/151‑1 (MQ=255)                                            ctATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGaagaaa                                                                                               <  1:441861/149‑1 (MQ=255)                                                 gAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCCAAGGCTCTGTTGGGGATAAAACCCACACCGGCGCACTGATTATGTTTTTTGATTTCGCCGACGGCGCAGCAGACGCTCCCCCTCCTCAGGCAGAAGGGAagaaagaa                                                                                            >  1:374438/1‑147 (MQ=255)                                                  aaGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAagcagc                                                                                       <  1:368935/151‑1 (MQ=255)                                                       ccGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCgg                                                                                  <  1:100215/151‑1 (MQ=255)                                                       ccGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCg                                                                                   <  2:402916/150‑1 (MQ=255)                                                             ccGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagc                                                                              <  2:114326/149‑1 (MQ=255)                                                               gCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGgcagcagca                                                                          <  1:231168/151‑1 (MQ=255)                                                                 aGGCGGGCATCGTTAAAGAAATTAAAATCTCTGTTGGCGGTAAAACCCAGACCGGCGCACTTTTTATGATTTTCAATTTCGCCGACGGTGTTTCAGACGCTGCAACCTCTCAGACAGAAGGGAAGAAAGAAGCAGCTCCGGCAGCAGCAcc                                                                        >  1:466282/1‑151 (MQ=255)                                                                               aaaGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTgcggcg                                                           <  2:452097/150‑1 (MQ=255)                                                                               aaaGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGcggcgg                                                          >  2:474555/1‑151 (MQ=255)                                                                                      tCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCaaa                                                      >  2:184816/1‑148 (MQ=255)                                                                                       cAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGa                                                   <  1:457146/150‑1 (MQ=255)                                                                                             ctctGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAAc                                            >  1:369765/1‑151 (MQ=255)                                                                                                 gTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGt                                          <  2:659018/149‑1 (MQ=255)                                                                                                  ttGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTcc                                       >  2:505505/1‑151 (MQ=255)                                                                                                                         gcACTGATTATGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGtt                 >  1:68943/1‑150 (MQ=255)                                                                                                                           aCTGATTATGGTTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGa               >  1:140804/1‑150 (MQ=255)                                                                                                                                  aTGATTTTCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCg       >  1:139523/1‑151 (MQ=255)                                                                                                                                        ttCGATTTCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATc  <  2:81693/150‑1 (MQ=255)                                                                                                                                               |                                                                                                                                               GAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCCTCTATGGAAGTTCCGTCTCCGCAGGCGGGTATCGTTAAAGAGATCAAAGTCTCTGTTGGCGATAAAACCCAGACCGGCGCACTGATTATGATTTTCGATTCCGCCGACGGTGCAGCAGACGCTGCACCTGCTCAGGCAGAAGAGAAGAAAGAAGCAGCTCCGGCAGCAGCACCAGCGGCTGCGGCGGCAAAAGACGTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATC  >  NC_000913/125779‑126063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑