Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,203,225 C→T intergenic (+1/‑43) croE → / → ymfL e14 prophage; putative DNA‑binding transcriptional regulator/e14 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,203,2250CT98.2% 201.4 / ‑6.3 54intergenic (+1/‑43)croE/ymfLe14 prophage; putative DNA‑binding transcriptional regulator/e14 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (26/27);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAGTTGGCTCAACACCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGCAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGCTGAAGCGGCTG  >  NC_000913/1203077‑1203370
                                                                                                                                                    |                                                                                                                                                 
aaaaGTTGGCTCAACACCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATtagtag                                                                                                                                                 <  1:42671/151‑1 (MQ=255)
 aaaGTTGGCTCAACACCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGa                                                                                                                                                <  2:433525/151‑1 (MQ=255)
        gCTCAACACCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACttt                                                                                                                                         <  1:463135/151‑1 (MQ=255)
            aaCACCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAAt                                                                                                                                     <  2:32700/151‑1 (MQ=255)
             acacCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAAtt                                                                                                                                    >  1:91011/1‑151 (MQ=255)
              cacCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAAttt                                                                                                                                   >  2:159132/1‑151 (MQ=255)
               acCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAAtttt                                                                                                                                  <  2:144249/151‑1 (MQ=255)
                ccTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAAttttt                                                                                                                                 <  1:252121/151‑1 (MQ=255)
                ccTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAAttttt                                                                                                                                 <  1:291146/151‑1 (MQ=255)
                  tGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTa                                                                                                                                >  1:381250/1‑150 (MQ=255)
                  tGGCTACTCACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTa                                                                                                                                >  2:584448/1‑150 (MQ=255)
                   ggCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTaa                                                                                                                               <  2:346457/150‑1 (MQ=255)
                   ggCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGCAGAACACTTTCAATTTTTAAc                                                                                                                              <  1:182647/151‑1 (MQ=255)
                     cTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAAcc                                                                                                                             >  2:532550/1‑150 (MQ=255)
                       aCTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACATTCAATTTTTAACca                                                                                                                            >  2:447039/1‑149 (MQ=255)
                           aCGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAAc                                                                                                                      >  1:604900/1‑151 (MQ=255)
                            cGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACg                                                                                                                     >  2:634999/1‑151 (MQ=255)
                            cGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACg                                                                                                                     <  1:391407/151‑1 (MQ=255)
                              gCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGAt                                                                                                                   <  1:293963/151‑1 (MQ=255)
                               cTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGAt                                                                                                                   >  1:378156/1‑150 (MQ=255)
                                  gTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAgg                                                                                                               >  2:169505/1‑151 (MQ=255)
                                   ttttCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGc                                                                                                              >  1:29449/1‑151 (MQ=255)
                                           ggCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGt                                                                                                       <  2:128369/150‑1 (MQ=255)
                                           ggCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTg                                                                                                      <  2:265351/151‑1 (MQ=255)
                                               gtaAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGt                                                                                                   >  1:638395/2‑150 (MQ=255)
                                                taAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTa                                                                                                  >  2:293627/1‑150 (MQ=255)
                                                   aaaaGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCa                                                                                              <  1:483397/151‑1 (MQ=255)
                                                      aGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCa                                                                                           <  1:52753/151‑1 (MQ=255)
                                                        ccAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACt                                                                                         <  1:603929/151‑1 (MQ=255)
                                                                gtgCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAAt                                                                                  <  1:506422/150‑1 (MQ=255)
                                                                     ggCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGaaaa                                                                            <  2:51654/151‑1 (MQ=255)
                                                                     ggCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGaaa                                                                             >  2:168208/1‑150 (MQ=255)
                                                                         aaaaaaCTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAAc                                                                          <  2:228920/149‑1 (MQ=255)
                                                                           aaaaCTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGcc                                                                      >  2:558427/1‑151 (MQ=255)
                                                                                  gAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTg                                                                <  1:634989/150‑1 (MQ=255)
                                                                                      aaTACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACg                                                           >  2:619653/1‑151 (MQ=255)
                                                                                       aTACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGt                                                          >  1:670829/1‑151 (MQ=255)
                                                                                         acacATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGa                                                        <  2:394572/151‑1 (MQ=255)
                                                                                              tCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCt                                                   >  1:354936/1‑151 (MQ=255)
                                                                                                aGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGt                                                 >  1:348918/1‑151 (MQ=255)
                                                                                                 ggTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTc                                                <  1:312843/151‑1 (MQ=255)
                                                                                                      aaTTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGaaa                                           <  1:159129/151‑1 (MQ=255)
                                                                                                       aTTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGaaaa                                          <  1:472713/151‑1 (MQ=255)
                                                                                                            gAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTAt                                     >  1:10785/1‑151 (MQ=255)
                                                                                                                   gACTTAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATcgc                                  <  2:657404/147‑1 (MQ=255)
                                                                                                                       tAAGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGc                          >  2:346006/1‑151 (MQ=255)
                                                                                                                         aGACCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCg                        <  1:317151/151‑1 (MQ=255)
                                                                                                                           aCCGGATATCTATCCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAg                      <  1:324624/151‑1 (MQ=255)
                                                                                                                                   tctatcCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACg              >  2:516473/1‑151 (MQ=255)
                                                                                                                                    ctatcCGAAAGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGc             >  2:383666/1‑151 (MQ=255)
                                                                                                                                           aaaGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGCTGAAGCg      >  1:596394/1‑151 (MQ=255)
                                                                                                                                            aaGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGCTGAAGCgg     <  2:294688/151‑1 (MQ=255)
                                                                                                                                            aaGATTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGCTGAAGCgg     >  1:306578/1‑151 (MQ=255)
                                                                                                                                               aTTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGCTGAAGCGGCt   <  1:237360/150‑1 (MQ=255)
                                                                                                                                               aTTAGTAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGCTGAAGCGGCTg  >  2:76662/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
AAAAGTTGGCTCAACACCTGGCTACTTACGGCTGGTTTTCAATGGCTATAAAAAAGCCAGTTTTGTGCTGGCTAAAAAACTTGAGCAATACACATCAGGTGCAATTACGAAATCTGACTTAAGACCGGATATCTATCCGAAAGATTAGCAGAACACTTTCAATTTTTAACCACAGAACGATGAGGCTAATCGTGGGTAAGCATCACTGGAAAATAGAAAAACAGCCTGAGTGGTACGTGAAAGCTGTCAGAAAAACTATCGCGGCGTTGCCGAGTGGTTACGCTGAAGCGGCTG  >  NC_000913/1203077‑1203370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: