Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,154,782	A→G	E224G (GAA→GGA)	yceG →	septation protein, ampicillin sensitivity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,154,782	0	A	G	100.0%	136.1 / NA	37	E224G (GAA→GGA)	yceG	septation protein, ampicillin sensitivity
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/13);  total (24/13)

TTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACAC  >  NC_000913/1154640‑1154929                                                                                                                                               |                                                                                                                                                    ttACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGAt                                                                                                                                              >  1:324064/1‑150 (MQ=255) ttACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGAt                                                                                                                                              >  1:449792/1‑150 (MQ=255)     tCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGt                                                                                                                                         >  2:582666/1‑151 (MQ=255)                cacaAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAt                                                                                                                              >  1:241218/1‑151 (MQ=255)                   aaGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGGGGAGGGTCTGCCTTATAAAGATAAAACCCAGTGGGTGAGGATGGCATCAATTATCGAAAAAAAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTAt                                                                                                                              <  1:79279/148‑1 (MQ=255)                         aTGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAAcc                                                                                                                     >  2:175755/1‑151 (MQ=255)                           ggTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAAcc                                                                                                                     <  2:36351/149‑1 (MQ=255)                               aaaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAACCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTa                                                                                                                <  2:136416/150‑1 (MQ=255)                                aaGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACg                                                                                                              >  2:639075/1‑151 (MQ=255)                                 aGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGc                                                                                                             >  2:121532/1‑151 (MQ=255)                                              gcCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGcc                                                                                                >  2:230956/1‑151 (MQ=255)                                               ccTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCt                                                                                               >  1:315949/1‑151 (MQ=255)                                               ccTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCt                                                                                               >  1:84466/1‑151 (MQ=255)                                                  gggAAGGGGGTGGGGAGGGTCTACTTTATAAATATAAAACCCAGTGGTTAAGGAGGGAATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTg                                                                                              <  1:129505/149‑1 (MQ=255)                                                     aggggCGTGCGGACGGTCTGCCTTATAAAGATAAAACCCAGTTGGGGACGATGGCACCAATTACCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAga                                                                                          <  2:127414/148‑1 (MQ=255)                                                             gCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                                  >  2:204290/1‑150 (MQ=255)                                                             gCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGAccgacccg                                                                                  >  2:199261/1‑150 (MQ=255)                                                                      cTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGAttt                                                                        >  1:34940/1‑151 (MQ=255)                                                                        gCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATAAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGAtt                                                                         <  1:301390/148‑1 (MQ=255)                                                                          cTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACgg                                                                    >  1:42401/1‑151 (MQ=255)                                                                                   aTAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATggg                                                              >  2:157932/1‑148 (MQ=255)                                                                                     aaaaaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGc                                                         <  2:330184/151‑1 (MQ=255)                                                                                        aaCCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGtt                                                      >  1:317461/1‑151 (MQ=255)                                                                                                        tGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtc                                      >  2:650022/1‑151 (MQ=255)                                                                                                         ggCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACttt                                       <  1:121529/149‑1 (MQ=255)                                                                                                           cATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtctc                                    <  1:639065/150‑1 (MQ=255)                                                                                                           cATCAATTATCAAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtctc                                    <  1:146145/150‑1 (MQ=255)                                                                                                            aTCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTtctc                                    >  2:595728/1‑149 (MQ=255)                                                                                                                     cGAAAAAGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTg                         >  2:134457/1‑151 (MQ=255)                                                                                                                       aaaaaGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTgg                        >  1:6669/1‑150 (MQ=255)                                                                                                                           aGAAACCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACg                   <  2:144970/151‑1 (MQ=255)                                                                                                                             aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc                 >  2:280023/1‑151 (MQ=255)                                                                                                                             aaaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGcc                 <  2:658946/151‑1 (MQ=255)                                                                                                                              aaCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg                >  2:120388/1‑151 (MQ=255)                                                                                                                               aCCGCCGTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCg                >  1:623881/1‑150 (MQ=255)                                                                                                                                    cgTTGCCAGTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCg          <  1:638451/151‑1 (MQ=255)                                                                                                                                            gTGGACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAacac  >  2:29372/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                                    TTACTCAAGCGAGCGCACAAGAAAATGGTGAAAGCGGTCGATAGCGCCTGGGAAGGGCGTGCGGACGGTCTGCCTTATAAAGATAAAAACCAGTTGGTGACGATGGCATCAATTATCGAAAAAGAAACCGCCGTTGCCAGTGAACGCGATAAGGTTGCCTCAGTATTTATCAACCGTTTACGCATTGGTATGCGCCTGCAGACCGACCCGACCGTGATTTACGGGATGGGAGAGCGTTATAATGGCAAACTTTCTCGTGCAGACCTGGAAACGCCGACAGCGTATAACAC  >  NC_000913/1154640‑1154929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑